Ask about this productRelated genes to: CHST8 antibody
- Gene:
- CHST8 NIH gene
- Name:
- carbohydrate sulfotransferase 8
- Previous symbol:
- -
- Synonyms:
- GALNAC-4-ST1
- Chromosome:
- 19q13.11
- Locus Type:
- gene with protein product
- Date approved:
- 2001-06-27
- Date modifiied:
- 2016-10-05
Related products to: CHST8 antibody
Related articles to: CHST8 antibody
- Mammalian proteins are decorated with a variety of glycans, providing proteins with enormous functional diversity. GalNAcβ1-4GlcNAc (LacdiNAc or LDN), a unique sub-terminal glycan structure regulating the half-life of circulating glycoproteins, is biosynthesized by the dedicated glycosyltransferases, B4GALNT3 and B4GALNT4. We recently reported that B4GALNT3 contains a unique non-catalytic PA14 domain that is necessary for the enzyme activity, while the precise function of PA14 is unclear. Here we show that PA14 in B4GALNT3 is a lectin domain required for the activity of B4GALNT3 toward glycoprotein substrates. Glycan microarray experiments, together with surface plasmon resonance and molecular dynamics simulations, demonstrated the specific binding between the PA14 domain of B4GALNT3 and sulfated glycan ligands, such as Gal[6S]β1-4GlcNAc[6S]. Both addition of the sulfated disaccharide ligands and point-mutation at the putative sugar binding site in the PA14 domain inhibited the in vitro activity of B4GALNT3 particularly toward glycoprotein substrates. These data suggest that sulfated glycans negatively regulate the PA14-dependent catalytic activity of B4GALNT3 toward glycoproteins. Suppression of cellular glycan sulfation by knocking out the Golgi transporters for sulfation donor PAPS, SLC35B2, and SLC35B3, likely resulted in the enhanced biosynthesis of LDN by B4GALNT3 in cells. Moreover, overexpression of CHST8, which catalyzes sulfation of LDN, seemed to reduce B4GALNT3 activity in cells, suggesting negative feedback regulation of B4GALNT3 by the sulfated product. These findings indicate that recognition of sulfated glycan ligands by its PA14 domain negatively regulates B4GALNT3 activity, highlighting a novel regulation mechanism for LDN synthesis mediated by a lectin domain. - Source: PubMed
Publication date: 2026/02/26
Tokoro YukoYamasaki TakahiroTateno HiroakiKamatari Yuji OSepehri BakhtyarSensui TomohiroKawashima HirotoDoerksen Robert JKizuka Yasuhiko - Carbohydrate sulfotransferase 8 (CHST8) promotes immune checkpoint activation by sulfating GalNAcβ1-4GlcNAc (LacdiNAc) termini on glycoproteins. We present a protocol for quantifying LacdiNAc levels in mammalian cells using a lectin-captured luciferase reporter assay. We describe steps for preparing plasmid encoding NanoLuc-fused protein and lectin-coated multiple well plates, expressing NanoLuc-fused protein, and detecting luciferase activity. We then detail procedures for the lectin-capture assay and analyzing data after luminescence signals are measured. This protocol is adaptable for other glycoforms by substituting appropriate lectins. For complete details on the use and execution of this protocol, please refer to Chou et al.. - Source: PubMed
Publication date: 2025/06/06
Chou Wen-ChengChen Wei-TingJou Yuh-ShanShen Chen-Yang - Immune checkpoint blockade (ICB) is a promising therapy for solid tumors, but its effectiveness depends on biomarkers that are not precise. Here, we utilized genome-wide association study to investigate the association between genetic variants and tumor mutation burden to interpret ICB response. We identified 16 variants (p < 5 × 10) probed to 17 genes on 9 chromosomes. Subsequent analysis of one of the most significant loci in 19q13.11 suggested that the rs111308825 locus at the enhancer is causal, as its A allele impairs KLF2 binding, leading to lower carbohydrate sulfotransferase 8 (CHST8) expression. Breast cancer cells expressing CHST8 suppress T cell activation, and Chst8 loss attenuates tumor growth in a syngeneic mouse model. Further investigation revealed that programmed death-ligand 1 (PD-L1) and its homologs could be sulfated by CHST8, resulting in M2-like macrophage enrichment in the tumor microenvironment. Finally, we confirmed that low-CHST8 tumors have better ICB response, supporting the genetic effect and clinical value of rs111308825 for ICB efficacy prediction. - Source: PubMed
Publication date: 2023/12/30
Chou Wen-ChengChen Wei-TingKuo Chun-TseChang Yao-MingLu Yen-ShenLi Chia-WeiHung Mien-ChieShen Chen-Yang - Genome-wide association studies (GWAS) are one of the best ways to look into the connection between single-nucleotide polymorphisms (SNPs) and the phenotypic performance. This study aimed to identify the genetic variants that significantly affect the important reproduction traits in Vrindavani cattle using genome-wide SNP chip array data. In this study, 96 randomly chosen Vrindavani cows were genotyped using the Illumina Bovine50K BeadChip platform. A linear regression model of the genome-wide association study was fitted in the PLINK program between genome-wide SNP markers and reproduction traits, including age at first calving (AFC), inter-calving period (ICP), dry days (DD), and service period (SP) across the first three lactations. Information on different QTLs and genes, overlapping or adjacent to genomic coordinates of significant SNPs, was also mined from relevant databases in order to identify the biological pathways associated with reproductive traits in bovine. The Bonferroni correction resulted in total 39 SNP markers present on different chromosomes being identified that significantly affected the variation in AFC (6 SNPs), ICP (7 SNPs), DD (9 SNPs), and SP (17 SNPs). Novel potential candidate genes associated with reproductive traits that were identified using the GWAS methodology included UMPS, ITGB5, ADAM2, UPK1B, TEX55, bta-mir-708, TMPO, TDRD5, MAPRE2, PTER, AP3B1, DPP8, PLAT, TXN2, NDUFAF1, TGFA, DTNA, RSU1, KCNQ1, ADAM32, and CHST8. The significant SNPs and genes associated with the reproductive traits and the enriched genes may be exploited as candidate biomarkers in animal improvement programs, especially for improved reproduction performance in bovines. - Source: PubMed
Publication date: 2023/11/04
Gangwar MunishKumar SubodhAhmad Sheikh FirdousSingh AkanshaAgrawal SwatiAnitta P LKumar Amit - A genome-wide association study (GWAS) of the daughter pregnancy rate (DPR), cow conception rate (CCR), and heifer conception rate (HCR) using 1,001,374-1,194,736 first-lactation Holstein cows and 75,140-75,295 SNPs identified 7567, 3798, and 726 additive effects, as well as 22, 27, and 25 dominance effects for DPR, CCR, and HCR, respectively, with log(1/p) > 8. Most of these effects were new effects, and some new effects were in or near genes known to affect reproduction including , , and , and a gene cluster of pregnancy-associated glycoproteins. The confirmed effects included those in or near the and regions of Chr06 and the region of Chr01. Eleven SNPs in the region of Chr18, the region of Chr06, and the region of Chr01 with sharply negative allelic effects and dominance values for the recessive homozygous genotypes were recommended for heifer culling. Two SNPs in and near the region of Chr04 that were sharply negative for HCR and age at first calving, but slightly positive for the yield traits could also be considered for heifer culling. The results from this study provided new evidence and understanding about the genetic variants and genome regions affecting the three fertility traits in U.S. Holstein cows. - Source: PubMed
Publication date: 2023/06/22
Liang ZuoxiangPrakapenka DzianisVanRaden Paul MJiang JicaiMa LiDa Yang