Ask about this productRelated genes to: DAZAP1 antibody
- Gene:
- DAZAP1 NIH gene
- Name:
- DAZ associated protein 1
- Previous symbol:
- -
- Synonyms:
- MGC19907
- Chromosome:
- 19p13.3
- Locus Type:
- gene with protein product
- Date approved:
- 1999-12-07
- Date modifiied:
- 2014-11-19
Related products to: DAZAP1 antibody
Related articles to: DAZAP1 antibody
- DAZ‑associated protein 1 (DAZAP1), an RNA‑binding protein and modulator of alternative splicing, participates in tumorigenesis. However, the potential oncogenic function and mechanism of DAZAP1 in gastric cancer (GC) are unknown. Gene expression analysis, including mRNA and protein level assessment by reverse transcription‑quantitative PCR and western blotting, respectively, immunofluorescence, immunohistochemistry, hybridization assays, tissue microarray, RNA immunoprecipitation and sequencing and mRNA stability assay were performed, as well as colony formation, EdU, wound healing, migration and invasion assays of GC cells. DAZAP1 displayed a significant upregulation in GC cells and served as an oncogene, as demonstrated by its overexpression promoting colony formation, EdU incorporation, wound healing, migration and invasion, and its knockdown suppressing these malignant phenotypes. Additionally, DAZAP1 upregulation was positively correlated with tumor progression and poor survival in individuals with GC. Functionally, DAZAP overexpression promoted proliferation, epithelial‑mesenchymal transition (EMT) and migration/invasion of GC cells. Mechanistically, DAZAP1 physically bound NOTCH1 or JAG1 mRNA to regulate its stability. In addition, overexpression of DAZAP1 facilitated NOTCH1‑ and/or JAG1‑mediated migration via EMT in GC cells. Changes in NOTCH1 or JAG1 expression were positively correlated with DAZAP1 expression when DAZAP1 was silenced or enhanced in GC. Finally, DAZAP1 modulated the activation of the NOTCH/JAG1 signaling pathway. DAZAP1 expression facilitated migration/invasion and mediated the stabilization of NOTCH1 or JAG1 mRNA, suggesting they may participate in GC progression. - Source: PubMed
Publication date: 2026/03/06
Peng SiyangChen YidongWu JiekeHuang XiaodongHong LinjieXie YanciLei YutingWei XiangyangYang PingZhang JiemingYang QiongLiu GuangnanLi AiminLiu SideLi JiayingDai WeiyuHu YanfengWang JingXiong JingWang Jide - piRNAs (PIWI-interacting RNAs) can significantly modify the expression of protein-coding genes by suppressing the translation process. The aim of this work was to computationally evaluate the potential interactions between piRNAs and the mRNA of the gene, as well as other genes involved in key metabolic pathways related to health and lifespan regulation. - Source: PubMed
Publication date: 2026/02/18
Pyrkova AnnaAkhmetova KyrmyzyZhanuzakov MuratTauassarova MakpalRakhmetulina AizhanNiyazova RaigulOrazova SaltanatZielenkiewicz PiotrIvashchenko Anatoliy - Gastric cancer (GC) continues to be a fatal disease globally, largely due to the lack of dependable molecular indicators enabling early diagnosis and therapeutic intervention. Single-cell transcriptomic analysis revealed significant enrichment of DAZAP1 in proliferating and malignant gastric epithelial cells. Using a combined analysis of single-cell and bulk RNA-seq datasets, we further recognized DAZAP1 as a putative oncogene correlated with poor clinical outcomes in GC. Functional experiments demonstrated that DAZAP1 promotes tumor proliferation, cell cycle progression, and chemotherapy resistance in vitro and in vivo. Mechanistically, DAZAP1 bound and stabilized USP34 mRNA, leading to increased USP34 protein expression, which in turn mediated the deubiquitination and stabilization of the oncoprotein PIN1. This subsequently resulted in activation of the MAPK signaling pathway, driving GC progression and chemoresistance. Furthermore, we revealed that DAZAP1 expression is post-transcriptionally regulated by m6A modification through the demethylase ALKBH5, which protects DAZAP1 mRNA from YTHDF2-mediated degradation. Collectively, our findings establish the ALKBH5/DAZAP1/USP34/PIN1/MAPK axis as a key regulatory mechanism in gastric tumorigenesis and chemoresistance, underscoring DAZAP1 as a promising candidate for therapeutic and diagnostic applications in GC. - Source: PubMed
Publication date: 2025/12/02
