Ask about this productRelated genes to: MUC3B antibody
- Gene:
- MUC3B NIH gene
- Name:
- mucin 3B, cell surface associated
- Previous symbol:
- -
- Synonyms:
- -
- Chromosome:
- 7q22 not on reference assembly
- Locus Type:
- gene with protein product
- Date approved:
- 2000-09-27
- Date modifiied:
- 2015-02-09
Related products to: MUC3B antibody
Related articles to: MUC3B antibody
- High-temperature (HT) is a critical influencing factor in shrimp aquaculture and serves as a key trigger for frequent disease outbreaks in shrimp. As a core organ for digestion, absorption and immune defense, the intestine's functional homeostasis is the key foundation for shrimp health. Therefore, in this study, the shrimp Litopenaeus vannamei were continuously exposed to HT stress at 33 °C for 7 days, after which the changes in intestinal functional homeostasis were investigated based on the mucosal integrity, immune signaling, and microbial community. The results showed that HT stress caused intestinal epithelial detachment and mucosal damage, as well as the disruption of the mucus barrier, including the upregulation of MUC2, MUC3A and MUC3B genes and the downregulation of MUC1 and MUC4 genes. Additionally, oxidative stress-related genes, such as the expressions of ROMO1, Nrf2, GPx and HO1 were upregulated, while the expression of SOD was downregulated; antimicrobial genes, such as the expressions of Crus and proPO were upregulated, whereas the expressions of ALF, Pen3 and Lys were downregulated; inflammatory genes (JNK and NF-κB) and autophagy genes (Atg3, Atg12, Beclin1 and Hsc70) expression were all upregulated. In terms of intestinal microbiota, microbial diversity showed no significant changes, but the abundance of community composition was perturbed, particularly the homeostasis of putative harmful bacteria (Vibrio and Photobacterium) and beneficial bacteria (Bacteroides, Bacillus, Lactobacillus, and Lactococcus). Additionally, the phosphotransferase system (PTS) function of the intestinal microbiota was enhanced, while the functions such as N-glycan biosynthesis and glycosaminoglycan degradation were weakened. These results demonstrated that HT stress disrupted intestinal functional homeostasis by inducing mucosal damage, disrupting the mucus barrier and immune responses, activating oxidative stress, inflammation, and autophagy signaling, and reshaping the microbial community. - Source: PubMed
Publication date: 2025/09/02
Duan YafeiHuang JianhuaWang YunYang YukaiLi Hua - Aberrant mucus secretion is a hallmark of chronic obstructive pulmonary disease (COPD). Expression of the membrane-tethered mucins 3A and 3B (MUC3A, MUC3B) in human lung is largely unknown. In this observational cross-sectional study, we recruited subjects 45-65 years old from the general population of Stockholm, Sweden, during the years 2007-2011. Bronchial mucosal biopsies, bronchial brushings, and bronchoalveolar lavage fluid (BALF) were retrieved from COPD patients (n = 38), healthy never-smokers (n = 40), and smokers with normal lung function (n = 40). Protein expression of MUC3A and MUC3B in bronchial mucosal biopsies was assessed by immunohistochemical staining. In a subgroup of subjects (n = 28), MUC3A and MUC3B mRNAs were quantified in bronchial brushings using microarray. Non-parametric tests were used to perform correlation and group comparison analyses. A value of < 0.05 was considered statistically significant. MUC3A and MUC3B immunohistochemical expression was localized to ciliated cells. MUC3B was also expressed in basal cells. MUC3A and MUC3B immunohistochemical expression was equal in all study groups but subjects with emphysema had higher MUC3A expression, compared to those without emphysema. Smokers had higher mRNA levels of MUC3A and MUC3B than non-smokers. MUC3A and MUC3B mRNA were higher in male subjects and correlated negatively with expiratory air flows. MUC3B mRNA correlated positively with total cell concentration and macrophage percentage, and negatively with CD4/CD8 T cell ratio in BALF. We concluded that MUC3A and MUC3B in large airways may be a marker of disease or may play a role in the pathophysiology of airway obstruction. - Source: PubMed
Publication date: 2023/08/31
