Ask about this productRelated genes to: SMPD3 antibody
- Gene:
- SMPD3 NIH gene
- Name:
- sphingomyelin phosphodiesterase 3
- Previous symbol:
- -
- Synonyms:
- NSMASE2
- Chromosome:
- 16q22.1
- Locus Type:
- gene with protein product
- Date approved:
- 2000-12-12
- Date modifiied:
- 2015-12-10
Related products to: SMPD3 antibody
Related articles to: SMPD3 antibody
- Extracellular vesicles (EVs) are nano-sized, membrane-delimited, particles released by cells that carry signaling macromolecules. A major pathway of EV production is potentiated by neutral sphingomyelinase 2 (SMPD3/nSMAse2), an enzyme that generates ceramide from sphingomyelin. In our attempt to study this pathway in adipocytes of male mice, we discover that the elimination of SMPD3 from adipocytes in vivo triggers a signal to surrounding immune cell-like preadipocytes to release EVs that carry SMPD3 mRNA. This results in a widespread increase in SMPD3 mRNA in purified null adipocytes without a change in the transcripts of other enzymes involved in ceramide metabolism. These results point to a selective mechanism by which specific mRNA molecules are acquired from the microenvironment to a level that can restore expression of mRNA and protein in a cell that is depleted of the corresponding genetic information. - Source: PubMed
Publication date: 2026/04/11
Crewe ClairGliniak Christy MOnodera ToshiharuChen ShiuhweiFuncke Jan-BerndWang May-YunTiash SnigdhaPai Yun-LingRusso MarjoriPatel Saket AwadhesbhaiYu ZeZheng Yi-CianLarkin AlexXing ChaoGautron LaurentChen Chun-KanLiu ChenScherer Philipp E - The choroid plexus (CP) in the brain ventricles secretes cerebrospinal fluid (CSF) that bathes the adjacent subventricular zone (SVZ). As the largest adult neurogenic region enriched with neural stem/progenitor cells (NSPCs), the SVZ supplies newborn neurons to the olfactory bulb (OB) for normal olfaction. This report depicts the presence of a CP-SVZ regulatory (CSR) axis, in which the CP regulates SVZ adult neurogenesis and olfaction via secretion of small extracellular vesicles (sEVs). The proposed CSR axis was supported by the evidence of (1) a direct effect of CP epithelial cells on the SVZ by in vivo transplantation and in vitro CP-SVZ co-culture assays, (2) differential OB neurogenesis following intracerebroventricular (ICV) infusion of sEVs derived from the CP of control or manganese (Mn)-poisoned mice, (3) progressively diminished SVZ adult neurogenesis after CP-selective inhibition of sEV secretion via AAV5-mediated SMPD3 knockdown, and (4) compromised olfactory performance following CP-selective SMPD3-knockdown. Collectively, our findings demonstrate the physiological, toxicological, and behavioral importance of this sEV-dependent CSR axis in the adult brain. - Source: PubMed
Publication date: 2026/03/31
Liu Luke LShannahan JonathanZheng Wei - Huntington's disease (HD) is a devastating neurodegenerative disorder caused by mutant huntingtin protein, leading to progressive motor, cognitive, and behavioral decline. This study investigates the dysregulation of sphingomyelin (SM) metabolism, the most abundant sphingolipid in cellular membranes, in the striatum of R6/2 and zQ175 HD mouse models. Our findings reveal significant alterations in the expression of SM-metabolizing enzymes and a concomitant accumulation of SM in symptomatic HD mice. Notably, transcriptional dysregulations were detectable at early and even pre-symptomatic stages of the disease, suggesting an active role in disease initiation or early progression. Moreover, administration of THI, a sphingolipid modulator, normalized SM levels in R6/2 mice and led to a selective elevation in the expression of the sphingomyelinase gene Smpd3. These results reinforce the concept that sphingolipid metabolism is a critical and druggable pathway in HD, offering a promising target for novel therapeutic interventions. - Source: PubMed
Publication date: 2026/03/18
Pizzati LudovicaPepe GiuseppeValentini NicoleNoro FabriziaRealini NataliaScarselli PamelaCerletti ChiaraStorto MariannaArmirotti AndreaDi Pardo AlbaMaglione Vittorio - Sirtuin 1 (Sirt1), a member of the sirtuin family, is integral to the regulation of energy homeostasis, cellular metabolism, and stress responses. While Sirt1 has been intensively studied in mammals, studies on this gene in aquatic animals, especially turbot, is relatively limited. In this study, the sirt1 gene was cloned. The open reading frame (ORF) of the Sirt1 consists of 2187 base pairs, encoding a 728-amino-acid protein that contains a SIR2 domain. Compared with the siRNA-NC group, Sirt1 knockdown resulted in a significant downregulation of mRNA expression levels of the tight junction proteins occludin, tricellulin, claudin3, and zo1, as well as protein levels of Occludin and ZO1, within the intestinal tissue of turbot. Concurrently, it markedly inhibited the expression of genes associated with ceramide synthesis (sptlc2, kdsr, cers1, cers2, cers3, smpdl3a, smpdl3b, neu1, glb1, gba1, and sgpp2) and ceramide catabolism (sgms1a, ugcg, b4galt, and sphk1) in the same tissue. Conversely, compared to the pcDNA3.1 group, Sirt1 overexpression significantly enhanced the mRNA expression levels of occludin, tricellulin, claudin3, claudin7, and zo1, along with the protein level of Occludin. Furthermore, Sirt1 overexpression significantly elevated the expression of genes involved in ceramide synthesis (cers2, cers3, smpd3, smpdl3b, neu1, glb1, gba1, sgpp2) and ceramide catabolism (sgms1a, galt, b4galt, and sphk1). These results suggest that Sirt1 may influence the intestinal mechanical barrier by acting on the metabolic balance of ceramide and altering the expression of intestinal tight junction proteins, thus playing a crucial role in maintaining the intestinal health of turbot. - Source: PubMed
Publication date: 2026/03/18
Ma XiuhuaLiu QianhuiMai KangsenZhang Yanjiao - Rosemary-derived triterpene acids (TAs), primarily composed of ursolic acid, oleanolic acid, and betulinic acid, exhibit multiple bioactive properties. However, their effects on lipid metabolism and the underlying regulatory pathways remain unclear. This study investigated the effects of dietary supplementation with TAs on the growth performance, digestive and absorptive function, and hepatic lipid metabolism in juvenile grass carp (Ctenopharyngodon idella). - Source: PubMed
Publication date: 2026/03/12
Wu ZixuanZhou XiaoqiuFeng LinWu PeiZhang HongyunMa YaobinLiu YangWu CaimeiTang JiayongZhang RuinanJiang Weidan