Ask about this productRelated genes to: SLC4A2 antibody
- Gene:
- SLC4A2 NIH gene
- Name:
- solute carrier family 4 member 2
- Previous symbol:
- EPB3L1, AE2
- Synonyms:
- HKB3, BND3L, NBND3
- Chromosome:
- 7q36.1
- Locus Type:
- gene with protein product
- Date approved:
- 1986-01-01
- Date modifiied:
- 2016-02-17
Related products to: SLC4A2 antibody
Related articles to: SLC4A2 antibody
- Prostate cancer remains a major health concern, ranking as the second leading cause of cancer-related deaths in men in the United States. The dissemination of tumor cells from the prostate, their entry into circulation, and subsequent skeletal metastasis involve complex mechanisms that are not fully elucidated. Notably, disseminated tumor cells demonstrate a remarkable ability to home to the hematopoietic stem cell niche within bone marrow, where they may remain dormant for years. Key signaling pathways implicated in dormancy include TGF-β, BMP4/BMP7, GAS6/TAM receptors, and Wnt5a, each influencing cell cycle arrest, survival, and phenotype adaptation. Recent research has explored analogies between dormancy mechanisms in cancer and plant biology, particularly focusing on phytohormones such as abscisic acid and gibberellins, which regulate plant stress responses and developmental dormancy. While plants utilize PYR1/PYL/RCAR receptors for abscisic acid, mammals rely on LANCL2 and PPARγ. This study evaluated the effects of abscisic acid, gibberellic acid, and the ABA agonist pyrabactin on human and murine prostate cancer cell lines. Results demonstrated that gibberellic acid lacked proliferative effects and could not counteract ABA-induced growth arrest. In contrast, pyrabactin potently induced growth arrest and apoptosis, activating SMAC/Diablo cell death pathways independently of LANCL2 and PPARγ signaling. Further, the activity of abscisic acid and pyrabactin depended on cellular uptake via SLC4AE2 and SLC4A3 anion exchangers; downregulation of these transporters partially reversed their inhibitory effects. These findings suggest a mechanistic parallel between phytohormone-induced dormancy in plants and regulated dormancy and apoptosis in PCa, opening new avenues for therapeutic targeting of dormancy pathways in cancer metastasis. - Source: PubMed
Rodriguez AgustinaJung YounghunParajuli Keshab RajTaichman Russell S - Ca-permeable transient receptor potential channels, which play an important role in the developmental/reactionary dentinogenesis by odontoblasts, are sensitive to wasabi sulfinyls. We investigated the effects of the wasabi sulfinyls 6-methylsulfinylhexyl isothiocyanate (6-MSITC) and its eight derivatives on ion transport mechanisms which promote mineralisation by odontoblasts. 6-MSITC significantly increased the mineralisation efficiency in cultured odontoblasts, and we also observed a significant increase in medium pH. Inhibitors of carbonic anhydrase (CA) and plasma membrane Ca-ATPase (PMCA) significantly reduced 6-MSITC-induced mineralisation. Odontoblasts expressed the HCO -transporting solute carrier family 4 (SLC4A) members SLC4A1, SLC4A2, SLC4A3, SLC4A4, SLC4A8 and SLC4A9, as well as CA I and CA II. 6-MSITC enhanced reactionary dentinogenesis beneath the cavities prepared on rat mandibular first molars. We recorded 6-MSITC-induced outward currents, which were suppressed by inhibitors of CA, Na-H exchanger (NHE), and Na-Ca exchanger (NCX). These results indicated that 6-MSITC has a strong ability to form reactionary dentin by activating or upregulating intracellular CA and electrically neutral HCO /H transport via SLC4As/NHE. Exchanging Na with H using NHE resulted in the reversal of the transmembrane Na gradient. This activated the Ca influx mode of NCX, and the subsequent accumulation of intracellular Ca was then extruded by PMCA activity to produce reactionary dentin. Thus, 6-MSITC activates CA-mediated SLC4As-NHE-NCX-PMCA coupling and is useful in dentin regenerative medicine. KEY POINTS: Ca signalling in odontoblasts plays an important role not only in developmental/reactionary dentinogenesis, but also in the