Ask about this productRelated genes to: Lpcat2 antibody
- Gene:
- LPCAT2 NIH gene
- Name:
- lysophosphatidylcholine acyltransferase 2
- Previous symbol:
- AYTL1
- Synonyms:
- FLJ20481, AGPAT11, LysoPAFAT
- Chromosome:
- 16q12.2
- Locus Type:
- gene with protein product
- Date approved:
- 2005-11-03
- Date modifiied:
- 2015-08-26
Related products to: Lpcat2 antibody
Related articles to: Lpcat2 antibody
- Metastatic melanoma is an aggressive, heterogeneous cancer with early spread and poor prognosis. Transcriptomic analysis identifies potential therapeutic targets. In silico analysis of the GEO dataset GSE7553 compared primary vs metastatic melanoma using differential expression, enrichment (GO/KEGG/Reactome), PPI network construction, and hub-gene prioritization. Candidates were validated through survival analysis, mutation-associated analyses, and virtual screening using molecular docking with FDA-approved compounds. Transcriptomic results show divergence between primary and metastatic melanoma samples, with principal component analysis supporting clear group separation. In a total of 54,675 probe-level entries, 4868 were classified as upregulated and 10,269 as downregulated, indicating a predominance of downregulated transcriptional events in metastatic melanoma. Prioritized upregulated genes included CUL5, ZC3H14, SON, BRCC3, and H3-3B, whereas notable downregulated genes included ZNF709, CD84, STARD8, EPOR, and HAVCR2. The high-confidence PPI network comprised 625 nodes and 2661 edges, with a significant enrichment score. Enrichment analysis implicated immune/adhesion and translation pathways (e.g., Rap1, focal adhesion, T-cell activation). Survival: CUL5 (HR = 0.26) and ZC3H14 (HR = 0.60) are protective, while SON (HR = 2.4) is adverse. Mutation-linked transcriptomic analysis identified 10 significantly altered genes, including downregulated SNHG18 and upregulated LPCAT2. Virtual screening results show repurposable compounds, with Floxacrine showing strong predicted affinity for CUL5 and Dihydroergocristine showing favorable interaction with LPCAT2/ZC3H14-related targets. In silico docking results further supported CUL5-Floxacrine and LPCAT2-Dihydroergocristine as notable candidate interactions. Results show key transcriptomic drivers and targets (CUL5, ZC3H14, SON, BRCC3, LPCAT2) in metastatic melanoma. Results highlight a useful hypothesis-generating framework for biomarker prioritization and drug repurposing in melanoma. However, independent cohort validation and experimental confirmation are required before clinical translation. - Source: PubMed
Publication date: 2026/05/02
Majeed Khulood AyadMajeed Raghad AyadIbrahim Taisir KhalilKhan Najeeb Ullah - Granulomas are organized inflammatory lesions formed in response to persistent stimuli such as infections. Murine infection with Leishmania donovani results in granulomas in the liver, seeded by infected Kupffer cells, and serves as a well-defined model of infection-induced granuloma formation. The resolution of granulomatous inflammation requires dynamic shifts in immune-cell activation states, imposing metabolic demands. As mediators of cell signaling, lipid metabolism plays a key role in regulating inflammation and infection. How lipid changes are spatially linked to altered immune cell transcription remains unresolved. We performed a multimodal imaging analysis combining MALDI mass spectrometry, spatial and single cell transcriptomics, proteomics of flow-sorted macrophages and histopathology of L. donovani induced hepatic granulomas. Using this spatially-integrated approach, we identified LPCAT2-mediated membrane re-modelling of myeloid cells as a novel feature of these granulomas. Our study provides new insights into local immunometabolic changes associated with granuloma formation and macrophage activation. - Source: PubMed
Publication date: 2026/03/20
