Ask about this productRelated genes to: TMEM146 antibody
- Gene:
- CATSPERD NIH gene
- Name:
- cation channel sperm associated auxiliary subunit delta
- Previous symbol:
- TMEM146
- Synonyms:
- MGC39581
- Chromosome:
- 19p13.3
- Locus Type:
- gene with protein product
- Date approved:
- 2006-03-30
- Date modifiied:
- 2016-02-04
Related products to: TMEM146 antibody
Related articles to: TMEM146 antibody
- The success of spermatogenesis depends on the interplay of various biomolecules that ultimately determine sperm quality. In this study, RNA-seq analysis of frozen-thawed buffalo sperm (n = 8) revealed the presence of 263 mineral-associated genes (>1 FPKM) in high (n = 4) and 181 in low motile (n = 4) sperm groups. Among these, 177 mineral-associated genes were commonly expressed between them, and the majority were upregulated (>1 fold), LOC102391588 (ncRNA; 37-fold), ZNF699 (26.5-fold), MYZAP (13-fold), etc., in the high motile group. The expression of selected mineral-associated genes was validated. The top enriched functions in commonly expressed genes were regulation of transcription by RNA polymerase II (FDR: 4.7 × 10⁻; ZNF331, ZNF692, ZNF180, etc.), followed by spermatogenesis (FDR: 2.9 × 10⁻; CALR3, ADAM18, ADAM9, etc.), proton transmembrane transport (FDR: 4.0 × 10⁻; ATP6V0E1, ATP1A4, ATP6V1B2, etc.) and flagellated sperm motility (FDR: 2.3 × 10⁻; CATSPERD, EFCAB6, CABS1, etc.). Additionally, the chromatin remodeling pathway (FDR: 3 × 10⁻; PTP4A1, PPM1A, DUSP1, etc.) emerged as the most significant and may suggest that these minerals influence genome packaging and sperm functionality. Mineral-associated genes were predominantly associated with zinc (49%), followed by calcium (20%), phosphorus (10%), iron (5%), sodium (2%), potassium (2%), copper (1%) and other trace elements (11%). Although the current study uses frozen-thawed sperm, the findings indicate that mineral-associated genes are crucial for promoting membrane stability, energy production, motility and chromatin integrity, which may contribute to the superior fertilizing ability of sperm. - Source: PubMed
Publication date: 2026/08/14
Manjunatha A TShalini RBinsila B KKrishnappa BRajendran DAthira TParvathi KSahoo ASelvaraju S - Mammalian sperm RNAs, encompassing coding and non-coding sequences, have historically been considered residual by-products of spermatogenesis. However, accumulating evidence has revealed that mature sperm-derived RNAs not only participate in zygote formation and embryonic cleavage but also exhibit multifaceted biological functions. Notably, interspecies variations in sperm transcriptomic profiles among cattle, sheep, and goats, particularly those linked to species-specific regulatory networks with fertilization-associated genes, remain underexplored. This study systematically characterized mRNAs, miRNAs, and circRNAs components within sperm transcriptomes across these three ruminant species, with comparative analysis of interspecies divergence. High-throughput sequencing identified distinct RNA repertoires: cattle sperm contained 15,661 mRNAs, 442 miRNAs, and 3,358 circRNAs; sheep sperm comprised 13,883 mRNAs, 474 miRNAs, and 3,449 circRNAs; goat sperm exhibited 15,725 mRNAs, 588 miRNAs, and 5,081 circRNAs. Cross-species comparisons revealed that 5,782 differentially expressed mRNAs, 11 differentially expressed miRNAs, and 60 differentially expressed circRNAs were shared by the three species, alongside species-unique gene clusters. A competitive endogenous RNAs (ceRNA) network integrating mRNAs, miRNAs, and circRNAs was constructed, and species-specific regulatory networks associated with fertilization were identified, including circMEMOl-bta-miR-497-IZUMO4, circAKT2-novel-bta-miR433-3p-CATSPERD, circFBXW7-novel-bta-miR382-3p-CATSPER2, circSTAU1-novel-oar-miR1301-5p-PRM3, and circJMJD1C-novel-chi-miR2159-3p-IZUMO2. This study demonstrates the interspecies divergences in sperm transcriptomic profiles and regulatory networks among cattle, sheep, and goats, establishing a novel research paradigm centered on 'coding & non-coding RNAs interplay' in mammalian sperm transcriptomic regulation, and thereby providing a generalizable systematic analytical tool for deciphering complex reproductive biological processes. - Source: PubMed
