Ask about this productRelated genes to: PAX7 antibody
- Gene:
- PAX7 NIH gene
- Name:
- paired box 7
- Previous symbol:
- -
- Synonyms:
- Hup1
- Chromosome:
- 1p36.13
- Locus Type:
- gene with protein product
- Date approved:
- 1992-11-20
- Date modifiied:
- 2014-11-18
Related products to: PAX7 antibody
Related articles to: PAX7 antibody
- Manual analysis of skeletal muscle cross-sections is time-consuming and subject to error and user bias. To overcome these limitations, we developed and validated a semi-automated, quantitative, and reproducible image-analysis pipeline specifically tailored to quantify Pax7+ satellite cells, myonuclei, and cross-sectional area by fiber type. The workflow combines Fiji/ImageJ-based image preprocessing with CellProfiler, Cellpose, and a custom Python script to process and analyze immunohistological images of muscle tissue cross-sections. Outcomes include Pax7+ satellite cells and myonuclei quantified per fiber by fiber type, along with cross-sectional area, perimeter, and fiber type classification. This semi-automated approach provides a robust and efficient platform for high-throughput analysis of muscle tissue cross-sections from large datasets. - Source: PubMed
Megowan Helia GLuu MadelineShuaib AdamAugienello Kaitlyn BFries Adam CSearcy JakeDreyer Hans C - To review the clinical research progress of craniofacial duplication, improve clinicians' recognition of this disease, and provide theoretical basis and practical references for its precise diagnosis and individualized treatment. - Source: PubMed
Li ChenxiChen YanGuo ChaoGong ZhongchengSiqin GaowaDing MingchaoLiu HuiHuang Dishu - The human body contains around 640 distinct muscles, each capable of regeneration following injury through the action of muscle-specific stem cells (MuSCs), that express the transcription factor PAX7. Its paralog, PAX3, a master regulator of embryonic myogenesis, is selectively expressed in a subset of adult quiescent MuSCs. The proportion of PAX3-pos MuSCs varies across muscles. By combining lineage tracing and skeletal muscle injury, we demonstrate that PAX3 drives MuSC diversity and muscle-specific regeneration rates. PAX3-expressing MuSCs display enhanced proliferation and differentiation capacities, enabling a faster response following injury, whereas loss of PAX3 leads to proliferation arrest and cell death. Single-cell RNA-sequencing analyses revealed the specific expression of in PAX3-pos MuSCs. We show that PAX3 is required for SIX2 expression, and loss of SIX2 in MuSCs reduces proliferation and differentiation rates. Conversely, ectopic expression promotes proliferation of PAX3-neg MuSCs by directly activating cell cycle pathways. With this work, we establish that PAX3-SIX2 expression correlates with distinct MuSCs behavior, influencing regeneration rates in a muscle-type-dependent context. Our findings highlight a previously unrecognized layer of regulation in MuSC behaviour and muscle repair and suggest that PAX3-SIX2 heterogeneity could be leveraged for targeted therapeutic strategies in muscle-wasting diseases. - Source: PubMed
Publication date: 2026/09/16
Zoglio VirginiaChebouti SarahIssa FayezMassard ThéoPolin BenjaminManin SylvieMaire PascalChoi DawonChan Indigo T CCheung Tom Hde Lima Joana EstevesRelaix Frederic - Cancer cachexia is a complex metabolic syndrome characterized by progressive skeletal muscle wasting and poor clinical outcomes. Previous studies have mainly focused on the association between cancer-derived extracellular vesicles and atrophy of differentiated myotubes, whereas their effects on myoblast differentiation markers have not been sufficiently elucidated. In this exploratory in vitro study, vesicle fractions isolated from MC38, CT26, MCA205, and B16F10 cancer cell lines under serum-deprived conditions were referred to as small extracellular vesicles (sEVs) and used to treat C2C12 myoblasts during differentiation. Compared with the control group, the sEV-treated groups showed reduced myotube diameter and differentiation area on day 6, while the mRNA expression patterns of , (), , , , and several MHC isoforms showed time-dependent changes. GO/KEGG analysis of commonly identified proteins revealed enrichment of terms related to mRNA metabolism, the cell cycle, and the proteasome. These findings suggest that treatment with cancer cell-derived sEVs may be associated with impaired in vitro differentiation of C2C12 myoblasts. - Source: PubMed
Publication date: 2026/09/04
Lee WoojinYang SeongjaeYu HyeonseoKim YeongminCho SeongminKim Hyeong YunKim UijooKong HyunseokKim Sang Bum - Rapid muscle growth suppresses intramuscular fat deposition in chickens, leading to decreased meat quality; however, the underlying mechanism remains unclear. Here, we compared differences in myogenic and adipogenic properties in the breast muscle of Ross 308 (RS) and Xueshan (XS) broilers during growth, and analyzed the contribution of hormone levels and vascular density to these differences. Our results showed that body weight, breast muscle ratio, and myofiber size were significantly greater in RS broilers from embryonic day 12 (E12), and these differences became more pronounced after day 21 (D21), which was accompanied by marked increases in the abundance of PAX7⁺ satellite cells (SCs) and enhanced expression of PAX7, MYF5, MYOD, and MYOG. In addition, higher levels of T, T, and IGF-1 were found in both yolk and serum of RS broilers from E12 to D42. In contrast, XS broilers exhibited greater intramuscular fat deposition before hatching, which was accompanied by greater number of TCF4⁺ fibro/adipogenic progenitors (FAPs) and enhanced expression of PDGFRA and ZNF423. Consistently, the number of CD31⁺ endothelial cells and expression of CD31 and VEGFA were also higher in XS broilers from E12 to D1, suggesting that enhanced angiogenesis supported FAPs proliferation. Therefore, the rapid muscle growth and enhanced myogenic capacity of SCs suppressed the proliferation and adipogenic differentiation of FAPs in broilers, with hormone levels and angiogenesis playing regulatory roles. These findings provide valuable insights into the competing mechanism of myogenesis and intramuscular adipogenesis in broilers. - Source: PubMed
Publication date: 2026/08/16
Sun XuanLiu JianliangZhang XiuzeLiu YingXing TongZhang LinGao FengZhao Liang