Ask about this productRelated genes to: ELF2 antibody
- Gene:
- ELF2 NIH gene
- Name:
- E74 like ETS transcription factor 2
- Previous symbol:
- -
- Synonyms:
- EU32, NERF, NERF-2, NERF-1A, NERF-1B
- Chromosome:
- 4q31.1
- Locus Type:
- gene with protein product
- Date approved:
- 1996-04-12
- Date modifiied:
- 2016-10-05
Related products to: ELF2 antibody
Related articles to: ELF2 antibody
- The Ets transcription factors induce the expression of Tie2 and VE-cadherin. However, the regulation of these factors remains unknown. Here, we found that the gene encoding the Ets factor Elf2 was hypermethylated in Camkkβ-deficient ( ) mice. Notably, these mice exhibited suppressed expression of Elf2, Tie2, and VE-cadherin. Additionally, either the depletion of CaMKKβ in human lung endothelial cells (EC) or EC-specific deletion of in mice led to a decrease in the expression of Elf2, Tie2, and VE-cadherin. inhibition of DNA methyltransferases or EC-specific expression of wild-type CaMKKβ restored the expression of Elf2, Tie2, and VE-cadherin in mice. We find that methyl CpG binding protein MeCP2 repressed Elf2 expression. In EC-restricted knockout mice, the lung endothelial barrier was compromised due to the suppressed expression of Tie2 and VE-cadherin. Together, these findings underscore the crucial role of CaMKKβ in regulating endothelial junctional barrier integrity by controlling Elf2 expression. - Source: PubMed
Publication date: 2025/11/27
Wang Dong-MeiYang JuanAnsari Mohammad OwaisArbieva ZaremaMaienschein-Cline MarkNatarajan ViswanathanMalik Asrar BTiruppathi Chinnaswamy - The topoisomerase I inhibitor topotecan is an effective chemotherapeutic agent for retinoblastoma; however, treatment resistance remains a major clinical challenge, and its mechanisms remain elusive. Using genome-wide CRISPR-Cas9 knockout screening, we identified ELF2 as a key gene involved in topotecan resistance. Here, we show that surviving retinoblastoma cells exposed to topotecan showed progressively decreased ELF2 expression, accompanied by reduced apoptosis. In a mouse xenograft model, ELF2 disruption diminished the antitumor efficacy of topotecan, with ELF2-knockout cells exhibiting reduced topotecan-induced apoptosis. RNA sequencing further revealed that the MT-CYB pathway, associated with ATP synthesis, contributes to ELF2-mediated resistance. Importantly, clinical analysis demonstrated a correlation between ELF2 expression and tumor volume in retinoblastoma patients treated with topotecan. Together, these findings interrogate the mechanisms underlying topotecan resistance in retinoblastoma and suggest ELF2 as a potential therapeutic target to overcome drug resistance. - Source: PubMed
Publication date: 2025/12/23
Jiang JingyiJiang ZihuaLuo QianChen XiZhuang JiejieChen JiaxinMu QingqingQiu JinLi YanChen ShuxiaZhang PingYu KemingChen ShuilianLiu Guei-SheungZhuang Jing - TP53 encodes for the transcription factor p53, which binds to a diverse set of target genes in response to stress. Activation of p53 results in highly specific transcriptional responses, but the regulation of promoter selectivity by p53 is poorly understood. Here, we report that sequence-specific binding of p53 is regulated by its target gene and binding partner peptidyl-arginine deiminase 4 (PADI4). PADI4 enzymatically converts peptidyl-arginine to peptidyl citrulline in a process known as citrullination. We show that PADI4 citrullinates p53 at the C terminus in vitro, and we confirm two citrullination events in cells and mouse tissue (R306 and R363). Chromatin immunoprecipitation sequencing (ChIP-seq) reveals that PADI4 expression causes a redirection of p53 away from a subset of canonical binding sites to target genes associated with ETS transcription factors. Chromatin profiling using citrullination-specific p53 antibodies supports this conclusion. These findings link citrullination to p53 function and illustrate how chromatin modifiers like PADI4 can direct the p53 transcriptional response. - Source: PubMed
