Ask about this productRelated genes to: NEK4 antibody
- Gene:
- NEK4 NIH gene
- Name:
- NIMA related kinase 4
- Previous symbol:
- STK2
- Synonyms:
- NRK2, pp12301
- Chromosome:
- 3p21.1
- Locus Type:
- gene with protein product
- Date approved:
- 1995-05-10
- Date modifiied:
- 2016-01-13
Related products to: NEK4 antibody
Related articles to: NEK4 antibody
- Anxiety disorders are highly heritable, but their underlying genetic mechanisms remain poorly understood. This study aimed to identify and functionally characterize genes whose expression is causally linked to anxiety disorder risk by integrating the parallel single-tissue genetic data. - Source: PubMed
Publication date: 2026/04/21
Wang LeshengXu ZhipengLuo GaomengWei WeiGuo MeimeiYuan YunheShi BeiGuan HaowenLiu ShaLi Xiang - Manual synthesis of small molecules can represent a rate-limiting step in medicinal chemistry. This study describes the application of an automated, modular synthesis platform ('Chemputer') to a drug discovery project targeting a model of KRAS-mutant colorectal cancer (K-CRC). A 4-anilinoquinazoline-based compound library was synthesized using automated and digitized protocols for nucleophilic aromatic substitution (SnAr) and Suzuki cross-coupling reactions. Chemical synthesis is guided by phenotypic screening of a transgenic Drosophila line engineered to model the genetic profile of a patient's K-CRC tumour. This integrated system enables iterative synthesis and screening cycles. An initial run identified the hit compound AP2-83, which strongly improves animal survival. Kinase profiling and genetic validation find that AP2-83 activity is mediated in part through inhibition of CLK1 and PI3K. A subsequent optimisation effort, informed by these results, produced AP4-43. AP4-43 demonstrates increased efficacy in the Drosophila model and greater potency than regorafenib in a mammalian CRC organoid growth assay. Functional analysis indicates AP4-43 acts as a multi-kinase inhibitor, with its enhanced activity associated with the inhibition of a network including CLK1 and NEK4. This work demonstrates the utility of a digital synthesis platform for generating and optimising lead compounds in a complex, preclinical drug discovery context. - Source: PubMed
Publication date: 2026/03/27
Badmos Hammed APirvan Petrisor-AlinKlimareva ElenaCronin LeeCagan Ross - Oral squamous cell carcinoma (OSCC) is a prevalent malignancy characterized by aggressive behavior, poor prognosis, and limited therapeutic options. Mutations in the NIMA-related kinase (NEK) family are increasingly implicated in tumorigenesis across various cancers. However, their contributions to OSCC pathogenesis remain largely unexplored. - Source: PubMed
Publication date: 2026/02/04
Nawab FouziaNaeem WafaFatima SadiaKhan Muhammad UzairMehmood AamirNawab SadiaKhan IshaqNawaz HaseenaAhmad HilalKhalil Ali TalhaKhan Ishtiaq AhmadIrfan MuhammadAlorini MohammedKhurram Syed AliAli Asif - Rheumatoid arthritis (RA) is a complex autoimmune disease. Recently, cell senescence has been identified as a key factor in its pathogenesis. This study integrated multi-omics summary data and applied Mendelian randomization (MR) and co-localization analysis to systematically evaluate the causal relationships between cell senescence-related genes and RA. We collected summary data on blood methylation quantitative trait loci (mQTL), expression quantitative trait loci, and protein quantitative trait loci. The FinnGen database was the primary discovery dataset, validated by the UK Biobank and GWAS Catalog. We used the summary-data-based MR method to assess causal associations between the molecular traits of cell senescence-related genes and RA. Co-localization analysis was then performed to confirm shared genetic variants. After integrating multi-omics data on cell senescence-related mQTL and expression quantitative trait loci, we identified 5 key cell senescence-related genes potentially associated with RA: BCL2L1, DNMT3B, ERRFI1, NEK4, and RAF1. These genes demonstrated significant causal associations across multiple analyses. The mQTL signals based on summary-data-based MR analysis show that the genetically regulated methylation variations at the cg12873919 (odds ratio [OR] = 0.91, 95% CI [0.84-0.99]) and cg13989999 (OR = 0.90, 95% CI [0.82-1.00]) sites of the BCL2L1 gene are negatively associated with RA risk and may mediate disease risk by upregulating gene expression (OR = 0.82, 95% CI [0.76-0.88] and OR = 0.78, 95% CI [0.71-0.87]). Conversely, the mQTL effect size at the cg26432171 site of the RAF1 (OR = 1.17, 95% CI [1.02-1.33]) is positively associated with RA risk and is consistent with the upregulation of gene expression (OR = 1.83, 95% CI [1.49-2.25]), thereby enhancing RA susceptibility. Moreover, several sites in the DNMT3B gene (e.g., cg09149842) exhibited negative correlations with RA risk, suggesting that DNMT3B may play a critical role in RA pathogenesis by affecting gene expression. Methylation sites in ERRFI1 (cg13808198, cg22678073) and NEK4 (cg09524078) were also associated with RA risk, supporting their potential regulatory roles in RA. Co-localization analysis further validated the association between methylation sites and RA, particularly for BCL2L1, RAF1, DNMT3B, ERRFI1, and NEK4, where we identified shared causal signals with RA (posterior probability of H4 > 0.5). This study systematically evaluated the causal relationships between cell senescence-related genes and RA risk. These findings provide new insights into RA pathogenesis and reinforce the clinical value of these genes as potential therapeutic targets. - Source: PubMed
Jiang PingJia YoujiZhang JuhuaWang ZhiMa HonghongGuo YajuanWang MingcongYan WeiXi Xiaobing - Meiosis is a conserved yet evolutionarily varied process underpinning sexual reproduction in eukaryotes. In the malaria parasite , meiosis is unconventional: it occurs immediately after fertilisation (post-zygotic) and must be coordinated with the transformation of the zygote into a motile ookinete. The mechanisms synchronising these meiotic and morphogenetic programmes remain unknow. Here, we identify the NIMA-related kinase, NEK4 as a key regulator that couples meiotic initiation with zygote morphogenesis. Using ultrastructure expansion microscopy, we show that NEK4 accumulates at the microtubule-organising centre (MTOC) and the apical polar complex (APC) shortly after fertilisation, preceding the assembly of perinuclear and cortical microtubules. We reveal that zygotes undergo a nuclear migration driven by the MTOC, analogous to the meiotic nuclear movement in fission yeast. Deletion of results in complete developmental arrest: MTOC duplication and microtubule formation are blocked, chromatin remains uncondensed, and nuclear migration and cell polarity fail to establish. Transcriptomic and phosphoproteomic analyses reveal that NEK4 absence causes a collapse in transcriptional and phosphoregulatory networks governing meiosis and cytoskeletal organisation, leading to reduced expression and phosphorylation of important players, including HOP1, REC8, and AP2-O. These findings establish NEK4 as a key regulator driving meiotic entry and zygote maturation. - Source: PubMed
Publication date: 2025/12/09
Yanase RyujiHair MollyZeeshan MohammadFerguson David J PBrady DeclanPasquarello CarlaBottrill AndrewBhanvadia SuhaniNeal ArrmundTromer Eelco CLe Roch Karine GHainard AlexandreHolder Anthony AVaughan SueGuttery David STewari Rita