Ask about this productRelated genes to: TMPRSS12 antibody
- Gene:
- TMPRSS12 NIH gene
- Name:
- transmembrane serine protease 12
- Previous symbol:
- -
- Synonyms:
- MGC57341, CT151
- Chromosome:
- 12q13.12
- Locus Type:
- gene with protein product
- Date approved:
- 2005-02-24
- Date modifiied:
- 2018-01-19
Related products to: TMPRSS12 antibody
Related articles to: TMPRSS12 antibody
- Ovarian cancer metastasizes via peritoneal dissemination, requiring tumor cells to resist detachment-induced cell death (anoikis) in suspension and to reinitiate proliferation after seeding. Because disseminating ovarian cancer cells persist in malignant ascites enriched in extracellular S1 family serine proteases, we surveyed S1 protease gene expression across ovarian cancer cohorts to identify those most associated with poor outcomes. High expression of multiple family members was associated with poor survival, with HTRA3, TMPRSS12, and PRSS23 among the strongest hits. TMPRSS12 transcripts were below the limit of detection in our cell line panel, while HTRA3 depletion showed modest, histotype-dependent effects. In contrast, PRSS23 knockdown reduced proliferation and increased anoikis sensitivity in high-grade serous (HGSOC) and clear cell (OCCC) ovarian carcinoma cell lines, and diminished tumor establishment, dissemination, and ascites in intraperitoneal xenograft models. RNA-seq of PRSS23-depleted cells revealed a conserved program of reduced cell-cycle/DNA repair gene expression with induction of inflammatory and adhesion/epithelial-mesenchymal transition pathways. Endogenous epitope tagging demonstrated that PRSS23 is synthesized as a precursor and secreted as a processed, glycosylated protease homology domain that retains the catalytic triad, yet lacks the canonical Ile16-Asp194 zymogen activation switch. In complementary biochemical assays, PRSS23 showed no detectable serine hydrolase activity in either activity-based probe labeling of conditioned media or chromogenic peptide substrate assays using the recombinant protease domain. Furthermore, pro-tumorigenic phenotypes persisted after mutation of the putative catalytic serine. Together, these findings demonstrate protease-independent PRSS23 function in ovarian cancer peritoneal dissemination, and suggest that PRSS23 may ultimately warrant reclassification as a serine pseudoprotease. - Source: PubMed
Publication date: 2026/04/13
Akhtar SharoonCoban MattMiller ErinHockla AlexandraMaina EranMehner ChristineIlic StefanPapo NivRadisky Derek CRadisky Evette S - The development of non-hormonal male contraceptives requires validated preclinical models. This study investigated whether human orthologs of two mouse testis-specific serine proteases, PRSS55 and TMPRSS12, both essential for male fertility in mice, could functionally rescue the infertility phenotypes of their respective knockout mouse lines. We generated transgenic mouse lines expressing human PRSS55 with either an extracellularly or intracellularly positioned C-terminal 3xFLAG tag (RES GPI or RES TM), and a line expressing human TMPRSS12 with a C-terminal 3xFLAG tag (T12 RES), all on their respective mouse null backgrounds. Fertility was assessed through continuous mating trials, and sperm parameters were evaluated. Both hPRSS55 rescue lines sired offspring; RES GPI males exhibited partially restored fertility with significantly fewer pups per litter compared to controls, while RES TM males demonstrated fertility comparable to controls. In contrast, T12 RES males remained infertile, exhibiting severe defects in sperm motility and other parameters, despite confirmed transgene mRNA expression. These findings indicate that human PRSS55, particularly when tagged intracellularly, can functionally replace its mouse counterpart, validating the RES TM line as a promising model for testing human PRSS55-targeted contraceptives. The inability of hTMPRSS12 to rescue fertility highlights challenges potentially due to sequence divergence or unconfirmed protein expression levels. - Source: PubMed
Publication date: 2025/08/05