Indeglia AlexandraValdespino AndreaPantella GiuliaOffley SarahHill ConnorFoster MayaCasey KaitlynTang Hsin-YaoFaustino Anneliese MGardini AlessandroMurphy Maureen E - PCOS refers to an endocrine and metabolic disorder that affects female individuals of reproductive age. Our study explores the potential mechanism of circ_0070987 on PCOS in regulating pyroptosis of ovarian GCs, providing new evidence for PCOS treatment. PCOS cell model was established. The expression of circ_0070987, line ELF2, miR-139-5p and CDH1 was detected. Cell viability was measured. The expression of IL-1β, IL-18, NLRP3, cleaved Caspase-1, GSDMD-N, and CDH1 was analyzed. Circ_0070987 was downregulated to verify its effect on pyroptosis. The binding of miR-139-5p to circ_0070987 and CDH1 was verified by dual-luciferase report assay. The role of circ_0070987 in pyroptosis of ovarian GCs in PCOS through the miR-139-5p/CDH1 axis was validated. After DHT treatment, cell viability of SVOG was decreased, and the expression of IL-1β, IL-18, circ_0070987, NLRP3, cleaved Caspase-1 and GSDMD-N was increased. DHT-induced pyroptosis of ovarian GCs was inhibited upon circ_0070987 downregulation. Mechanistically, circ_0070987 negatively regulated miR-139-5p, which targeted and inhibited CDH1. Inhibitory effect of circ_0070987 downregulation on pyroptosis of ovarian GCs in PCOS was reduced after miR-139-5p downregulation or CDH1 overexpression. In conclusion, circ_0070987 inhibits miR-139-5p expression and upregulates CDH1 expression, thus promoting pyroptosis of ovarian GCs in PCOS. - Source: PubMed
Publication date: 2025/09/05
Huang RuiGao Miao - Copy Number Variants (CNVs) are structural variants affecting genetic diversity and phenotypic variability of populations. Different authors underlined the relevance of CNV in relation to the adaptation to environmental conditions (e.g., altitude, harsh farming environment). Aosta cattle (Aosta Red Pied - ARP; Aosta Black Pied/Chestnut - ABC and Mixed Chestnut-Héren - ACH) farmed in the Aosta Valley, and the Oropa Red Pied (ORO), farmed in the Piedmont region, are autochthonous dual-purpose breeds well adapted to the natural Alpine environment. In contrast, the Holstein (HOL) breed is a specialized dairy breed raised in intensive farming systems, representing an artificial environment. The aim of this study is to use CNVs to characterize these breeds and explore the relationship between structural genomic variability and adaptation to mountain farming systems (natural environment) vs. intensive farming systems (artificial environment). Using the GeneSeek Genomic Profiler Bovine 100K data, a total of 160,798 CNVs were identified across 5,610 individuals. Principal Component Analysis (PCA) using CNV Regions (CNVRs) revealed that Aosta breeds clustered into two separate groups, with one smaller cluster including part of ORO cows, while the Holstein formed a distinct cluster. These results suggest that CNVs may act as markers of adaptive selection, influencing both Aosta and ORO breeds, though to a different extent compared to the intensively farmed HOL breed. A total of 526 CNVRs were identified in at least 2% of the samples. Annotated genes and overlapping QTL were functionally associated with production, functional traits, and health-related characteristics. V analysis revealed candidate genes linked to environmental adaptation, reproduction, and metabolic efficiency in Aosta, ORO, and HOL cattle. Key findings include TCF12 and SRGAP1 deletions in Aosta, suggesting trade-offs between muscle growth and endurance, while ELF2 and ARID5B gains in Holstein indicate aptitude for milk protein synthesis and feed efficiency. Additionally, reproductive genes (RGS3, GSE1, MARCH10) showed distinct selection pressures between Dual-purpose and Holstein breeds, reflecting adaptation to different production systems. - Source: PubMed
Publication date: 2025/07/01
Strillacci Maria GiuseppinaBernini FrancescaVevey MarioBlanchet VeruskaBagnato Alessandro