Ask about this productRelated genes to: FOXA2 antibody
- Gene:
- FOXA2 NIH gene
- Name:
- forkhead box A2
- Previous symbol:
- HNF3B
- Synonyms:
- -
- Chromosome:
- 20p11.21
- Locus Type:
- gene with protein product
- Date approved:
- 1998-02-11
- Date modifiied:
- 2016-10-05
Related products to: FOXA2 antibody
Related articles to: FOXA2 antibody
- Efficient delivery of large gene-editing plasmids, such as the mCherry-CRISPR dCas9 system, into nucleus pulposus (NP) cells is a key step in generating sufficient cells for tissue-engineered intervertebral disc (IVD) constructs and other regenerative therapies for degenerative disc disease (DDD). However, the transfection and transduction of these environmentally sensitive cells remain challenging. This study aimed to identify the best protocol for delivery with minimal cytotoxicity and greatest efficiency. Transfection conditions in HEK293T cells were evaluated using Lipofectamine 3000, Lipofectamine Classic, and ViaFect at different reagent-to-DNA ratios and DNA amounts. Transfection efficiency was quantified by flow cytometry based on mCherry expression. Lentivirus was produced and concentrated by comparing PEG8000, a commercial Lenti-X concentrator, and ultracentrifugation. For NP cell transduction, polybrene and protamine sulfate were tested at multiple concentrations to maximize efficiency and viability. The optimized protocol was validated by delivering a CRISPR/dCas9 Synergistic Activation Mediator (SAM) system to activate endogenous FOXA2, and by seeding the resulting cells onto membranes to assess NP-like tissue formation. Lipofectamine 3000 at a 2:1 reagent-to-DNA ratio with 0.5 µg DNA per well yielded the highest transfection efficiency in HEK293T cells while minimizing cytotoxicity. Coprecipitation methods for lentiviral concentration, particularly the in-house PEG8000 concentrator, were better than ultracentrifugation. Protamine sulfate at 30 µg/mL yielded efficient NP cell transduction with higher viability than polybrene, as assessed by survival after antibiotic selection. Application of this protocol upregulated endogenous FOXA2 mRNA and protein expression, demonstrating functional efficacy. SAM-FOXA2 cells produced thicker tissue on membrane inserts than SAM controls, confirming compatibility of the protocol with downstream tissue engineering applications. Therefore, an optimized protocol balancing high transfection/transduction efficiency with minimized cytotoxicity was developed, supporting tissue-engineered IVD constructs and other CRISPR-based regenerative therapies for DDD. - Source: PubMed
Publication date: 2026/08/19
Davison Kotler EvanAshraf SajjadSanterre J PaulKandel Rita A - The progressive lung function decline in severe asthma is poorly explained by traditional inflammation‑centered paradigms. Calcium (Ca²⁺) dysregulation and transforming growth factor‑β (TGF‑β) signaling have been implicated, but the molecular link between them and their upstream regulators remain elusive. - Source: PubMed
Publication date: 2026/08/14
Zhu ZhiqinXiao QingaoCai RunjinQi EnyuGong XiaoxiaoTang JialeLi XiaozhaoFeng Juntao - Adult stem cells are thought to drive the regenerative potential of the endometrium and contribute to the pathogenesis of endometriosis; however, their identity and defining features remain to be characterized. Here, we used in vivo and in vitro approaches to demonstrate that cells with high aldehyde dehydrogenase 1 activity (ALDH cells) were long-lived progenitors in the endometrium with a higher organoid formation capacity, long-term passaging potential, and stemness gene signatures. Using lineage tracing with an reporter mouse, epithelial cells expanded during postnatal development, stromal cells expanded during estrous cycling, and both populations of cells were present during postpartum repair. In response to ovariectomy or exogenous estradiol, we found that ALDH1A1 cells localized to glandular crypts of the endometrium or throughout the luminal epithelium, respectively, indicating that their spatial localization is hormone-sensitive. Functionally, we found that selective ablation of ALDH1A1 cells in mice decreased endometrial gland number and FOXA2 expression. These findings were recapitulated in the human endometrium, where endometrial epithelial organoids with high ALDH activity (ALDH cells) showed a higher organoid formation capacity than ALDH cells and displayed unique transcriptomes with fewer luminal-like ciliated cells. Overall, our studies indicate that ALDH1A1 cells are hormone-sensitive adult stem cells in the endometrium with regenerative potential that are critical for endometrial development and function. - Source: PubMed
