Ask about this productRelated genes to: DNMT1 antibody
- Gene:
- DNMT1 NIH gene
- Name:
- DNA methyltransferase 1
- Previous symbol:
- DNMT
- Synonyms:
- MCMT, CXXC9
- Chromosome:
- 19p13.2
- Locus Type:
- gene with protein product
- Date approved:
- 1991-06-04
- Date modifiied:
- 2019-04-23
Related products to: DNMT1 antibody
Related articles to: DNMT1 antibody
- DNA methylation, is catalyzed by DNA methyltransferases (DNMTs), and its aberrant patterns are implicated in thyroid cancer pathogenesis. The study aimed to investigate the association of DNMTs with thyroid cancer and evaluated the effects of sustained demethylating therapy in a cell-based study. DNMTs expressions in thyroid cancer were analyzed using GEO and TCGA datasets. Additionally, 16 paired and three unpaired papillary thyroid carcinoma (PTC) samples from Taipei Medical University (TMU), along with commercial tissue arrays, were analyzed. Furthermore, the effects of the covalent DNMT inhibitor, 5-azacytidine (5-Aza), and the DNMT1-selective inhibitor, GSK-3484862, on cell viability were evaluated in PTC and follicular thyroid carcinoma (FTC) cell lines. DNMT1 and DNMT3A were upregulated in PTC, with DNMT1 expression correlated with the BRAF mutation and lymph node invasion in TCGA data, findings further confirmed in the TMU cohort and tissue arrays. Short-term (24 h) 5-Aza treatment (1 and 5 µM) induced substantial cell death regardless of the DNA methylation status, whereas short-term GSK-3484862 (5 µM) treatment showed minimal cytotoxicity. In contrast, sustained low-dose GSK-3484862 treatment (approximately 1-3 weeks at 2 µM) effectively reduced global DNA methylation and decreased cell viability of TPC-1 and FTC-236 cells through apoptosis, rather than by inhibiting proliferation. In conclusion, DNMT1 overexpression in PTC suggests its involvement in thyroid carcinogenesis. Sustained inhibition of DNMT1 effectively reduced global DNA methylation and promoted apoptosis, highlighting the potential of prolonged DNMT1-targeted therapy. Further in vitro and in vivo studies are warranted to validate these results and elucidate the underlying mechanisms. - Source: PubMed
Cheng Chao-WenFang Wen-FangWang Yuan-HungYang Yea-MeyLin Jiunn-Diann - Maternal nutrition during critical windows of development plays a pivotal role in shaping long-term disease susceptibility, including cancer risk. This study investigated whether maternal exposure to lipotropes (methyl donor nutrients) during pregnancy and lactation modulates gene expression in 7,12-dimethylbenzanthracene (DMBA)-induced mammary tumors in adult female offspring. Timed-pregnant Sprague-Dawley rats were fed with either a control or lipotrope-supplemented diet, with or without vitamin B6. Female offspring were exposed to DMBA at puberty, and mammary tumors were evaluated histologically and molecularly. DMBA-induced tumors displayed ductal carcinoma in situ-like morphology and significant upregulation of fetal mammary developmental genes ( and ), the tumorigenesis-associated gene , and key epigenetic regulators (, , and ). Estrogen receptor 1 () mRNA expression also showed a significant increase. Maternal lipotropes supplementation significantly attenuated the expression of these genes in offspring tumors. Collectively, these findings demonstrate that maternal methyl donor nutrition modulates tumor-associated gene expression patterns, potentially by limiting the reactivation of developmental and epigenetic pathways in adulthood. This study highlights maternal nutrition as a modifiable early-life factor with important implications for long-term health programming. - Source: PubMed
Publication date: 2026/04/19
Mabasa LawrenceKotze AnriJohnson RabiaRamharack PritikaOmoruyi Sylvester IGabuza Kwazikwakhe BSharma JyotiWillmer Tarryn - DNA methylation at the C-5 position of cytosine is an important epigenetic mechanism underpinning various cellular functions, such as heterochromatin assembly, gene expression, and cell fate determination. In mammals, DNA methylation mainly occurs in the context of CpG dinucleotide contexts. Establishment and maintenance of mammalian DNA methylation is orchestrated by two groups of functionally distinct enzymes: DNA methyltransferases DNMT3A and DNMT3B and maintenance DNA methyltransferase DNMT1. For proper genomic methylation, both and maintenance DNMTs are subjected