Sutton Courtney MUmezu KoheiMashiko DaisukeIkawa MasahitoLarina Irina VGarcia Thomas XMatzuk Martin M - Colon cancer (CC) is a significant cause of death worldwide, particularly in Saudi Arabia. To increase the accuracy of diagnosis and treatment, it is important to discover new specific biomarkers for CC. The main objectives of this research are to identify potential specific biomarkers for the early diagnosis of CC by analyzing the expressions of eight cancer testis (CT) genes, as well as to analyze how epigenetic mechanisms control the expression of these genes in CC cell lines. Tissue samples were collected from 15 male patients with CC tissues and matched NC tissues for gene expression analysis. The expression levels of specific CT genes, including ADAD1, DMRTC2, PRSS54, SYCE1, SYCP1, TEX101, TEX48, and TMPRSS12, were assessed using quantitative techniques. To validate the gene expression patterns, we used publicly available CC statistics. To investigate the effect of inhibition of DNA methylation and histone deacetylation on CT gene expression, in vitro experiments were performed using HCT116 and Caco-2 cell lines. There was no detected expression of the genes neither in the patient samples nor in NC tissues, except for TEX48, which exhibited upregulation in CC samples compared to NC tissues in online datasets. Notably, CT genes showed expression in testis samples. In vitro, experiments demonstrated significant enhancement in mRNA expression levels of ADAD1, DMRTC2, PRSS54, SYCE1, SYCP1, TEX101, TEX48, and TMPRSS12 following treatment with 5-aza-2'-deoxycytidine and trichostatin A in HCT116 and Caco-2 cell lines. Epigenetic treatments modify the expression of CT genes, indicating that these genes can potentially be used as biomarkers for CC. The importance of conducting further research to understand and target epigenetic mechanisms to improve CC treatment cannot be overemphasized. - Source: PubMed
Publication date: 2024/08/29
Almutairi Mikhlid HAlrubie Turki MAlshareeda Alaa TAlbarakati NadaAlmotiri AlhomidiAlamri Abdullah MAlmutairi Bader OAlanazi Mohammad - Serine proteases are involved in many physiological activities as initiators of proteolytic cascades, and some members have been reported to play roles in male reproduction. Transmembrane serine protease 12 (TMPRSS12) has been shown to regulate sperm motility and uterotubal junction migration in mice, but its role in the testis remains unknown. In this study, we verified that TMPRSS12 was expressed in the spermatocytes and spermatids of testis and the acrosome of sperm. Mice deficient in exhibited male sterility. In meiosis, TMPRSS12 was demonstrated to regulate synapsis and double-strand break repair; spermatocytes of mice underwent impaired meiosis and subsequent apoptosis, resulting in reduced sperm counts. During spermiogenesis, TMPRSS12 was found to function in the development of mitochondria; abnormal mitochondrial structure in sperm led to reduced availability of ATP, impacting sperm motility. The differential protein expression profiles of testes in and wild-type mice and further molecule identification revealed potential targets of TMPRSS12 related to meiosis and mitochondrial function. Besides, TMPRSS12 was also found to be involved in a series of sperm functions, including capacitation, acrosome reaction and sperm-egg interaction. These data imply that TMPRSS12 plays a role in multiple aspects of male reproduction. - Source: PubMed
Publication date: 2022/04/25
Zhang JingjingZhou XinliWan DanyangYu LiChen XuYan TongWu ZhuZheng MeimeiZhu FengZhu Hui - As COVID-19 infections wreak havoc across the globe, attention has rightly been focused on the vital organ systems (lung, kidney and heart) that are vulnerable to viral attack and contribute to the acute pathology associated with this disease. However, we should not lose sight of the fact that COVID-19 will attack any cell type in the body expressing ACE2 - including human spermatozoa. These cells possess the entire repertoire of receptors (AT1R, AT2R, MAS) and ligand processing enzymes (ACE1 and ACE2) needed to support the angiotensin signalling cascade. The latter not only provides COVID-19 with a foothold on the sperm surface but may also promote integration, given the additional presence of a range of proteases (TMPRSS2, TMPRSS11B, TMPRSS12, furin) capable of promoting viral fusion. This article reviews the roles played by these various cellular constituents in maintaining the vitality of human spermatozoa and their competence for fertilization. The reproductive consequences of a viral attack on these systems, in terms of fertility and the risk of sexual transmission, are currently unknown. However, we should be alive to the possibility that there may be reproductive consequences of COVID-19 infection in young males that go beyond their capacity to survive a viral attack. - Source: PubMed
Publication date: 2020/08/05
Aitken Robert John