Ask about this productRelated genes to: ZNF580 antibody
- Gene:
- ZNF580 NIH gene
- Name:
- zinc finger protein 580
- Previous symbol:
- -
- Synonyms:
- -
- Chromosome:
- 19q13.42
- Locus Type:
- gene with protein product
- Date approved:
- 2004-02-15
- Date modifiied:
- 2015-09-10
Related products to: ZNF580 antibody
Related articles to: ZNF580 antibody
- Ischemeia-reperfusion (I/R) injury is a severe complication after restoring blood perfusion in acute myocardial infarction treatment, in which vascular endothelial cell dysfunction is considered as the key event to exacerbate myocardial injury. We have previously verified the protective function of ZNF580 in endothelial cells, however, the impact of ZNF580 on I/R injury and its underlying mechanisms have not been explored in depth. The purpose of the present study is to investigate the regulatory role of ZNF580 on myocardial I/R injury and confirm that ZNF580 is a potential therapeutic candidate for I/R injury treatment. The potential mechanism of ZNF580 in I/R injury was determined via bioinformatics. A model of I/R injury in human umbilical vein endothelial cells (HUVECs) was subsequently established to confirm whether ZNF580 protects against I/R injury and whether this protective effect is exerted through the regulation of autophagic flow. Our study identified 459 differentially expressed genes (DEGs) in I/R injury. ZNF580 increased cell viability and gradually restored cell morphology, the cytoplasm was full, the intracellular structure was clear, and the cell space was significantly reduced in HUVECs exposed to I/R injury. Both Western blotting and reverse transcription-polymerase chain reaction (RT-qPCR) were used to detect the levels of different apoptosis-related proteins, it is shown that the ZNF580 significantly increased lysosome-associated membrane protein 2 (LAMP2) and light chain 3 (LC3) expressions, and markedly decreased protein 62 (P62) expression. Moreover, ZNF580 decreased lactate dehydrogenase (LDH) levels in the supernatant and the rate of apoptosis. ZNF580 promoted autophagosome and lysosome fusion and increased autophagic flux, thereby protecting HUVECs from I/R injury. Its protective effect is possibly related to the activation of the adenosine 5'-monophosphate (AMP)-activated protein kinase (AMPK) signaling pathway. - Source: PubMed
Publication date: 2025/02/19
Zhang ZhongbaiQin XuetingLiu JiyuanYan ZhiyunXie HongweiZhang Mei - In this study, a targeted graphene quantum dot-cationic polymer composite gene vector with endothelial cell-targeting CAG peptide was successfully designed and prepared. This vector could efficiently bind and deliver the therapeutic gene pZNF580 to endothelial cells (HUVECs). At a concentration of less than 40 μg mL, the results of the CCK-8 assay showed that the relative cell viability of each composite gene vector was greater than 80%, and the results of the flow cytometry assay showed that C-GQDs-PEI-PEG-CAG/pZNF580 (88.96%) and N-GQDs-PEI-PLGA-PEG-CAG/pZNF580 (87.70%) treated groups showed significantly higher cell viability than the positive control group Lip2000/pZNF580 (56.76%). The results of cell transfection and western blot experiments confirmed that the composite gene vector was able to deliver pZNF580 efficiently and enable the high expression of the ZNF580 protein in HUVECs. The results of the EdU assay, wound healing and Transwell experiments indicated that the composite gene vector/pZNF580 nanoparticles (NPs) could significantly promote the proliferation and migration. The results of the EdU method showed that the proliferative ability of C-GQDs-PEI-PLGA/pZNF580 (84.96 ± 1.99%) and N-GQDs-PEI-PLGA/pZNF580 (85.01 ± 1.31%) treatment groups for HUVECs was significantly higher than that of the positive control group Lip2000/pZNF580 (77.89 ± 2.18%). The results of the scratch assay showed that the cell migration rate of C-GQDs-PEI-PLGA-PEG-CAG/pZNF580 (93.08 ± 1.97%) and N-GQDs-PEI-PLGA-PEG-CAG/pZNF580 (91.99 ± 1.52%) groups was significantly higher than that of the positive control group Lip2000/pZNF580 (85.03 ± 2.21%). In addition, the results of the angiogenesis assay showed that the C-GQDs-PEI-PLGA-PEG-CAG/pZNF580 and N-GQDs-PEI-PLGA-PEG-CAG/pZNF580 groups had significantly higher angiogenesis-promoting ability than the positive control group, Lip2000/pZNF580.The present study provides a highly efficient and low-toxic method to promote endothelial cell migration in the field of regenerative medicine and a low-toxicity strategy to promote endothelial layer formation, which provides new possibilities for future vascular regeneration therapy. - Source: PubMed