Publication date: 2026/08/06
Tang SuniUnser Anna CatherineJiang PeixinParks Sydney EHerrera Genesis JGeng TingAlpuing Radilla LindaThigpen Brooke AGuan XiaomingMonsivais Diana - This study explores a stem-cell-based approach for diabetes treatment by enhancing the viability and functionality of insulin-producing cells (IPCs) derived from stem cells of the apical papilla (SCAP). Although SCAP can differentiate into IPCs, limited cell survival remains a challenge. To address this, the proliferation enhancer WS6 was incorporated into Eudragit RS100 nanoparticles (NPs) using microfluidics. The WS6-loaded NPs were characterized for size, charge, PDI, morphology, stability, and drug loading. An MTT assay was performed as a preliminary screening method to evaluate the cytocompatibility of blank-NPs and to optimize treatment concentration. SCAP cells were treated with free WS6 or WS6-loaded NPs, and cellular uptake of NPs was evaluated using flow cytometry and fluorescence imaging. Additionally, the viability of treated cells was determined by propidium iodide (PI) and trypan blue. Prior to differentiation, definitive endoderm formation was assessed through SOX17 and FOXA2 expressions. After differentiation into IPCs, maturation markers such as insulin, C-peptide, PDX-1, NKX2.2, and NKX6.1 were examined, and apoptosis assays measured cell viability. Functional insulin secretion was tested using an in vitro glucose-stimulated insulin secretion (GSIS) assay. Results showed that WS6-loaded NPs significantly improved SCAP viability, increased healthy cell percentages, and enhanced IPC maturation. Treated IPCs demonstrated functional insulin secretion and improved glucose regulation. Overall, WS6-loaded NPs represent a promising approach to enhance IPC proliferation and generation for diabetes therapy. - Source: PubMed
Publication date: 2026/08/04
Abuarqoub DuaaMohammad MarwaAlbarghouthi RandAbuoun MohammadAlnatour MohammadAlhawarat FuadJaradat Abdolelah - Within the present study, the aim was to investigate the detailed mechanism of forkhead box protein A2 (FOXA2) in anoikis resistance in lung adenocarcinoma (LUAD). The levels of FOXA2 and ATP-binding cassette subfamily A member 8 (ABCA8) were assessed using public databases. The effects of overexpression (oe)-FOXA2, oe-nicotinamide adenine dinucleotide phosphate oxidase 4 (NOX4) and sh-ABCA8 on NOX4 levels, aggregation, cell viability, apoptosis and apoptosis-related proteins were analyzed using reverse transcription quantitative PCR, microscopy, MTT assays, flow cytometry and western blotting. Chromatin immunoprecipitation and dual-luciferase reporter assays were employed to determine the interaction between FOXA2 and ABCA8. In LUAD, FOXA2 and ABCA8 mRNA levels were downregulated, with lower expression observed in in A549 cells compared with BEAS-2B cells; this pattern was pronounced under suspension culture, where A549 cells showed a further decrease in FOXA2/ABCA8 expression alongside a marked increase in NOX4 expression, compared with adherent culture. Furthermore, oe-ABCA8 inhibited NOX4 expression and reversed the effects of oe-NOX4 on enhancing cell aggregation, promoting cell viability, decreasing apoptosis, suppressing cleaved caspase-3 expression and increasing NOX4 level in suspended A549 cells. Furthermore, FOXA2 bound to the ABCA8 promoter region and oe-FOXA2 reversed the effects of ABCA8 silencing on suspended A549 cells, including enhanced cell aggregation, increased cell viability, reduced apoptosis, suppressed cleaved caspase-3 expression and elevated NOX4 expression. Collectively, FOXA2 enhanced ABCA8 transcription, leading to inhibition of NOX4 expression and subsequently alleviating anoikis resistance in A549 cells. - Source: PubMed
Publication date: 2026/07/22
Hu NinaLi LingHao JianqingLi LifangPan MengmengHe Jia