Ask about this productRelated genes to: FMO3 antibody
- Gene:
- FMO3 NIH gene
- Name:
- flavin containing monooxygenase 3
- Previous symbol:
- -
- Synonyms:
- -
- Chromosome:
- 1q24.3
- Locus Type:
- gene with protein product
- Date approved:
- 1992-10-16
- Date modifiied:
- 2018-05-03
Related products to: FMO3 antibody
Related articles to: FMO3 antibody
- Understanding oxidative metabolism of organophosphate pesticides (OPs) in humans is critical for toxicological risk assessment. Electrode-driven bioelectrocatalysis enables enzyme catalysis without added electron donors (e.g. NADPH), providing simplified in vitro models of xenobiotic metabolism. Here we report a tandem configuration of two sequential bioelectrodes that reproduces fenthion oxidation using immobilized human flavin-containing monooxygenase 3 (hFMO3) and cytochrome P450 2C19 (CYP2C19), two key hepatic enzymes involved in OPs detoxification. Glassy carbon electrodes were coated with dimethyldidodecylammonium bromide (DDAB) or DDAB-stabilized gold nanoparticles (AuNPs-DDAB) to form stable enzyme films and promote direct electron transfer. AuNPs-DDAB dispersions displayed bimodal hydrodynamic diameter distribution (∼20 and 160 nm) and a positive ζ-potential across pH 2-9. Cyclic voltammetry showed reversible redox couples for both enzymes, higher currents on AuNPs-DDAB, and a positive midpoint shift for CYP2C19. hFMO3 bioelectrodes catalysed fenthion S‑oxygenation, and electrochemical titration gave K = 32.8 ± 6.3 μM, comparable to solution assays. Tandem chronoamperometry enabled sequential conversion of fenthion to sulfoxide by hFMO3 and further oxidation by CYP2C19 to sulfone and oxon sulfone. Overall, this sequential bioelectrodes system provides an electrochemically tunable route to map pesticide oxidation pathways and profile human-relevant metabolites without added NADPH or cytochrome P450 reductase. - Source: PubMed
Publication date: 2026/04/08
Dong ShimanDe Angelis MelissaCatucci GianlucaCesano FedericoMarucco AriannaGilardi GianfrancoSadeghi Sheila J - Non-alcoholic fatty liver disease (NAFLD) is the leading chronic liver disease globally, characterized by steatosis, inflammation, and hepatocyte injury. While the Flavin-containing monooxygenase 3 (FMO3)-trimethylamine N-oxide (TMAO) axis is a known driver of atherosclerosis, its role in NAFLD progression remains unclear. Here, we report that FMO3 is significantly upregulated in the livers of choline-deficient, high-fat diet (CDA-HFD)-induced NAFLD mice, as revealed by transcriptomic profiling and validation. Mechanistically, FMO3 overexpression exacerbated lipid accumulation and inflammatory cytokine release in vitro, while its metabolite TMAO directly aggravated hepatic steatosis and inflammation in vivo. Notably, the natural compound 3,3'-diindolylmethane (DIM) significantly attenuated NAFLD phenotypes, including serum ALT/AST levels and hepatic lipid content; however, these protective effects were reversed by exogenous TMAO supplementation. These findings identify the FMO3-TMAO axis as a critical target for regulating lipid homeostasis and inflammation, suggesting DIM as a promising therapeutic candidate for NAFLD intervention. - Source: PubMed
Publication date: 2026/03/18
Chen RunshengLiu HuaYang TinfengJiang ZhonghaoHe ZeyuZhang XinpengQian BaolinFu Wenguang - Indigo is an important blue pigment widely used in textile, food, and pharmaceutical industries. Currently, most of indigo was produced via chemical synthesis, which led to pollution to the environment and potential health hazards to consumers. Thus, biological synthesis of indigo attracts increasing attention. Flavin-containing monooxygenase (FMO) is an important enzyme for production of indigo from indole, however, only five indigo-producing FMOs have been identified. In this study, a novel indigo-producing FMO from Pseudomonas guineae was successfully screened from seven candidates. The recombinant enzyme was comprehensively studied regarding its expression, purification, and identification. The purified enzyme had two absorbance peaks at 360 and 442 nm, indicating the binding of cofactors. It showed the highest activity at pH 8.0 and 20 °C. The K, V, k, and k/K were 2.5 mM, 2.9 × 10 mM/min, 0.027 s, and 0.011 mM s, respectively. The in vivo indigo production of P. guineae FMO was evaluated by metabolically engineered E. coli BL21(DE3). After deleting the competitive genes, strengthening the tryptophan biosynthetic pathway, and optimizing indole transportation, indigo was effectively produced with a titer of 260 mg/L by shake-flask cultivation. - Source: PubMed
Publication date: 2026/02/20
Hao ZhebinZhang YuleiZhang WenliZhu YingyingMu Wanmeng - Flavin-containing monooxygenase 3 (FMO3) is a key metabolic enzyme involved in endogenous metabolism; however, its role in colorectal cancer (CRC) remains poorly understood. This study aimed to investigate the expression pattern, biological function, and potential clinical relevance of FMO3 in CRC. - Source: PubMed
Publication date: 2026/02/21
Su RongHuang CuncunJing ZheQiu ShupingShao LihuaJiao TinghongWang HongweiLi Hailong - Voriconazole (VRC), a triazole antifungal agent, is recommended as primary treatment for invasive aspergillosis. Due to its unpredictable dose-exposure relationship, therapeutic drug monitoring (TDM) is recommended. Despite TDM, the probability of achieving therapeutic targets is only approximately 50%. This highlights the need for new dosing strategies. Calculating the metabolic ratio (MR) of NOX, the principal N-oxide metabolite of voriconazole primarily formed by the genetically polymorphic CYP2C19 enzyme, to VRC concentrations could serve as a surrogate for phenotype information. This is particularly relevant since the CYP2C19 genotype is often unavailable in acute clinical settings. Moreover, genotype may be less informative during inflammation due to a phenomenon known as 'phenoconversion', where inflammation masks genotype effects through downregulation of CYP450 enzymes. However, before integrating NOX in population PK models and/or as an adjunct in voriconazole TDM flowcharts, it is important to understand the factors impacting NOX exposure, and hence the MR. The objective of this narrative review is therefore to summarise recent literature on patient- and drug-related covariates impacting NOX exposure. - Source: PubMed
Hubrechts JanneGijsen MatthiasFloré KatelijneGrootaert VeerleLemaitre FlorianBoglione-Kerrien ChristelleMertens BeatrijsAnnaert PieterLagrou KatrienSpriet Isabel