Ask about this productRelated genes to: SLC5A5 antibody
- Gene:
- SLC5A5 NIH gene
- Name:
- solute carrier family 5 member 5
- Previous symbol:
- -
- Synonyms:
- NIS
- Chromosome:
- 19p13.11
- Locus Type:
- gene with protein product
- Date approved:
- 1997-04-16
- Date modifiied:
- 2016-02-17
Related products to: SLC5A5 antibody
Related articles to: SLC5A5 antibody
- Thyroid hormone (TH) synthesis and secretion can be perturbed by endocrine disrupting chemicals (EDCs). Traditional 2D cell culture models are useful for studying specific effects on enzymes and transporters involved in hormone synthesis; however, they lack the structural organization required for hormone production. In this study, we refine the conditions for an rat thyroid gland model that supports robust thyroxine (T4) production and to evaluate the inhibition of T4 output using a model inhibitor. Thyroid glands from postnatal day 6 were excised and cultured for 7 or 9 days. We assessed the influence of medium composition (RPMI or StemPro™-34), culture method (hanging drop or filter inserts), thyroid stimulating hormone (TSH) concentration and iodide supplementation by measuring T4 secretion into the culture medium, evaluating tissue morphology and quantifying expression of key genes in TH synthesis machinery. Cultivating on filter inserts in StemPro™-34 media with iodide addition and 5 mU/mL TSH was the superior condition for enhanced T4 production while preserving tissue morphology. By challenging the model with the antithyroid drug methimazole (MMI) T4 release was markedly reduced after 9 days of culture, and gene expression levels of and (NIS) were significantly upregulated. Together, these results demonstrate that the optimized vivo rat model supports sustained T4 production and is sensitive to a TH synthesis inhibitor, making it a promising model for studying the thyroid gland and the effects of thyroid-disrupting chemicals. - Source: PubMed
Publication date: 2026/03/07
Melchiors MikalaStub MetteRamhøj LouiseBarmpari EleniTran Kieu-MiBindel Anna OpstrupRosenmai Anna KjerstineSvingen Terje - Pyrethroid pesticides are recognized endocrine disruptors that can disrupt hormonal homeostasis in vertebrates, particularly thyroid function. This study investigated whether an ethanolic extract of Paulownia tomentosa leaves (PLE) could alleviate lambda-cyhalothrin-induced (LCT) thyroid toxicity in mice. The phenolic profile of PLE was confirmed by HPLC, and cytotoxicity was assessed using the MTT assay, which identified 9 mg/kg body weight as a suitable dose. Twenty adult male mice were divided into four groups: control, PLE alone (9 mg/kg), LCT alone (2 mg/kg), and combined PLE+LCT treatment for 28 days. LCT exposure produced marked thyroid dysfunction, reflected by increased serum TSH and reduced T₃ and T₄, accompanied by elevated MDA and pro-inflammatory cytokines, reduced antioxidant enzyme activities, and intensified apoptotic responses. Gene expression analysis demonstrated significant downregulation of thyroid hormone synthesis genes (Slc5a5/NIS, Tpo, Tg) and antioxidant defense markers (Nfe2l2/Nrf2, Hmox1/HO-1), alongside pronounced upregulation of inflammatory genes (Tnf, Il1b) and pro-apoptotic mediators (Bax, Casp3), with suppression of Bcl2. Histological alterations included follicular degeneration, colloid depletion, and reduced follicle diameter. Co-administration of PLE substantially attenuated these biochemical, molecular, and structural alterations, restoring thyroid-related and antioxidant gene expression, reducing inflammatory and apoptotic markers, and partially normalizing follicular morphology. These protective effects likely relate to the phenolic constituents of PLE, which possess recognized antioxidant and anti-inflammatory activities. Overall, PLE demonstrates potential as a natural complementary agent against LCT-induced thyroid injury. Further studies are warranted to isolate the bioactive compounds and clarify their mechanisms. - Source: PubMed
Publication date: 2026/02/26
