Ask about this productRelated genes to: eNOS antibody
- Gene:
- NOS3 NIH gene
- Name:
- nitric oxide synthase 3
- Previous symbol:
- -
- Synonyms:
- ECNOS, eNOS
- Chromosome:
- 7q36.1
- Locus Type:
- gene with protein product
- Date approved:
- 1993-08-23
- Date modifiied:
- 2016-10-05
Related products to: eNOS antibody
Related articles to: eNOS antibody
- Patients with ST-segment elevation myocardial infarction (STEMI) frequently experience no-reflow (NRF) during percutaneous coronary intervention (PCI). Nevertheless, the pathogenesis of NRF involves multiple factors and has not been fully elucidated to date. Given that endothelial dysfunction, inflammation, and oxidative stress are jointly implicated in both MP-related processes and NRF occurrence, this study was performed to explore the potential role of circulating microparticles (MPs) in the development of NRF. - Source: PubMed
Li Wen-BoZhang WeiChang Feng-JunLi ZheYang Yu-JuanCheng GongJiang Xin - Danshen-Chuanxiong is a traditional herb pair used for cardiovascular and cerebrovascular disorders, but its chemical basis and potential effects in Alzheimer's disease (AD)-related endothelial injury remain incompletely defined. Here, an integrated analytical workflow combining reversed-phase liquid chromatography (RPLC) and hydrophilic interaction liquid chromatography (HILIC) with quadrupole time-of-flight mass spectrometry (Q-TOF-MS), cellular assays, metabolomics, and network pharmacology was applied in an amyloid-β (Aβ) oligomer-induced human brain microvascular endothelial cell (hBMEC) injury model. The complementary RPLC/HILIC and positive/negative electrospray-ionization strategy expanded coverage across a broad polarity range. UHPLC-Q/TOF-MS detected 90 compounds, mainly phenolic acids, phthalides, tanshinones, diterpenoids, and other aromatic or terpenoid constituents. The reductions in cell viability and zonula occludens-1 expression following Aβ exposure confirmed the successful establishment of the cellular injury model, whereas pretreatment with Danshen, Chuanxiong, or their combination increased cell viability and alleviated Aβ-induced cellular injury. Untargeted metabolomics annotated 38 differential metabolites, of which 30 showed a recovery-associated shift toward the control state after Danshen-Chuanxiong treatment; the associated pathways included amino-acid, purine, glutathione, and glycerophospholipid metabolism. Integrative analysis prioritized seven candidate targets of Danshen-Chuanxiong, namely ACHE, VEGFA, NOS2, NOS3, AKR1B1, MDM2, and XDH. These findings provide a separation-MS-based chemical characterization and mechanistic framework for further validation of Danshen-Chuanxiong in AD-related endothelial injury models. - Source: PubMed
Wang HuiLi LingHe XiaoliShen ShuqiChao LiangYou PiaoxueJi KaihongLiu YueHong Zhanying - Vascular cognitive impairment (VCI) is a vast disease with chronic cerebral hypoperfusion (CCH) being the major driver. In our previous study, we have verified that Da ChuanXiong (DCX) formula could alleviate VCI, and obviously upregulate the decreased cerebral blood flow, indicating its potential benefits on vascular remodeling. This study aims to explore the pro-angiogenic and vasodilatory effects of DCX formula after CCH injuries, and clarify its pharmacodynamic substances and potential mechanisms. The rat model of CCH was established by bilateral common carotid artery occlusion. CD31 immunofluorescent staining and quail chorioallantoic membrane (qCAM) experiments were conducted to assess the angiogenic effect of DCX. Isolated vascular ring experiments and NO assay in qCAM were performed to evaluate the vasodilatory effect of DCX. Then we analyzed the chemical components absorbed in the blood of DCX under CCH conditions by UPLC-Q-TOF/MS, and further combined network pharmacology, molecular