Ask about this productRelated genes to: PAK2 antibody
- Gene:
- PAK2 NIH gene
- Name:
- p21 (RAC1) activated kinase 2
- Previous symbol:
- -
- Synonyms:
- PAK65, PAKgamma
- Chromosome:
- 3q29
- Locus Type:
- gene with protein product
- Date approved:
- 1998-03-25
- Date modifiied:
- 2016-10-24
Related products to: PAK2 antibody
Related articles to: PAK2 antibody
- Acute myeloid leukemia (AML) is an aggressive hematological malignancy frequently exhibiting deregulated expression/activity/localization of the Wnt signaling mediator β-catenin. To derive more effective β-catenin targeting strategies, we previously interrogated its interaction network in myeloid cells and identified several putative novel interacting partners, including Target of EGR1 (TOE1); a deadenylase with unknown function in hematological tissue. This study aimed to define TOE1 function in hematopoietic cells and uncover its molecular targets. TOE1 interacted with β-catenin in both primary and immortalized AML cells, and impacted Wnt signaling output through the modulation of lymphoid enhancer-binding factor-1 (LEF-1). AML samples exhibited deregulated TOE1 expression versus normal hematopoietic stem/progenitor cells (HSPCs), and TOE1 depletion suppressed the proliferation of myeloid leukemia cell lines, and primary human HSPCs, partly through a p21-activated-kinase 2 (PAK2) mediated mechanism. In summary, these data reveal TOE1 as a novel regulator of hematopoietic cell proliferation via the modulation of important growth-regulating pathways. - Source: PubMed
Publication date: 2026/04/23
Park HyunSevim OkanWagstaff MeganGoff AaronPalmer David AKim BomeeHeesom KateBlair AllisonNewbury Sarah FMorgan Ethan LTowler Benjamin PChevassut Timothy JMorgan Rhys G - Endothelial cells promote thrombosis and haemostasis through the secretion of von Willebrand Factor (VWF) from their secretory granules - the Weibel-Palade bodies (WPBs). In response to specific stimuli, dynamic actin nucleation and remodelling of the cytoskeleton facilitates the expulsion of ultra-large VWF multimers to elevate plasma VWF and to form a platform for platelet capture and thrombus formation. P21 activated kinase 2 (PAK2) is a crucial regulator of the actin cytoskeleton and is essential for VWF secretion in response to secretagogues which utilise actomyosin mediated exocytosis. Here we characterise the role of β-PAK-interacting exchange factor (β-PIX) in WPB exocytosis. Inhibition of β-PIX function prevents dynamic cytoskeletal remodelling resulting in reduced VWF secretion. Depletion of β-PIX using siRNA reduced the number of WPB fusion events, prolonged the time taken for GFP-VWF to be secreted post-fusion and delayed kinetics of exocytic actomyosin ring. Use of full length and truncated β-PIX demonstrated that the PAK interacting and GEF domain mediate cytoskeletal remodelling whereas only the full-length construct could rescue VWF secretion. β-PIX regulates both septin ring formation and cofilin mediated actin remodelling during actomyosin ring function. These data identify β-PIX as a regulator of endothelial exocytosis through supporting actomyosin mediated expulsion of VWF. [Media: see text] [Media: see text]. - Source: PubMed
Publication date: 2026/04/23
El-Mansi SammyDeegan Karishma MNightingale Thomas D - African swine fever (ASF), caused by the African swine fever virus (ASFV), is currently a major threat to the global swine industry and food security because no safe and effective vaccines or antiviral drugs are available. Developing a defense strategy that incorporates antiviral agents and other approaches is urgently needed. Here, we found that a fucoidan, SPW-05-S2, from a brown alga, inhibits ASFV infection by over 95% in porcine alveolar macrophages (PAMs) via suppressing viral entry and factory formation. Using an affinity precipitation workflow, we identified 4 viral and 665 cellular proteins as potential binding partners of SPW-05-S2 in ASFV-infected PAMs, of which 3 viral proteins and 2 cellular proteins (PAK2 and Talin-1) were experimentally validated. Mechanistically, our results suggest that SPW-05-S2 inhibits ASFV replication by both suppressing endocytosis via targeting the PAK2/LIMK/Cofilin/F-actin remodeling pathway and blocking viral factory formation through perturbing Talin-1-mediated cytoskeletal remodeling networks. Collectively, our findings show that SPW-05-S2 blocks ASFV infection by modulating host cytoskeletal pathways at two critical stages of the viral life cycle. Given that fucoidans are already widely used in food and pharmaceuticals, SPW-05-S2 represents a promising, low-risk candidate for antiviral intervention against ASFV. - Source: PubMed
Publication date: 2026/04/02
Wan SiqiSong BaoyiLi SaijuanCao CaiqinLu ManPeng GuiqingDing KanHan KunYu Mei - 'Cutaneous T-cell lymphoma (CTCL), particularly tumor stage mycosis fungoides (MF), presents significant therapeutic challenges due to limited treatment efficacy. This study addresses the unmet need for novel targeted therapies targeting the constitutively hyperactive STAT3/5 pathway. - Source: PubMed
Publication date: 2026/03/17
Dey SaptaswaSorger HelenaSchlederer MichaelaPerchthaler IsabellaMetzelder MartinKenner LukasMoriggl RichardWolf Peter - Although kinase activators hold significant therapeutic promise, their development remains challenging and rarely achieved. Here, we report the discovery of direct small-molecule activators of p21-activated kinase-1 (PAK1), a key regulator of cardiac homeostasis, using a rational peptide-guided strategy. Targeting PAK1 autoinhibitory regulation, we identified a previously unrecognized autoinhibition-release site between the autoregulatory region and the kinase domain. Subsequent high-throughput screening and medicinal chemistry optimization yielded selective allosteric activators that enhance PAK1 activity with micromolar potency and isoform selectivity. Structural and mechanistic analyses indicate that these activators disrupt autoinhibitory regulation and promote local and global conformational transitions to the active state. Enhanced PAK1 signaling was confirmed in cardiac cells, and in vivo studies demonstrated therapeutic efficacy in both inherited and acquired cardiac hypertrophy. Collectively, these findings establish rational modulation of kinase autoinhibitory regulation as a potential strategy for the broader discovery of kinase activators, a largely unexplored area of therapeutic development. - Source: PubMed
Publication date: 2026/03/31
He YuBae James S HNowak ElżbietaOuteiral CarlosNissley Daniel ATumber AnthonyBerridge GeorginaSalah EidarusWang YiHe WenqiZhang HongyuanChen TangtingTusk SamuelMathea SebastianWang Ying-JieGrassam-Rowe AlexanderKukura PhilippSchofield Christopher JO'Brien Darragh PPierangelini AndreaChurchill Grant CLanyon-Hogg ThomasKe YunboXu ChaoYe TaoWatkins HughYing LimingKoschinski AndreasSolaro R JohnTan XiaoqiuBolla Jani RWang XinKnapp StefanDeane Charlotte MZaccolo ManuelaNowotny MarcinLei Ming