Ask about this productRelated genes to: ISL2 antibody
- Gene:
- ISL2 NIH gene
- Name:
- ISL LIM homeobox 2
- Previous symbol:
- -
- Synonyms:
- FLJ10160
- Chromosome:
- 15q24.3
- Locus Type:
- gene with protein product
- Date approved:
- 2002-04-29
- Date modifiied:
- 2015-09-02
Related products to: ISL2 antibody
Related articles to: ISL2 antibody
- Endometriosis is a prevalent women's health disorder that lacks a definitive cure. Numerous studies have been conducted to identify the underlying causes of this disease and select the most effective pharmaceutical intervention. ISL LIM homeobox 2 (ISL2) plays a significant role in promoting angiogenesis. Contemporary investigations strongly suggest that inhibiting angiogenesis could lead to the modulation of endometriosis and reduce associated symptoms. This study aims to repurpose drugs to target ISL2 for endometriosis treatment. - Source: PubMed
Publication date: 2025/05/14
Mahdian SoodehFavaedi RahaMikaeeli GelarehMoini AshrafShahhoseini Maryam - Retinal ganglion cells (RGCs) exhibit remarkable diversity owing to their expression of developmentally expressed transcription factors. Many transcription factors are expressed in unique RGC populations, but their roles within these populations remain undiscovered. The transcription factor Islet-2 (Isl2) is expressed in approximately 30%-40% of contralateral projecting RGCs. Previous work by others found increased ipsilateral innervation of the dorsal lateral geniculate nucleus (dLGN) in Isl2 mutant mice, implicating Isl2 in promoting a contralateral RGC axon trajectory. Since Isl2 mutant mice suffer early neonatal lethality, the role of Isl2 in RGC specification has not been fully explored. To test the role of Isl2 in RGC development, two lines of retina-specific Isl2 mutant mice were generated. Contrary to the findings in Isl2 null mice, Isl2 retinal deletion does not lead to early postnatal lethality or increased ipsilateral projections to the dLGN. Instead, there is a significant reduction in the size of the dLGN and a mild reduction in the size of the ipsilateral projection to the dLGN. Retinal Isl2 mutants also exhibit diminished expression of genes characteristic of Isl2-expressing RGCs, along with increased retinal cell death during development. These findings suggest that Isl2 is required for the development and survival of Isl2-expressing RGC subtypes. - Source: PubMed
Abed SadafFeldheim David A - To generate spinal cord neurons from pluripotent stem cells via neuromesodermal progenitors (NMPs) is not only an important step for regenerative purposes but also required for human developmental research. This study describes a protocol to obtain spinal cord neurons in culture using induced pluripotent stem-cell-derived NMPs. The protocol starts with a 3D culture of NMPs and continues with the transfer of 3D NMPs to monolayer culture in which retinoic acid and sonic hedgehog pathways were triggered sequentially. The established protocol enabled generation of spinal cord neurons with active calcium signaling, electrophysiological activity, axon elongation capacity, and synaptic vesicle trafficking. The expression profile of marker proteins, including β-Tubulin, NeuroD1, Pax6, NeuN, Mnx-1, Isl1, Isl2, Map2, NF, Sox2 was detected to explore the production of developmental regulatory transcription factors and terminal differentiation markers in a time-dependent manner. Cells during differentiation process acquired a fully neural phenotype, which was confirmed by RNA sequencing at the molecular level. The protein expression profile showed neural differentiation induction pathways based on LS-MS/MS analysis. Since NMPs differentiate into spinal cord neuron cells at the developmental stage, the results of this study highlight the further potential of NMP-derived spinal cord neurons in disease modeling and treatment in the clinics. - Source: PubMed
Publication date: 2025/03/13
Şenkal-Turhan SelinayBulut-Okumuş EzgiŞahin FikrettinYavuz YavuzYılmaz BayramŞişli Hatice BurcuKalaycı SadıkÖzgün Hüseyin BuğraÖmeroğlu Ulu ZehraAkkuş Süt PınarDoğan Ayşegül - Paralog factors are considered to ensure the robustness of biological processes by providing redundant activity in cells where they are co-expressed. However, the specific contribution of each factor is frequently underestimated. In the developing spinal cord, multiple families of transcription factors successively contribute to differentiate an initially homogenous population of neural progenitors into a myriad of neuronal subsets with distinct molecular, morphological, and functional characteristics. The LIM-homeodomain transcription factors Lhx3, Lhx4, Isl1 and Isl2 promote the segregation and differentiation of spinal motor neurons and V2 interneurons. Based on their high sequence identity and their similar distribution, the Lhx3 and Lhx4 paralogs are considered to contribute similarly to these processes. However, the specific contribution of Lhx4 has never been studied. Here, we provide evidence that Lhx3 and Lhx4 are present in the same cell populations during spinal cord development. Similarly to Lhx3, Lhx4 can form multiproteic complexes with Isl1 or Isl2 and the nuclear LIM interactor NLI. Lhx4 can stimulate a V2-specific enhancer more efficiently than Lhx3 and surpasses Lhx3 in promoting the differentiation of V2a interneurons in chicken embryo electroporation experiments. Finally, Lhx4 inactivation in mice results in alterations of differentiation of the V2a subpopulation, but not of motor neuron production, suggesting that Lhx4 plays unique roles in V2a differentiation that are not compensated by the presence of Lhx3. Thus, Lhx4 could be the major LIM-HD factor involved in V2a interneuron differentiation during spinal cord development and should be considered for in vitro differentiation of spinal neuronal populations. - Source: PubMed
Publication date: 2024/07/06
Renaux EstelleBaudouin CharlotteMarchese DamienClovis YoanneLee Soo-KyungGofflot FrançoiseRezsohazy RenéClotman Frédéric - The fidelity of motor control requires the precise positional arrangement of motor pools and the establishment of synaptic connections between them. During neural development in the spinal cord, motor nerves project to specific target muscles and receive proprioceptive input from these muscles via the sensorimotor circuit. LIM-homeodomain transcription factors are known to play a crucial role in successively restricting specific motor neuronal fates. However, their exact contribution to limb-based motor pools and locomotor circuits has not been fully understood. To address this, we conducted an investigation into the role of Isl2, a LIM-homeodomain transcription factor, in motor pool organization. We found that deletion of led to the dispersion of motor pools, primarily affecting the median motor column (MMC) and lateral motor column (LMC) populations. Additionally, hindlimb motor pools lacked Etv4 expression, and we observed reduced terminal axon branching and disorganized neuromuscular junctions in -deficient mice. Furthermore, we performed transcriptomic analysis on the spinal cords of deficient mice and identified a variety of downregulated genes associated with motor neuron (MN) differentiation, axon development, and synapse organization in hindlimb motor pools. As a consequence of these disruptions, sensorimotor connectivity and hindlimb locomotion were impaired in deficient mice. Taken together, our findings highlight the critical role of in organizing motor pool position and sensorimotor circuits in hindlimb motor pools. This research provides valuable insights into the molecular mechanisms governing motor control and its potential implications for understanding motor-related disorders in humans. - Source: PubMed
Publication date: 2023/10/23
Lee YunjeongYeo In SeoKim NamheeLee Dong-KeunKim Kyung-TaiYoon JiyoungYi JawoonHong Young BinChoi Byung-OkKosodo YoichiKim DaesooPark JihwanSong Mi-Ryoung