generation of dentinal (tooth) pain. The wasabi sulfinyls including 6-methylsulfinylhexyl isothiocyanate (6-MSITC) promote mineralisation by odontoblasts and significantly increase pH in medium with cultured odontoblasts. We showed that 6-MSITC has a strong ability to form reactionary dentin through the upregulation and activation of intracellular carbonic anhydrase (CA) and electrically neutral HCO /H transport by members of the HCO -transporting solute carrier family 4 (SLC4As) and Na-H exchanger (NHE). Na accumulation by NHE activity resulted in the reversal of the transmembrane Na gradient. This activated the Ca influx mode of the Na-Ca exchanger (NCX), resulting in the accumulation of intracellular Ca. It was then extruded using plasma membrane Ca-ATPase (PMCA) to produce reactionary dentin. 6-MSITC activates CA-mediated SLC4As-NHE-NCX-PMCA coupling and is useful in dentin regenerative medicine. - Source: PubMed
Publication date: 2026/02/24
Furusawa YoshiakiKimura MakiOkunishi IsaoOuchi TakehitoKurashima RyuyaKatou-Yamada TomoeKuroda HidetakaSugita MakotoFurusawa MasahiroShibukawa Yoshiyuki - Bisphenol S (BPS) is frequently utilized in the domain of food packaging materials as well as in the sphere of industrial plastic manufacturing. Although existing studies suggest potential associations between BPS exposure and the development of various malignancies, the exact biological function of BPS in colorectal cancer (CRC) advancement is still not fully understood. This research intends to thoroughly explore how BPS influences the malignant characteristics of CRC at the molecular level, particularly its impact on the epithelial-mesenchymal transition (EMT) in tumor cells. This study employed a LASSO regression model to screen key genes associated with BPS exposure in CRC progression and constructed a prognostic prediction model. The ESTIMATE algorithm was employed to evaluate the stromal and immune scores within the tumor microenvironment, while the CIBERSORT technique was applied to examine the infiltration profiles of 22 distinct immune cell subsets. The impact of BPS on HCT8 cell proliferation, migratory ability, and EMT progression were systematically evaluated through Western blot, CCK-8 proliferation assays, scratch wound healing experiments, and Transwell assays for migration and invasion. BPS exposure within the concentration range of 10⁻¹ ⁰ to 10⁻⁶ M did not significantly inhibit HCT8 cell viability but markedly enhanced migration and invasion capabilities, accompanied by EMT progression. A prognostic model constructed from nine key genes linked to BPS and determined via LASSO regression exhibited robust predictive efficacy. Tumor microenvironment analysis indicated a substantial association between SLC4A2 gene expression and activated memory CD4 + T lymphocyte infiltration. Notably, BPS exposure specifically induced SLC4A2 upregulation, while siRNA-mediated SLC4A2 knockdown significantly suppressed BPS-induced cell migrationand reversed EMT phenotypes. This study first demonstrates that BPS promotes CRC cell migration by activating the EMT signaling pathway via SLC4A2 upregulation. SLC4A2, being a key player in BPS-induced tumorigenesis, could function as a promising biomarker and therapeutic target for CRC. These findings not only elucidate the molecular mechanisms underlying environmental pollutant-induced carcinogenesis but also provide experimental evidence for developing prevention strategies targeting BPS-associated CRC risks. - Source: PubMed
Publication date: 2025/07/14
