Ask about this productRelated genes to: TXNDC16 antibody
- Gene:
- TXNDC16 NIH gene
- Name:
- thioredoxin domain containing 16
- Previous symbol:
- KIAA1344
- Synonyms:
- -
- Chromosome:
- 14q22.1
- Locus Type:
- gene with protein product
- Date approved:
- 2003-11-21
- Date modifiied:
- 2018-11-22
Related products to: TXNDC16 antibody
Related articles to: TXNDC16 antibody
- Breast cancer is highly heterogeneous, with multiple subtypes that differ in molecular and clinical characteristics. It remains the most common cancer among women worldwide. We conducted a hypothesis-generating study using a bioinformatics approach in order to identify potential prognostic biomarkers for breast cancer patients across multiple molecular subtypes. Given the influential role of the transforming growth factor beta (TGFB) pathway in shaping the immune microenvironment, we focused on the isoform, transforming growth factor beta 2 (), which is upregulated in tumors, to identify -dependent and -independent biomarkers for breast cancer patients' overall survival (OS) responses. We evaluated the impact of mRNA expression, in conjunction with other potential prognostic markers, on overall survival (OS) in breast cancer patients using The Cancer Genome Atlas (TCGA) and KMplotter databases. We employed a multivariate Cox proportional hazards model to compute hazard ratios (HRs) for mRNA expression, integrating an interaction term that accounts for the multiplicative relationship between and marker gene expressions while controlling age at diagnosis and cancer subtype and differentiating between patients receiving chemotherapy alone and those undergoing alternative therapeutic interventions. We used the KMplotter database to confirm -independent prognostic markers from TCGA data. In cases dependent on , increased mRNA expression of alongside higher levels of , , , , , , or was correlated with improved OS among breast cancer patients, of which four genes were upregulated in tumor tissues (, , , ). Future studies will be required to confirm breast cancer patients could improve OS outcomes for patients expressing high levels of and the marker genes in prospective clinical trials. Additionally, multivariate analysis revealed that the elevated expression of six genes (, , , , , ) were correlated with increases in HR, independent of mRNA expression; all except were identified as druggable targets. Future investigations assessing protein expression in breast cancer tumors to confirm the results of our retrospective analysis of mRNA levels will determine whether the protein products of these genes represent viable therapeutic targets. Protein-protein interaction (STRING) analysis indicated that TGFB2 is associated with EGFR and MYC from the PAM50 breast cancer gene signature. These findings suggest that correlation of -related markers could potentially complement the PAM50 signature in the assessment of OS prognosis in breast cancer patients, but further validation of the TGFB2/EGFR/MYC proteins in tumors is warranted. - Source: PubMed
Publication date: 2025/11/29
Qazi SanjiveRichardson StephenPotts MikeMyers ScottTrieu Vuong - In a previous study, we identified thioredoxin domain containing 16 (TXNDC16) as a meningioma-associated Ag by protein macroarray screening. Serological screening detected autoantibodies against TXNDC16 exclusively in meningioma patients' sera and not in sera of healthy controls. TXNDC16 was previously found to be an endoplasmic reticulum (ER)-luminal glycoprotein. In this study, we show an additional ER-associated localization of TXNDC16 in the cytosol by in vitro synthesis, molecular mass shift assay, and flow cytometry. We were able to show TXNDC16 secretion in different human cell lines due to masked and therefore nonfunctional ER retrieval motif. A previously indicated exosomal TXNDC16 secretion could not be confirmed in HEK293 cells. The secreted serum protein TXNDC16 is bound in circulating immune complexes, which were found both in meningioma and healthy blood donor sera. Employing a customized array with 163 overlapping TXNDC16 peptides and measuring autoantibody reactivity, we achieved discrimination of meningioma sera from healthy controls with an accuracy of 87.2% using a set of only five immunogenic TXNDC16 epitopes. - Source: PubMed
Publication date: 2014/08/13
Harz ChristianLudwig NicoleLang SvenWerner Tamara VGalata ValentinaBackes ChristinaSchmitt KatjaNickels RuthKrause ElmarJung MartinRettig JensKeller AndreasMenger MichaelZimmermann RichardMeese Eckart - In the endoplasmic reticulum (ER), members of the protein disulfide isomerase (PDI) family perform critical functions during protein maturation. Herein, we identify the previously uncharacterized PDI-family member ERp90. In cultured human cells, we find ERp90 to be a soluble ER-luminal glycoprotein that comprises five potential thioredoxin (Trx)-like domains. Mature ERp90 contains 10 cysteine residues, of which at least some form intramolecular disulfides. While none of the Trx domains contain a canonical Cys-Xaa-Xaa-Cys active-site motif, other conserved cysteines could endow the protein with redox activity. Importantly, we show that ERp90 co-immunoprecipitates with ERFAD, a flavoprotein involved in ER-associated degradation (ERAD), through what is most likely a direct interaction. We propose that the function of ERp90 is related to substrate recruitment or delivery to the ERAD retrotranslocation machinery by ERFAD. - Source: PubMed
Publication date: 2011/02/16
Riemer JanHansen Henning GAppenzeller-Herzog ChristianJohansson LindaEllgaard Lars