Ask about this productRelated genes to: TMEM16A antibody
- Gene:
- ANO1 NIH gene
- Name:
- anoctamin 1
- Previous symbol:
- ORAOV2, TMEM16A
- Synonyms:
- TAOS2, FLJ10261, DOG1
- Chromosome:
- 11q13.3
- Locus Type:
- gene with protein product
- Date approved:
- 2003-10-16
- Date modifiied:
- 2016-10-05
Related products to: TMEM16A antibody
Related articles to: TMEM16A antibody
- Hypertrophic cardiomyopathy (HCM) is one of the most common genetic cardiovascular conditions, which may be linked to ion channel dysfunction. In decades, Genetic studies in HCM patients have shown that multiple gene mutations in potassium and calcium ion channels, which may contribute to HCM progression. The direct relationship between HCM and ion channels still needs to be clarified. - Source: PubMed
Publication date: 2026/05/04
Li ShaohuaXu JingqiZhang FengXu QingLao DonghuiZhang TianyuYe YanrongTian Yuan - Colorectal cancer (CRC) metastasis remains a clinically intractable challenge, where the partial epithelial-mesenchymal transition (partial EMT) state is a key driver of metastatic plasticity and treatment resistance. The calcium-activated chloride channel ANO1 (TMEM16A) is frequently overexpressed in epithelial tumors, yet its mechanism in regulating metastasis and partial EMT remains undefined. In this study, we identify ANO1 as a key stabilizer of the partial EMT state. ANO1 was upregulated in CRC tissues and correlated with poor patient survival. Functionally, ANO1 knockdown robustly suppressed CRC cell proliferation, migration, and invasion, and potently induced early apoptosis. Proteomic profiling linked ANO1 depletion to the downregulation of cytoskeletal and adhesion networks. Given the centrality of these processes to EMT, we specifically examined key EMT-associated molecules. Western blot results showed that ANO1 silencing reduced expression of mesenchymal markers (N-cadherin, vimentin, α-SMA) and EMT transcription factors (Zeb1, Snail, Twist), while also downregulating the epithelial marker E-cadherin. Immunofluorescence revealed that ANO1 depletion disrupted a spatially organized hybrid subcellular architecture, and flow cytometry demonstrated that ANO1 knockdown selectively depleted the E-cadherin/vimentin partial EMT subpopulation. Collectively, these data indicated that ANO1 depletion actively dismantles the organized architecture of the partial EMT state rather than driving a canonical phenotypic reversal. Transcriptomic and integrated multi‑omics analyses further revealed that ANO1 loss induced widespread uncoupling between transcriptional and proteomic regulation, with the mTOR and MAPK pathways enriched among "transcript‑only" changes. Consistently, ANO1 silencing reduced phosphorylation of PI3K, AKT, mTOR, and ERK without altering total protein levels, and pharmacological inhibition of either pathway phenocopied the dissolution of the partial EMT state. In summary, our work delineates a novel mechanism wherein ANO1, acting as an upstream hub, licenses metastatic progression by co‑activating PI3K/AKT/mTOR and ERK signaling to stabilize a plastic, survival‑competent partial EMT state, thereby nominating it as a promising therapeutic target for intercepting CRC dissemination. - Source: PubMed
Publication date: 2026/05/17
Wu FeiZhang KunDu ZhenwuLi XinYang LongfeiZhao LinxianCheng BoNie LiliYang QiweiSui Yujie - Gastric schwannomas are rare, benign gastrointestinal mesenchymal tumors that pose diagnostic challenges because they overlap histologically with other mesenchymal neoplasms, particularly gastrointestinal stromal tumors (GISTs). Thus, reliable diagnostic criteria are critical for distinguishing gastric schwannomas from GISTs to ensure timely, appropriate therapy. We conducted a retrospective study of patients diagnosed with gastric schwannoma at 2 institutions to evaluate the pathological features of gastric schwannomas and their distinguishing characteristics. Patient demographics, radiologic, histopathologic, and immunohistochemical features were analyzed. A total of 22 patients with a median age of 66 years (range, 36-77) were included: 7 men (32%) and 15 women (68%). The median tumor diameter was 5.3 cm (range, 1.0-10.0), and preoperative imaging suggested an initial diagnosis of GISTs in 7 patients (32%). Histologically, Verocay bodies were observed in 10 tumors (45%), vascular hyalinization in 13 (59%), discrete lymphoid aggregates in 5 (23%), and plasma cells in 8 (36%). Immunohistochemically, all tumors were positive for the gastric schwannoma markers S100 and SOX10, had variable CD34 expression, and were negative for α-smooth muscle actin and the GIST markers CD117 (KIT) and DOG1 (ANO1). Our findings aligned with previously reported demographic patterns and gastric schwannoma immunoprofiles; however, we also observed several histologic features less frequently described in the literature. Our observations refine the histopathologic characterization of gastric schwannomas and underscore the importance of thorough tissue evaluation for accurate diagnosis of this rare tumor. - Source: PubMed
Publication date: 2026/05/14
Diaz Antonio LiraDin Nasir UdKhan ManahilSayeed Bushra ZAhsan Beena U - Female fertility relies heavily on the health of the oviduct and uterus. Each of these organs consist of a muscular tube lined by epithelial cells. In this review, we discuss the role of the TMEM16A chloride ion channel, expressed in the epithelial and smooth muscle cells in these organs, with a focus on function and dysfunction. In the oviduct, infection and endometriosis are major causes of infertility and TMEM16A plays a major role. Oviduct epithelial TMEM16A is also essential for egg and sperm health and for the process of fertilization, which takes place in the oviduct. The role of TMEM16A in uterine function has focussed on the smooth muscle component. TMEM16A plays a major role in determining the sustained plateau component of the action potential in human myometrium. The plateau sits at the chloride equilibrium potential of -25 to -30 mV and, at this potential, calcium channels are active, permitting calcium influx and contraction. TMEM16A expression doubles in human labour and this is associated with a major increase in plateau and hence contraction duration. This prolongation of calcium influx permits contraction amplitude and duration to increase and appears to be important for successful labour. - Source: PubMed
Publication date: 2026/05/13
Parkington Helena CColeman Harold ASheehan Penelope MBrennecke Shaun PTare Marianne - Ca-activated Cl- conductances are present in many cell types and are important for regulating membrane potential as well as other cellular functions. TMEM16A is widely accepted as the principal molecular basis for Ca-activated Cl- conductances, but also members of the bestrophin family may be important for some Ca-activated Cl- conductances. - Source: PubMed
Aalkjær ChristianDam Vibeke SecherNilsson HolgerBoedtkjer Donna BMatchkov Vladimir