Ask about this productRelated genes to: TUBB3 antibody
- Gene:
- TUBB3 NIH gene
- Name:
- tubulin beta 3 class III
- Previous symbol:
- FEOM3
- Synonyms:
- beta-4, CFEOM3, CFEOM3A
- Chromosome:
- 16q24.3
- Locus Type:
- gene with protein product
- Date approved:
- 2004-11-22
- Date modifiied:
- 2015-12-17
Related products to: TUBB3 antibody
Related articles to: TUBB3 antibody
- Merkel cells (MCs) are specialized mechanosensory receptor cells essential for touch sensation, exhibiting both epithelial and neuroendocrine characteristics. However, the regulatory processes that shape MC fate remain poorly defined. Using single-cell RNA sequencing, we construct a conserved, triphasic atlas of MC ontogeny comprising an epithelial-like stage, a transitional intermediate phase, and a terminal neuroendocrine state. Importantly, we identify as a priming factor essential for initiating MC differentiation, while acts as a driver of epithelial-neuroendocrine conversion and subsequent functional integration. Moreover, the gene regulatory network analysis further reveals that TUBB3 primed cells could differentiate into the MC lineage under the regulation of and . Genetic lineage tracing and cross-species analyses validate these mechanisms. Together, our work delineates the transcriptional network orchestrating MC fate determination and mechanosensory differentiation, providing not only mechanistic insights but also highlighting actionable targets relevant to MC carcinoma and sensory restoration. - Source: PubMed
Publication date: 2026/09/22
Zhang YajunYuan HuipuJiang YuyanWang TengRui ChenDing YuanyuanWang TuanWang ChaochenXiao Ying - Repair of critical peripheral nerve injuries requires bioactive conduits that provide structural guidance while supporting cellular regeneration. Here, we engineered a biomimetic hydrogel conduit comprising methacrylated pectin (PecMA) functionalized with human hair-derived keratin to integrate fascicle-mimetic architecture with biochemical cues. Keratin was extracted using a urea-based reduction strategy and conjugated to PecMA via thiol-ene chemistry, yielding a dual-crosslinkable bioink. A custom multifilament extrusion platform enabled fabrication of aligned fascicle-like conduits. In vitro, cell-laden constructs demonstrated uniform cellular distribution, high viability, and enhanced neurite extension following keratin incorporation. PC12 neurite length increased from 6.28 ± 0.49 μm on day 1 to 64.77 ± 4.85 μm on day 14, while qRT-PCR revealed increased expression of neuronal (PRPH, TUBB3, NEFH) and Schwann cell/myelination-associated (PMP22, MBP, MPZ) genes compared with PecMA. In vivo, acellular Keratin-PecMA conduits were evaluated in a rat sciatic nerve defect model and demonstrated favourable biocompatibility, aligned axonal regeneration, and reduced fibrotic tissue infiltration. The Keratin-PecMA group showed progressive improvement in SFI, with foot-slip percentage decreasing from 32.5 ± 3.2% at week 2 to 15.0 ± 2.2% at week 8. At 8 weeks, SFI scores (-60.95 ± 2.68 vs. -57.73 ± 3.99), nerve fibre diameter (2.12 ± 0.12 vs. 2.11 ± 0.25 μm), and muscle-to-collagen ratio (3.21 ± 0.06 vs. 2.95 ± 0.25) were comparable between the Keratin-PecMA and autograft groups. Overall, these findings support Keratin-PecMA multifilament conduits as a promising biomimetic strategy for peripheral nerve regeneration. - Source: PubMed
Publication date: 2026/09/16
Ramesh Preethy AmruthavarshiniThangadurai MadhumithraSethuraman SwaminathanSubramanian Anuradha - The stony coral, Acropora tenuis, possesses progenitor/undifferentiated cells similar to other anthozoans; however, their developmental potential and fate remain obscure. The present study initially showed that nerve progenitors and undifferentiated-like cells were both tagged with histone H3 trimethylation at Lys 4 and Lys 27, suggesting that Acropora progenitor/undifferentiated cells might be multipotent. A rabbit anti-reverse transcriptase (anti-AtRTase) antibody recognized undifferentiated-like cells in the middle region of the ectodermal layer in embryos 60-85 h post-fertilization. At the larval stages, AtRTase signals were concentrated around the oral pole where the larval ectoderm invaginated to form the pharyngeal stomodeum. Undifferentiated-like cells, but not the apical-most glandular epithelium in the ectoderm, appeared to enter the pharynx. Consistent with this hypothesis, AtRTase signals were continuous from undifferentiated-like cells to the stomodeum. Nerve progenitors (neuroblasts) expressed nerve-specific tubulin beta-III (TUBB3). They differentiated into nerve cells and expressed a neuroblast differentiation-associated protein around the aboral pole of larvae. On the other hand, non-aboral neuroblasts expressed the autophagy-related protein 10 in addition to TUBB3 and developed into vacuolated hyaline cells (VHCs) that penetrated through the ectoderm apicobasally. The present study suggests strongly that ectodermal undifferentiated cells in A. tenuis have the potential to form the stomodeum. In contrast, nerve progenitors possess the dual potential to differentiate into nerve cells and VHCs, which appears to depend on aboral and non-aboral positions. - Source: PubMed
Kawamura KazSekida SatokoSatoh Noriyuki - The aim of this research was to use Mebendazole as an anticancer candidate to reduce the dose of Flutamide and reduce its side effects. - Source: PubMed
Fattahi Mohammad RezaTaheri DianaInanloo Seyed HassanMirzaei AkramAzodian Ghajar HeliaReis Leonardo OliveiraAghamir Seyed Mohammad Kazem - 2,4,6-Tribromophenol (TBP) is the most environmentally prevalent bromophenol pollutant. Although TBP tends to accumulate in the brain, its neurotoxic effects and underlying mechanisms remain poorly understood. In the present study, the neurodevelopmental toxicity of TBP was investigated using an in vitro neural differentiation model of mouse embryonic stem cells (mESCs). Results showed that during differentiation, TBP exposure suppressed the expression of the ectoderm marker Tfap2α on day 6. Moreover, after 12 days of exposure, TBP significantly reduced the protein expression levels of PAX6 (neural precursor marker) and TUBB3 (neuronal marker) and inhibited acetylcholinesterase (AChE) activity. TBP exhibited limited effects on canonical developmental signaling pathways, such as BMP, WNT, and Notch-Hes, suggesting that its inhibitory effect on neural differentiation may not depend on these pathways. Transcriptomic analysis revealed TBP-induced alterations in both lipid metabolism-related pathways and genes involved in the mitochondrial respiratory chain. Further biochemical validation experiments demonstrated that TBP upregulated the expression of the lipid uptake gene Cd36, leading to increased total cholesterol (TC) and triglyceride (TG) levels. Concurrently, mitochondrial oxidative stress was elevated, whereas mitochondrial membrane potential, complex I activity, and ATP content were markedly decreased. Therefore, TBP likely impaired neural differentiation by disrupting lipid homeostasis and mitochondrial function. This study enhances the understanding of the neurotoxic mechanisms of bromophenol pollutants and provides crucial scientific evidence for their health risk assessment. - Source: PubMed
Publication date: 2026/08/22
Sun YumiaoPei YaoLiu HuinanGao YurouYang XiaoxiLiu Qian SXu LiFaiola FrancescoZhou QunfangJiang Guibin