docking to predict the potential mechanisms and effective components. Western blot was performed in CCH rats to verify the possible mechanisms and in vitro pharmacodynamic verification experiment was conducted for innovative active ingredients in DCX. Our results revealed that DCX obviously increased the number of CD31 cells in the hippocampus and brain cortex of CCH rats, and significantly increased the vascular areas in qCAM. DCX significantly dilated the pre-constricted vascular ring and increased NO contents in qCAM. Besides, Senkyunolide A, Butylphthalide, Ferulic acid, Senkyunolide I, Levistilide A, Ligustilide, Senkyunolide H and Gastrodin were identified as the 8 major blood-absorbed compounds of DCX under CCH condition. Next, network pharmacology and molecular docking revealed NOS3 and PI3K/Akt signaling as the critical targets and pathway of DCX improving vascular remodeling against CCH injuries, which was further validated by western blot. Moreover, we firstly discovered that Senkyunolide A and Senkyunolide H could obviously promote cell migration and enhance the tube formation ability of HUVECs in vitro. Taken together, DCX formula effectively enhanced the angiogenesis and vasodilation via activating PI3K/Akt/eNOS signaling, which might be the important pathways for DCX promoting vascular remodeling against CCH injuries. Notably, Senkyunolide A and Senkyunolide H were firstly identified as innovative components responsible for the pro-angiogenic effects of DCX. - Source: PubMed
Publication date: 2026/09/03
Gao JialeLou TianyuHuang BeibeiGuo XiuyingWu HaoLiu JieLi YuetingFeng MenghanLiu LirongXiao Hongbin - γ-Aminobutyric acid (GABA)-producing lactic acid bacteria are promising natural alternatives for hypertension management. Here, we isolated a novel high GABA-yielding strain, Lactobacillus buchneri XBMU-C1 (1.45 g/L), from traditional homemade sauerkraut. The strain exhibited excellent tolerance to acid (83.6% survival at pH 3.0), bile salts (85.1% survival at 1 g/L after 4 h), and simulated gastrointestinal fluid (12.2% survival). In an Nω-Nitro-L-arginine methyl ester (L-NAME) induced hypertensive rat model, oral administration of XBMU-C1 fermentation broth for 6 weeks dose-dependently reduced mean arterial pressure (MAP), with the high dose (150 mg·kg·d) lowering MAP by 22.15 mmHg. Mechanistically, the fermentation broth significantly decreased serum levels of angiotensin-converting enzyme (ACE) and angiotensin II (Ang II), while increasing serum nitric oxide (NO) content. Western blot analysis revealed upregulated protein expression of phosphatidylinositol 3-kinase (PI3K), protein kinase B (Akt), and endothelial nitric oxide synthase (eNOS) in vascular tissues. Histopathological examination showed that the fermentation broth alleviated hypertension-induced myocardial injury, vascular smooth muscle hyperplasia, and intestinal villus damage. Collectively, Lactobacillus buchneri XBMU-C1 fermentation broth exerts antihypertensive effects through dual modulation of renin-angiotensin-aldosterone system (RAAS) suppression and PI3K/Akt/eNOS-mediated NO enhancement, offering a safe and effective natural intervention for hypertension. - Source: PubMed
Publication date: 2026/07/18
Gao DandanWang ChenchenChen YingWang JiwenMei JunyaoCao YujieYang XuankangKang ShangyunMa JinyongLi Ping - Chronic obstructive pulmonary disease (COPD)-associated sarcopenia is a critical comorbidity associated with high morbidity and mortality, driven by chronic systemic inflammation, oxidative stress, and proteometabolic dysregulation. The (HQ-RS) herbal pair is a traditional remedy for consumptive diseases; however, its molecular mechanisms against this comorbidity remain undefined. - Source: PubMed
Publication date: 2026/08/27
Wu XiaowenLi JiaxiangHe JiananYu ZhiyueGao MingGeng Limei