Ask about this productRelated genes to: MBD2 antibody
- Gene:
- MBD2 NIH gene
- Name:
- methyl-CpG binding domain protein 2
- Previous symbol:
- -
- Synonyms:
- -
- Chromosome:
- 18q21.2
- Locus Type:
- gene with protein product
- Date approved:
- 1999-01-11
- Date modifiied:
- 2016-10-05
Related products to: MBD2 antibody
Related articles to: MBD2 antibody
- The Wnt signaling pathway antagonist SFRP1 is frequently silenced by promoter DNA hypermethylation in colorectal cancer (CRC). MBD2, a DNA methylation reader, is known to contribute to SFRP1 epigenetic silencing. Previous work showed that MBD2 critically suppresses SFRP1 expression without altering promoter methylation, though the underlying mechanism remained unclear. Elucidating how DNA methylation silences tumor suppressor genes, such as , could reveal novel therapeutic targets with significant clinical potential. - Source: PubMed
Publication date: 2026/05/05
Huang XiujiLuo TingtingKe LinglingGuan ShaotingWu HuixianLi BoLiu YutingQi Jian - Fertility is a multifactorial trait and a key determinant of productivity and sustainability in beef cattle production. Identifying molecular mechanisms and biomarkers associated with fertility could improve the prediction of reproductive potential in beef heifers. Herein, by combining transcriptomic and proteomic data from peripheral white blood cells (PWBCs) collected before the time of artificial insemination (AI), we investigated molecular differences between fertile and subfertile beef heifers (n = 6 per group) classified based on their reproductive outcomes. RNA-Sequencing and untargeted proteomics identified 230 differentially expressed genes (DEGs; ≤ 0.05 and |log2FC| ≥ 0.5) and 70 differentially abundant proteins (DAPs; ≤ 0.05) between groups. Over-representation analyses revealed that these molecules were associated with cell cycle regulation, metabolism, and immune-related pathways, including chemokine and JAK-STAT signaling ( ≤ 0.01). Data integration revealed limited overlap between DEGs and DAPs ( and ). Among these, expression was previously reported to be progesterone-responsive, supporting its potential role in early pregnancy establishment. Network analyses revealed distinct regulatory patterns between groups (|r ≥ 0.95| and ≤ 0.05). At the transcript level, subfertile heifers exhibited increased connectivity, indicating potential compensatory transcriptional rewiring. We identified 92 regulatory impact factor (RIF) genes with potential modulatory roles, including . Epigenetic transcription factors, including and were also rewired, suggesting an interplay between hormone signaling and chromatin regulation that modulates transcript expression and consequently fertility outcomes. Our results show that PWBCs reflect systemic molecular changes associated with fertility status and represent a promising, non-invasive source for biomarker discovery. This integrative multi-omics approach provided novel insights into the regulatory networks underlying fertility in beef heifers, highlighting the value of integrating multi-omics to identify key pathways and molecular targets to improve reproductive efficiency in beef production systems. - Source: PubMed
Publication date: 2026/04/20
Brown T CodyBanerjee PriyankaDyce Paul WFalkenberg ShollieRodning Soren PDiniz Wellison J S - Synaptic activity results in long-lasting alterations of neuronal properties, which require gene expression regulation. PYK2 is a calcium-activated non-receptor protein tyrosine kinase highly expressed in hippocampal neurons and involved in synaptic functions. PYK2 also shuttles between the nucleus and the cytoplasm. We show that glutamate stimulation induces PYK2 accumulation in the nucleus of hippocampal neurons in culture through activation of NMDA receptors, L-type voltage-gated Ca channels, and calcineurin. NMDA receptor stimulation also increases nuclear location and interaction with PYK2 of methyl-CpG binding domain protein 2 (MBD2), a modulator of histone modifications and nucleosome remodeling. In PYK2-KO neurons, MBD2 nuclear translocation is diminished, acetylation of histone H4-Lys5 is decreased, and methylation of histone H3-Lys4 is increased. The transcriptome is modified in PYK2-KO hippocampus with a decreased expression of genes coding for excitatory synaptic proteins. In cultured neurons, the absence of PYK2 enhances the glutamate-induced downregulation of synaptic protein transcripts related to epilepsy pathophysiology. In wild-type mice, pilocarpine-induced status epilepticus increases PYK2 and MBD2 nuclear localization in hippocampal neurons, especially in CA3. In PYK2-KO mice, aberrant synaptic sprouting and cell death triggered by status epilepticus are reduced in CA3 compared to wild-type littermates. In PYK2-KO neurons in culture, glutamate-induced cell death is attenuated, and this effect is abolished by re-expression of wild-type PYK2 but not of mutated PYK2 unable to translocate to the nucleus. In summary, our study indicates that regulated PYK2 nuclear translocation in hippocampal neurons may facilitate transcription by removing MBD2 from the active chromatin and may contribute to seizure-induced neurotoxicity. - Source: PubMed
Publication date: 2026/04/22
Giralt AlbertMendes TiagoMontalban EnricaCifuentes-Diaz CarmenGalán-Ganga MarcosChouchana Margotde Pins BenoîtLongueville SophieCarrel DamienGirault Jean-Antoine - Autism spectrum disorder (ASD) remains underexplored in Southeast Asia, with limited characterization of its molecular underpinnings and clinical heterogeneity. This study investigated transcriptomic variation and its relationship to clinical diversity in Thai children with ASD using integrated molecular and phenotypic analyses. - Source: PubMed
Publication date: 2026/04/14
Yuwattana WasanaPoolcharoen ChayanitSaeliw Thanitvan Erp Marlieke LisanneKanlayaprasit SongphonVanwong NatchayaHu Valerie WTrairatvorakul PonChonchaiya WeerasakLê Cao Kim-AnhSarachana Tewarit - The increasing prevalence of mycotoxin toxicity poses significant health risks, contributing to various diseases. Among these, fumonisin B1 (FB) alters sphingolipid biosynthesis, induces oxidative stress, apoptosis, mitochondrial dysfunction, and inflammation. This study investigated the impact of acute FB exposure on inflammation and epigenetics changes in hearts of C57BL/6 mice. Molecular docking was performed to identify potential interactions between FB and key inflammatory proteins (TNF-α, iNOS, NF-κB p65, and NF-κB p50). Excised C57BL/6 mice heart tissue was analysed for gene expression (qPCR), protein expression (Western blotting), nitric oxide levels (NOS assay), cytokine levels (ELISA), and global DNA methylation (ELISA). Molecular docking suggested FB interacted with key residues in TNF-α, iNOS, and NF-κB, potentially influencing their activity. Gene expression analysis (TNF-α, NF-κB, IL-6, NLRP3 Inflammasome, IL-18, caspase 1, IL-1β, GSDMD, caspase 3, CT-1, IL-10, MBD2, DNMT1, DNMT3A, and DNMT3B) revealed that FB significantly dysregulated inflammatory cytokines and DNA methylation-related genes. Protein expression analysis showed significant upregulation of pro-inflammatory cytokines (TNF-α, NF-κB, IL-6, IL-1β, IL-18, IL-10, and TGF-β1). Global DNA methylation levels were significantly increased, with notable upregulation of DNMT1. In conclusion, acute exposure of C57BL/6 mice to FB significantly impacted inflammatory and DNA methylation pathways, leading to cardiac distress complications. - Source: PubMed
Publication date: 2026/03/31
Gounder SelwynMhlongo NdumisoGhazi TerishaChuturgoon Anil