Zhang PeilingMa LujuanWei YitianPeng QianXiang HongFang XishengWeng ChengyinWu YongLu Lin - Although the association between human papillomavirus (HPV) 6 and HPV11 and squamous cell carcinomas (SCCs) has been well documented, the molecular alterations and mechanisms by which these low-risk HPVs contribute to carcinogenesis remain largely unknown. In this study, we comparatively elucidated the molecular landscape of HPV6/11-associated condylomas (9 cases) and SCCs (8 cases) of the uterine cervix and vulva. Sixteen of 17 cases were successfully analyzed using deep next-generation sequencing. Recurrent molecular alterations in the SCCs included mutations in the TERT promoter (pTERT, 71%), NOTCH1 (57%), NFE2L2 (57%), NOTCH3 (29%), and CDKN2A (29%). Mutations in NOTCH1 and NFE2L2 tended to be mutually exclusive. Less common somatic mutations were each detected in the following genes: AR, ARID1A, ASXL1, BCORL1, DAZAP1, FNDC1, HRAS, KMT2B, NOTCH2, NRAS, PIK3R1, and TP53. Etiologically similar to the SCCs, the vast majority of vulvar and cervical condylomas (7/9, 78%) were infected with HPV6 rather than HPV11. Unlike the SCCs, condylomas rarely harbored recurrent pathogenic somatic mutations. NOTCH1 and NOTCH2 were the only mutant genes detected in both SCCs and condylomas in this series, suggesting a critical role for the NOTCH pathway in the initiation and maintenance of HPV6/11-related early squamous lesions. Although pathogenic mutations in NOTCH1, NOTCH2, ERBB3, ATRX, FGFR2, EPHA5, CARD11, STAG2, and TSC2 were detected in condylomas, none were recurrent, indicating diverse genetic alterations involved in the development of these lesions. Case 8 illustrated a stepwise progression from condyloma to invasive SCC, in which pathogenic mutations in pTERT and NOTCH1 were detected in the SCC (age 58) and the condyloma at age 55, but not in the condyloma at age 44. Our study presents the first report on the molecular landscape of HPV6/11-associated SCCs of the uterine cervix and vulva. We provide evidence that SCCs associated with low-risk HPV are distinct entities, differing from those related to high-risk HPV and more closely resembling HPV-independent neoplasms. Given that low-risk HPV-associated SCCs of the cervix and vulva exhibit unique morphological and molecular features, they should either be described separately within existing classification systems or classified as a distinct new entity. - Source: PubMed
Publication date: 2025/10/10
Sun YingSmith ColtonMurray JasonZhu JingPallavajjala AparnaLiang SichenKlausner MelanieTsai Ya-CheaHung Chien-FuWu Tzyy-ChoouZou Ying SXing Deyin - The quality of pig sperm is one of the crucial determinants of reproductive ability, and sperm defects can shorten the reproductive life of boars and affect the production of offspring. During transcription and translation, the gene exerts regulatory control over alternative splicing, thereby exerting influence on vital cellular processes including cell growth, development, and spermatogenesis. In this study, we employed second- and third-generation transcriptome sequencing techniques to isolate and identify the gene and its transcripts using Banna mini-pig inbred line (BMI) testicular cDNA as a template. We identified three splice variants of the gene, including ENSSSCT00000023438.4 (DAZAP1_X1), ENSSSCT00000051975.3 (DAZAP1_X2), and ENSSSCT00000074738.2 (DAZAP1_X3). Furthermore, the transcript DAZAP1_X2, was subjected to comprehensive analysis. The DAZAP1_X2 variant comprises 13 exons, with a coding sequence (CDS) length of 1254 bp (417 aa). Subsequently, enrichment analyses based on GO and KEGG pathways revealed that DAZAP1_X2 primarily participated in pathways associated with spermatogenesis, movement of the 9+2 cilium structure, germ cell development, gamete generation, and sexual reproduction. The ceRNA analysis identified three miRNAs interacting with DAZAP1_X2: ssc-miR-107, ssc-miR-127, and ssc-miR-1343, which were primarily linked to spermatogenesis. Both the testis and urethral bulb had significant levels of expression, according to multi-tissue expression analysis. Subcellular localization indicated that the DAZAP1 was mainly distributed in the cell nucleus. was critical for sperm formation and was essential for reproductive. These results shed light on the biological roles of in pigs. - Source: PubMed
Publication date: 2025/06/27
Zhang XiaHuo HailongLi HonglinLiu YongqingQiao FujieLi ChangyaoHuo Jinlong