Publication date: 2026/07/24
Zhao YueBai LinfengJiang QiqiXiao HaoLiu FangyuanWuyun BiligeWu RihanHao ChunxiaDing RuiYuan PengWang LizhiLu YingSun QingyuanWang ZhigangSong YongliLi Xihe - Objective of this study is to conduct a genome-wide association study (GWAS) of first-parity reproductive traits in Suzi pigs to identify significant single-nucleotide polymorphisms (SNPs) or candidate genes influencing these traits. - Source: PubMed
Publication date: 2025/11/06
Fu YanfengLi WeiningDai ChaohuiLiao ChaoCheng JinhuaLi HuiZhao Weimin - Extracellular matrices surrounding eggs in fish (chorion) and mammals (zona pellucida [ZP]) regulate gamete recognition before fertilization, though their mechanisms differ. Mouse sperm bind and cross the ZP at any site, while fish sperm cross the chorion through a funnel-shaped opening, the micropyle. To explore these divergent processes, we established cross-species insemination assays, mixing zebrafish eggs with mouse sperm. While mouse sperm could not bind to the chorion, a subpopulation successfully located and crossed the fish micropyle. Confocal and electron microscopy revealed that sperm entered the micropyle and accumulated in the zebrafish inter-chorion space. However, transgenic mouse sperm with mCherry-labeled acrosomes failed to undergo acrosome exocytosis efficiently in the micropyle, with both acrosome-intact and reacted sperm found in the inter-chorion space. Sperm entry and crossing were dependent on hyperactive motility, as sperm from mice, which fail to undergo hyperactivation, did not interact with or cross the micropyle. These findings suggest a conserved mechanism for sperm entry into the micropyle, providing a novel platform to investigate cross-species gamete interactions and uncover novel steps in fertilization. - Source: PubMed
Publication date: 2025/10/16
Garibova SumaStickler EvaAlAli FatimaAbdulla Maha ASathappan AbbiramiDa'as Sahar IGhanem LillianDjekidel Mohamed NadhirPortman RickAvella Matteo - Circular RNAs (circRNAs) are a class of noncoding RNAs with a covalently closed loop. Studies have demonstrated that circRNA can function as microRNA (miRNA) sponges or competing endogenous RNAs. Although circRNA has been explored in some species and tissues, the genetic basis of testis development and spermatogenesis in donkeys remain unknown. We performed RNA-seq to detect circRNA expression profiles of adult donkey testes. Length distribution and other characteristics were shown a total of 1971 circRNAs were differentially expressed and 12,648 and 6261 circRNAs were detected from the testis and caput epididymis, respectively. Among these circRNAs, 1472 circRNAs were downregulated and 499 circRNAs were upregulated in the testis. Moreover, KEGG pathway analyses and Gene Ontology were performed for host genes of circRNAs. A total of 39 upregulated circRNA host genes were annotated in spermatogenesis terms, including , , , , and . Other host genes were annotated in the focal adhesion, Rap1 signaling pathway. Downregulated expressed circRNA host genes participated in the TGF-β signaling pathway, GnRH signaling pathway, estrogen signaling pathway, and calcium signaling pathway. Our discoveries provide a solid foundation for identifying and characterizing critical circRNAs involved in testis development or spermatogenesis. - Source: PubMed
Publication date: 2022/12/25
Sun YanWang YonghuiLi YuhuaAkhtar FaheemWang ChangfaZhang Qin