to multilayered regulation by the chromatin environment, such as histone modifications and the methylation stiate of the CpG dinucleotide. Furthermore, DNA methylation is critically regulated by the accessory proteins of DNMTs, such as DNMT3L and DNMT3B3 for methylation and E3 ubiquitin ligase UHRF1 for maintenance DNA methylation. Increasing structural, biochemical and cellular evidence has unveiled the intricate interplay between the conformational dynamics of DNMTs and their target specification in governing the dynamic DNA demethylation across the genome. This review focuses on recent advances in structural and functional understanding of DNMTs, emphasizing how the interplay between their intramolecular and intermolecular interactions modulates the conformational dynamics and function of the individual DNA methylation machinery, thereby shaping the dynamic DNA methylation landscape across the mammalian genome. - Source: PubMed
Publication date: 2026/04/24
Song Jikui - The effects of maternal broccoli powder (BP) intake on inflammation and AMPK activation in weaning offspring programmed by maternal undernutrition remain poorly understood. This study aimed to investigate whether maternal BP intake during lactation ameliorates inflammation and affects AMPK phosphorylation in the hypothalamus and liver of weaning offspring subjected to maternal undernutrition. Pregnant rats received either a normal protein (NP, 20% casein) or a low protein (LP, 8% casein) diet. During lactation, dams were provided with either a normal protein diet without or with 0.74% BP (NP/NP or NP/NPBP) or a low protein diet without or with 0.74% BP (LP/LP or LP/LPBP). Blood, liver (left lateral lobular region), and hypothalamic samples (region estimated to include the arcuate nucleus and ventromedial hypothalamus) were collected on postnatal day 21. In the liver, macrophage count, NFκB p65 protein expression, and TNF-α mRNA expression were lower in LP/LPBP than in LP/LP. In the hypothalamus, Iba1 mRNA expression, NFκB p65 protein expression, and TNF- mRNA expression were reduced in LP/LPBP compared to LP/LP. AMPK phosphorylation was upregulated in both the liver and hypothalamus of LP/LPBP offspring relative to LP/LP. In the liver, mTOR and Akt phosphorylation were downregulated in LP/LPBP compared to LP/LP. Additionally, Dnmt1 levels were lower in LP/LPBP than in LP/LP in the liver, whereas in the hypothalamus, Dnmt1 and Dnmt3a mRNA expression levels were higher in NP/NPBP than in NP/NP. In conclusion, maternal BP intake during lactation decreased inflammation and increased AMPK phosphorylation in the liver and hypothalamus of weaning rats programmed by maternal undernutrition. - Source: PubMed
Publication date: 2026/04/27
Karmacharya AnishmaKasai ShihoMukai YuukaSato Shin - Wnt4 signaling promotes somatic cell development in the female embryo, but its role in germline differentiation during meiosis remains poorly characterized. To explore Wnt4 functions in female embryonic gonads, we isolated germ cells from Wnt4 knock-out mice to investigate histone modifications and DNA methylation distribution patterns. The lack of the Wnt4 signaling pathway deregulates germ cell cycle markers, such as cyclins, alters the cell cycle by impairing meiosis progression, maintains the germ cells in the G1-GO and S phases, and supporting DNMT3A and DNMT1 enzyme expression at meiosis entry. Conversely, in the nucleus of the Wnt4 knock-out female germ cells, an increase of H3K27me3 pattern persists at the entry of meiosis, leading to altered methylation at the Sycp3 promoters combined with an acetylation of Stra8 promoter at E14.5. This changed pattern might be explained by the overexpression of Creb-binding protein (CBP) in the mutant female germ cells, leading to deregulation of histone marks on meiosis genes. Our findings reveal that the Wnt4 signal is necessary for inducing meiosis by inhibiting germ cell proliferation via the regulation of histone modification. Wnt4 signaling plays a crucial role in regulating the delicate balance between DNA methylation and acetylation in female germ cells. This fascinating interaction highlights the complexities of cellular processes that contribute to reproductive health and development. - Source: PubMed
Publication date: 2026/04/22
Naillat FlorenceTomizawa Shin-IchiIvanova ElenaKelsey GavinVainio Seppo J