Merikallio HetaPincikova TereziaKotortsi IoannaKarimi RezaLi Chuan-XingForsslund HelenaMikko MikaelNyrén SvenLappi-Blanco ElisaWheelock Åsa MKaarteenaho RiittaSköld Magnus C - Evidence has shown that gut microbiota play a key role in host metabolism and health; however, little is known about the microbial community in the donkey hindgut as well as the interactions that occur between gut microbes and the host. This study aimed to explore the gut microbiome differences by analyzing the microbial community and differentially expressed genes (DEGs) related to lipid metabolism and the immune system along the donkey hindgut. The hindgut tissues (cecum, ventral colon, and dorsal colon) were separated, and the contents of each section were collected from six male donkeys for multi-omics analysis. There were significant differences in terms of dominant bacteria among the three sections, especially between the cecum and dorsal colon sites. For instance, species belonging to and were most abundant in the cecum, while the , , , etc., were more abundant in the dorsal colon. Apart from propionate, the concentrations of acetate, isobutyrate, valerate and isovalerate were all lower in the cecum than in the dorsal colon (p < 0.05). Furthermore, we identified some interesting DEGs related to lipid metabolism (e.g., , , , and ) and the immune system (e.g., , mucin-2-like, , , , , and ) between the cecum and dorsal colon and found that the PPAR pathway was mainly enriched in the cecum. Finally, we found a complex relationship between the gut microbiome and gene expression, especially with respect to the immune system, and combined with protein-protein interaction (PPI) data, suggesting that the PPAR pathway might be responsible, at least in part, for the role of the hindgut microbiota in the donkeys' gut homeostasis. Our data provide an in-depth understanding of the interaction between the microbiota and function in the healthy equine hindgut and may also provide guidance for improving animal performance metrics (such as product quality) and equine welfare. - Source: PubMed
Publication date: 2022/11/18
Li YanMa QingshanShi XiaoyuanLiu GuiqinWang Changfa - Human tissue surfaces are coated with mucins, a family of macromolecular sugar-laden proteins serving diverse functions from lubrication to the formation of selective biochemical barriers against harmful microorganisms and molecules. Membrane mucins are a distinct group of mucins that are attached to epithelial cell surfaces where they create a dense glycocalyx facing the extracellular environment. All mucin proteins carry long stretches of tandemly repeated sequences that undergo extensive O-linked glycosylation to form linear mucin domains. However, the repetitive nature of mucin domains makes them prone to recombination and renders their genetic sequences particularly difficult to read with standard sequencing technologies. As a result, human mucin genes suffer from significant sequence gaps that have hampered the investigation of gene function in health and disease. Here we leveraged a recent human genome assembly to characterize a previously unmapped MUC3B gene located at the q22 locus on chromosome 7, within a cluster of four structurally related membrane mucin genes that we name the MUC3 cluster. We found that MUC3B shares high sequence identity with the known MUC3A gene and that the two genes are governed by evolutionarily conserved regulatory elements. Furthermore, we show that MUC3A, MUC3B, MUC12, and MUC17 in the human MUC3 cluster are expressed in intestinal epithelial cells (IECs). Our results complete existing genetic gaps in the MUC3 cluster which is a conserved genetic unit in vertebrates. We anticipate our results to be the starting point for the detection of disease-associated polymorphisms in the human MUC3 cluster. Moreover, our study provides the basis for the exploration of intestinal mucin gene function in widely used experimental models such as human intestinal organoids and genetic mouse models. - Source: PubMed
Publication date: 2022/10/18
Lang TiangePelaseyed Thaher - Intestinal mucosal healing is nowadays preferred as the therapeutic endpoint in inflammatory bowel disease (IBD), but objective measurements at the molecular level are lacking. Because dysregulated mucin expression is suggested to be involved in mucosal barrier dysfunction in IBD, we investigated mucin expression in association with barrier mediators and clinical characteristics in colonic tissue of a pediatric IBD population. - Source: PubMed
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