El-Hashash Samah AElkholy Ethar EEl-Gawish Asmaa MShukry MustafaAlbokhadaim IbrahimAlhojaily SameerAl Khodair Khalid M - The sodium-iodide symporter (NIS, SLC5A5) plays a crucial role in thyroid hormone synthesis. Especially during brain development, correct thyroid signaling is of critical importance. Hence, inhibition of this transporter can lead to neurodevelopmental disorders, such as lowered IQ or autism. In order to uncover environmental chemicals with the potential of causing developmental neurotoxicity (DNT), NIS was selected for modeling. To support next-generation risk assessment, in silico-based methods were utilized. Docking-based virtual screening workflows of a library of compounds with experimentally determined inhibitory activity on NIS were applied. In addition, machine learning (ML) models based on random forest (RF), extreme gradient boosting (XGB), and support vector machines (SVM) were trained using extended-connectivity fingerprints 4 (ECFP4) and continuous and data-driven descriptors (CDDDs) with 9-fold cross validation to discriminate between NIS inhibiting and noninhibiting compounds. Ultimately, combining ML and docking predictions improved discrimination, achieving an area under the receiver operating characteristic curve (ROC AUC) of 0.77. Thresholds for optimal discrimination between actives and inactives were determined using kernel density estimate plots, at which a Matthews correlation coefficient (MCC) of 0.32, and a balanced accuracy (BA) of 0.78 were achieved on the internal test set. By combining ML predictions with docking scores and training on a larger, more diverse data set of 1412 compounds, this study provides a novel and robust framework for NIS inhibition prediction, which constitutes a new approach method in toxicological risk assessment. - Source: PubMed
Publication date: 2026/01/23
Kandler JuliaKantarçeken Ayse SılaSmajić AljošaEcker Gerhard F - Thyroid cancer is the most common endocrine malignancy globally. While papillary thyroid carcinoma (PTC) typically exhibits favorable prognosis, a subset undergoes dedifferentiation into anaplastic thyroid carcinoma (ATC), an aggressive, treatment-refractory subtype with near-universal lethality. However, the molecular driver of this process remains elusive. In this study, we find that IGF2BP2 is upregulated in ATC and correlates with adverse prognosis. Pseudotime trajectory analysis tracks progressively escalating IGF2BP2 expression throughout dedifferentiation. Functionally, IGF2BP2 promotes proliferation, suppresses thyroid differentiation genes (, , , , , , and ), and enhances cancer stemness. Mechanistically, integrated multi-omics analysis (RNA-seq, RIP-seq, and MeRIP-seq) reveals that IGF2BP2 binds m6A-modified mRNA, accelerating its decay. STAT1 directly activates transcription of thyroid differentiation genes. Rescue experiments confirms that STAT1 mediates IGF2BP2-driven dedifferentiation. The IGF2BP2-m6A-STAT1 complex is a master regulator of thyroid cancer dedifferentiation, establishing a novel therapeutic target for redifferentiation therapy in advanced thyroid cancer. - Source: PubMed
Publication date: 2026/01/01
Chen RuiLi Yi-XunLu Wei-LinWu Ke-FeiWang Yu-XinWang Zi-WenLi Yi-HanYang Hai-YanZhang XuShi LiangZhou DongWang YingDing Qiang - : Iodine plays a key role in thyroid hormone synthesis and metabolic regulation in vertebrates. This study aimed to evaluate the in vivo bioavailability of iodine and assess selected biochemical parameters and thyroid-related gene expression in male Wistar rats fed lettuce ( L.) biofortified with iodoquinolines (8-hydroxy-7-iodo-5-quinolinesulfonic acid or 5,7-diiodo-8-quinolinol) or potassium iodate. : Two iodine intake levels were applied, a nutritionally adequate iodine level and a supranutritional level, to evaluate the nutritional safety of iodine obtained from biofortified vegetables. : A diet containing lettuce biofortified with iodoquinolines at the adequate level had no significant effect on thyroid hormone concentrations, the expression of , , , and genes, or thyroid morphology. While supranutritional iodine intake led to increased levels of T4, fT4, T3, and fT3, all hormone concentrations remained within the physiological range. No elevation in liver enzyme activity (ALT, AST, ALP) was observed, indicating the absence of hepatotoxic effects from high-iodine diets based on biofortified lettuce. Compared to potassium iodate, iodoquinolines demonstrated superior bioavailability, as evidenced by enhanced iodine accumulation in tissues and more efficient thyroid hormone synthesis. : To the best of our knowledge, this is the first in vivo nutritional study assessing the physiological effects of supranutritional iodine intake from a biofortified plant source. These findings confirm the nutritional safety and efficacy of iodine biofortification using iodoquinolines and highlight the need for further research, including human nutritional clinical trials. - Source: PubMed
Publication date: 2025/12/21
Dyląg AgnieszkaPawlicki PiotrGałuszka AnnaSmoleń SylwesterKoronowicz Aneta