Dey ShoumitCao Jian-HuaBalluff BenjaminMazza GaiaAshwin HelenGilbert LesleyJames SallyDowle Adam ACalder GrantDey Nidhi SharmaO'Toole PeterHeeren Ron M AKaye Paul M - Meat quality traits are typically regulated by multiple genes, each contributing a small effect. In this study, to pinpoint candidate genes involved in meat quality traits, we performed transcriptome profiles of porcine (LD) muscle and applied machine learning (ML) models to analyze RNA-seq data. We also carried out Gene Set Enrichment Analysis (ssGSEA), Weighted gene co-expression network analysis (WGCNA) and functional validation of putative target genes to better support the biological relevance of our findings. - Source: PubMed
Publication date: 2026/03/14
Ma ShuyaHu XiezongYang JianweiShi KunpengZhang XiaodongDing YueyunWu XudongZhu MengtingYin ZongjunZheng Xianrui - Lysophosphatidylcholine acyltransferase (LPCAT) is a key enzyme of the Lands cycle that remodels phosphatidylcholine (PC). Proper balance between lysophosphatidylcholine (LPC) and PC is essential for phospholipid turnover and membrane homeostasis. Here, we show that the Arabidopsis lpcat double mutant exhibits an altered LPC/PC ratio in root tissues, reflecting a compromised Lands cycle. Using a chemical phenocopying strategy, we found that root growth of the lpcat mutant was more sensitive than that of wild type to lysoPAF, a structural analog of LPC. This defect was largely rescued when lysoPAF treatment was combined with ONO-RS-082, a phospholipase A2 (PLA2) inhibitor. Subcellular localization experiments with GFP fusions demonstrated that both LPCAT1 and LPCAT2 are targeted to the endoplasmic reticulum (ER) membrane. Furthermore, RNA-seq analysis of root tissues revealed transcriptional changes indicative of ER stress and altered endomembrane trafficking. Together, our results demonstrate that ER-localized LPCAT plays a pivotal role in maintaining root growth integrity when plants encounter conditions that require adjustments in lipid homeostasis. The likely role of ER localized LPCAT in modulating ER stress and root growth will be discussed. - Source: PubMed
Publication date: 2026/01/09
Wang LipingKazachkov MichaelQin LiNiu YuxingWei YangdouLi QiangZou Jitao - Elevated glycolysis in lung tissue is a hallmark of sepsis-induced acute lung injury (SI-ALI), yet the role of glycolytic reprogramming and lactate-derived protein modifications in damaging epithelial cells remains poorly understood. In this study, we reveal that PDK4-driven glycolytic reprogramming promotes excessive lactate production in lung tissue during SI-ALI. Mechanistically, AARS1 in epithelial cells selectively enhances lactylation modification at the K375 site of LPCAT2, which suppresses STAT1 acetylation and facilitates STAT1 phosphorylation, nuclear translocation, and transcriptional repression of SLC7A11. This cascade ultimately triggers epithelial cells ferroptosis. Pharmacological inhibition of PDK4 attenuates lactate accumulation and LPCAT2 lactylation, thereby restoring STAT1 acetylation and SLC7A11 expression. Furthermore, AARS1 knockdown or mutation of the LPCAT2-K375 lactylation site rescues STAT1-mediated SLC7A11 suppression and mitigates ferroptosis in vitro and septic mice. Our findings revealed that elevated expression of PDK4 is a critical factor contributing to the increased lactate production in lung tissue during sepsis, and established a novel LPCAT2-K375/STAT1/SLC7A11 axis driving epithelial cells ferroptosis in SI-ALI, highlighting the crosstalk between metabolic reprogramming, post-translational modifications (PTM), and ferroptosis. Targeting the PDK4 or LPCAT2 lactylation may offer therapeutic potential for SI-ALI. In sepsis-induced acute lung injury (SI-ALI), PDK4 hyperactivation drives excessive lactate production in epithelial cells, triggering AARS1/HDAC9-mediated LPCAT2 lactylation. This modification suppresses STAT1 acetylation while enhancing phosphorylation, driving its nuclear translocation and subsequent SLC7A11 transcriptional downregulation. The resultant glutathione synthesis deficiency promotes ferroptosis, exacerbating SI-ALI progression. - Source: PubMed
Publication date: 2025/10/07
Deng YifanQiu YuetanLi XiangGong TingGuo JinyanLiang HaoxuanYuan ZiyiHei ZiqingZhang XuediLiu Youtan