Yalikun AbudushalamuCai JinfengZhou RunkaiChen HaonanPan JiaweiYusufu PaherediniLi Jikun - Eggshells not only protect the contents of the egg from external damage but are also a key factor influencing consumer choice, second only to price. In the later stages of egg production, the incidence of pimpled eggs significantly increases, severely affecting the hatchability and food safety of the eggs. This study compares the differences in the uterine proteomes and metabolomes of hens producing pimpled eggs and those producing normal eggs, aiming to identify the proteins and metabolites that may play a crucial role in the formation of pimpled eggs. A total of 242 differentially expressed proteins (DEPs) were identified in uterine tissue, of which 116 were upregulated and 126 were downregulated. Enrichment analysis revealed that the DEPs were enriched in pathways related to ion transport, energy metabolism, and immune responses. The study found that in the normal eggs (NE) group, HCO₃⁻ was predominantly transported via SLC4A1, although other transport pathways may also play a role. In contrast, in the pimpled eggs (PE) group, bicarbonate ions (HCO₃⁻) was primarily transported through SLC4A4. Additionally, a total of 44 differentially metabolites (DMs) were identified in the uterus, with 5'-Adenylic acid (ATP) being significantly downregulated in the PE group. The ions and matrix proteins required for eggshell formation are transported from uterine cells to the uterine fluid against a concentration gradient, a process that consumes a substantial amount of energy. The decrease in ATP concentration in the PE group may be a significant factor influencing the formation of pimpled eggs. Subsequently, we found that the DEPs and DMs were jointly enriched in several signaling pathways, including the FoxO signaling pathway related to energy metabolism, nicotinate and nicotinamide metabolism, and tryptophan metabolism associated with immune response. Notably, the DMs involved in these signaling pathways were all downregulated in the PE group. Our research findings indicate that SLC4A1, SLC4A2, and ATP2B4 (DEPs), along with 5'-adenylic acid and trigonelline (DMs), influence the formation of eggshells through mechanisms related to energy metabolism, ion transport, and immune response. These DEPs and DMs may serve as potential biomarkers for the genetic improvement of eggshell quality. - Source: PubMed
Publication date: 2025/01/15
Li WenqiangMa XueyingLi XiaominZhang XuguangSun YifeiNing ChaoZhang QinWang DanTang Hui - pH homeostasis is crucial for spermatogenesis, sperm maturation, sperm physiological function, and fertilization in mammals. HCO and H are the most significant factors involved in regulating pH homeostasis in the male reproductive system. Multiple pH-regulating transporters and ion channels localize in the testis, epididymis, and spermatozoa, such as HCO transporters (solute carrier family 4 and solute carrier family 26 transporters), carbonic anhydrases, and H-transport channels and enzymes (e.g., Na-H exchangers, monocarboxylate transporters, H-ATPases, and voltage-gated proton channels). Hormone-mediated signals impose an influence on the production of some HCO or H transporters, such as NBCe1, SLC4A2, MCT4, etc. Additionally, ion channels including sperm-specific cationic channels for Ca (CatSper) and K (SLO3) are directly or indirectly regulated by pH, exerting specific actions on spermatozoa. The slightly alkaline testicular pH is conducive to spermatogenesis, whereas the epididymis's low HCO concentration and acidic lumen are favorable for sperm maturation and storage. Spermatozoa pH increases substantially after being fused with seminal fluid to enhance motility. In the female reproductive tract, sperm are subjected to increasing concentrations of HCO in the uterine and fallopian tube, causing a rise in the intracellular pH (pH) of spermatozoa, leading to hyperpolarization of sperm plasma membranes, capacitation, hyperactivation, acrosome reaction, and ultimately fertilization. The physiological regulation initiated by SLC26A3, SLC26A8, NHA1, sNHE, and CFTR localized in sperm is proven for certain to be involved in male fertility. This review intends to present the key factors and characteristics of pH regulation in the testes, efferent duct, epididymis, seminal fluid, and female reproductive tract, as well as the associated mechanisms during the sperm journey to fertilization, proposing insights into outstanding subjects and future research trends. - Source: PubMed
Publication date: 2024/06/12
Dai PengyuanZou MengCai ZiyiZeng XuhuiZhang XiaoningLiang Min