Publication date: 2024/07/31
Duo XinghongXu QirongLi ChenMeng XiangyanFeng Yakai - Medullary thyroid carcinoma (MTC) is a rare but aggressive endocrine malignancy that originates from the parafollicular C cells of the thyroid gland. Enhancer RNAs (eRNAs) are non-coding RNAs transcribed from enhancer regions, which are critical regulators of tumorigenesis. However, the roles and regulatory mechanisms of eRNAs in MTC remain poorly understood. This study aims to identify key eRNAs regulating the malignant phenotype of MTC and to uncover transcription factors involved in the regulation of key eRNAs. - Source: PubMed
Liu DaxiangWang WenjingWu YanzhaoQiu YongleZhang Lan - Endothelial cell proliferation plays an important role in angiogenesis and treatment of related diseases. The aim of this study was to evaluate the effect of polyethylenimine (PEI)-modified graphene quantum dots (GQDs) gene vectors on endothelial cell proliferation. The GQDs-cationic polymer gene vectors were synthesized by amidation reaction, and used to deliver pZNF580 gene to Human umbilical vein endothelial cells (HUVECs) for promoting their proliferation. The chemical modification of GQDs can adjust gene vectors' surface properties and charge distribution, thereby enhancing their interaction with gene molecules, which could effectively compress the pZNF580 gene. The CCK-8 assay showed that the cell viability was higher than 80% at higher vector concentration (40 μg/mL), demonstrating that the GQDs-cationic polymer gene vectors and their gene complex nanoparticles (NPs) having low cytotoxicity. The results of the live/dead cell double staining assay were consistent with those of the CCK-8 assay, in which the cell viability of the A-GQDs/pZNF580 (94.38 ± 6.39%), C-GQDs-PEI- polylactic acid-co-polyacetic acid (PLGA)/pZNF580 (98.65 ± 6.60%) and N-GQDs-PEI-PLGA/pZNF580 (90.08 ± 1.60%) groups was significantly higher than that of the Lipofectamine 2000/pZNF580 (71.98 ± 3.53%) positive treatment group. The results of transfection and western blot experiments showed that the vector significantly enhanced the delivery of plasmid to HUVECs and increased the expression of pZNF580 in HUVECs. In addition, the gene NPs better promote endothelial cell migration and proliferation. The cell migration rate and proliferation ability of C-GQDs-PEI-PLGA/pZNF580 and N-GQDs-PEI-PLGA/pZNF580 treatment groups were higher than those of Lipofectamine 2000/pDNA treatment group. Modified GQDs possess the potential to serve as efficient gene carriers. They tightly bind gene molecules through charge and other non-covalent interactions, significantly improving the efficiency of gene delivery and ensuring the smooth release of genes within the cell. This innovative strategy provides a powerful means to promote endothelial cell proliferation. - Source: PubMed
Publication date: 2024/02/24
Xu QirongLi ChenMeng XiangyanDuo XinghongFeng Yakai - Hepatocellular carcinoma (HCC) is the most common type of liver malignancy with high incidence and poor prognosis. Transmembrane protein 147 (TMEM147) has been implicated in the development of colon cancer. However, the role of TMEM147 in HCC remains unclear. In this study, data of 371 HCC tissues, 50 adjacent nontumor tissues, and 110 normal liver tissues were retrieved from the TCGA and GTEx databases. TMEM147 expression was found to be increased in HCC tissues. High expression of TMEM147 was related to poor prognosis, and TMEM147 was confirmed to be an independent prognostic factor for HCC patients. A receiver operating characteristics (ROC) analysis was performed and showed that the diagnostic efficacy of TMEM147 was significantly higher than that of AFP (0.908 versus 0.746, p < 0.001). Furthermore, TMEM147 promoted tumor immune infiltration, and macrophages were the immune cells that predominantly expressed TMEM147 in HCC. Further analysis revealed that TMEM147 mainly impacted the ribosome pathway, and CTCF, MLLT1, TGIF2, ZNF146, and ZNF580 were predicted to be the upstream transcription factors for TMEM147 in HCC. These results suggest that TMEM147 serves as a promising biomarker for diagnosis and prognosis and may potentially become a therapeutic target for HCC. - Source: PubMed
Publication date: 2023/06/16
Fan Wen-JieZhou Meng-XiWang Di-DiJiang Xin-XinDing Hao