Ask about this productRelated genes to: RNF19A antibody
- Gene:
- RNF19A NIH gene
- Name:
- ring finger protein 19A, RBR E3 ubiquitin protein ligase
- Previous symbol:
- RNF19
- Synonyms:
- dorfin, DKFZp566B1346
- Chromosome:
- 8q22.2
- Locus Type:
- gene with protein product
- Date approved:
- 2003-02-28
- Date modifiied:
- 2016-11-01
Related products to: RNF19A antibody
Related articles to: RNF19A antibody
- RNF19A is a poorly characterized ring between ring finger (RBR) E3 ubiquitin ligase that acts as a tumor suppressor in bladder cancer, whereas as an oncogene in non-small cell lung cancer. However, the biological functions of RNF19A in gastric cancer cells and whether RNF19A targets other substrates involved in gastric cancer progression remain unclear. Here, we report that RNF19A expression is elevated in gastric cancer tissues and is indicative of poor prognoses. - Source: PubMed
Publication date: 2026/01/07
Cheng YuLi ShuaiLi XiangXue JingHao MeilingZhou LiliLuo YuZhang YiJunLi Yuhong - Although immune checkpoint blockade (ICB) is an effective therapeutic strategy, tumor intrinsic resistance to ICB limits its benefits for a majority of cancer patients. Via multiple in vivo screens, we identified RNF19A as an immune suppressor. RNF19A suppresses ICB efficacy by ubiquitinating cGAS to inhibit the release of type I interferon. Surprisingly, UBE2L6, originally identified as a critical interferon-induced ISG15-conjugating E2 enzyme for ISGylation, functions as the major ubiquitin-conjugating E2 enzyme to enable RNF19A-driven cGAS ubiquitination. Further, RSK1 phosphorylates UBE2L6 to switch its substrate from ISG15 to ubiquitin, thus converting it from an immune response enhancer to a suppressor. In xenograft models, targeting RSK1 or blocking UBE2L6 phosphorylation improves immune response. In patient tissues, the levels of UBE2L6 and its phosphorylation correlate with poor immune infiltration, limited immune and radiation therapy response, and prognosis. Taken together, we demonstrate that RSK1-mediated UBE2L6 phosphorylation and UBE2L6-RNF19A-driven cGAS ubiquitination lead to tumor-intrinsic immune suppression. - Source: PubMed
Publication date: 2025/09/22
Peng QiaoChen YunfeiZhao JunmiaoZhu JialiZhuo HuiminZheng YuyingWeng LinjunRuiqing He Tian EnmingZhang YiyiChen XianfeiLiu YuweiTan XiaoWu LeileiTian HonglingLiu JingxinZhang JiawenZhang WenhuiZhang HepingZhang BilongShan ZezhiZhang HaoyuGao YaohuiMeng TongZhao LinlinFang LinShen BingFeng YueLiu LeiYang HuiFang LanWang Ping - Ubiquitin is a small, highly conserved protein that acts as a posttranslational modification in eukaryotes. Ubiquitination of proteins frequently serves as a degradation signal, marking them for disposal by the proteasome. Here we report a novel small molecule from a diversity-oriented synthesis library, BRD1732, that is directly ubiquitinated in cells, resulting in dramatic accumulation of inactive ubiquitin monomers and polyubiquitin chains, which causes broad inhibition of the ubiquitin-proteasome system. Ubiquitination of BRD1732 and its associated cytotoxicity are stereospecific and dependent on two homologous E3 ubiquitin ligases, RNF19A and RNF19B, and their shared E2 conjugating enzyme, UBE2L3. Our finding opens the possibility for indirect ubiquitination of a target through a ubiquitinated bifunctional small molecule and more broadly raises the potential for posttranslational modification in trans. - Source: PubMed
Publication date: 2025/08/21
Li WeichengGarcia-Rivera Enrique MMitchell Dylan CChick Joel MMaetani MicahBhadoria RohitKnapp John MMisu RyosukeMatthews Geoffrey MShirasaki Ryosukede Matos Simoes RicardoViswanathan VasanthiPulice John LRees Matthew GRoth Jennifer AGygi Steven PMitsiades Constantine SKadoch CigallSchreiber Stuart LOstrem Jonathan M L - Rheumatoid arthritis (RA) is a prevalent autoimmune disease, affecting approximately 1% of the global population. Methotrexate (MTX) is the most widely prescribed drug for RA treatment; however, its efficacy is often limited, with resistance frequently observed. Fibroblast-like synoviocytes (FLS) play a pivotal role in RA progression and are closely linked to drug resistance, although the underlying mechanisms remain poorly understood. In this study, we conducted a comprehensive analysis of public single-cell transcriptomics data from osteoarthritis and rheumatoid arthritis synovial tissues, identifying RNF19A as a gene potentially associated with RA resistance in FLS. Our findings indicate that RNF19A is significantly overexpressed in drug-resistant FLS and is closely associated with the dysregulation of FLS proliferation, migration, invasion, and apoptosis. Furthermore, we demonstrated that RNF19A promotes functional disruption in FLS by ubiquitinating and degrading MKP-1, thereby activating the MAPK signaling pathway. This activation also facilitates the nuclear translocation of ZBTB20, an upstream transcription factor of RNF19A, which further enhances RNF19A transcription. This biological process creates a positive feedback loop in FLS, contributing to RA resistance-a mechanism that was also validated in vivo. In summary, this study is the first to underscore the crucial role of RNF19A in mediating drug resistance in RA FLS, elucidating the underlying biological processes, and providing novel insights into RA pathogenesis, thereby offering a new experimental foundation for RA drug development. - Source: PubMed
Publication date: 2025/04/28
Luo XinTang NingRen YijunLi JingchenZhu HuanchengWu SongDing Zhiyu - Prostate cancer (PCa) is the most prevalent type of cancer and the second leading cause of mortality in males, with a marked increase in incidence observed across the globe. In the present study, whole-transcriptome analysis was conducted to identify differentially expressed circular RNAs (DE-circRNAs). The coding abilities of the DE-circRNAs were analyses, and it was found that hsa_circ_0085121 (circRNF19A) not only exhibited overexpression in PCa cells and tumor samples, but also encoded a 490 amino acid polypeptide designated circRNF19A-490aa. The knockdown of circRNF19A was observed to notably inhibit the proliferation, invasion, migration and docetaxel resistance of PCa cells. In contrast, mutation of the IRES significantly impaired the tumor-promoting function of circRNF19A, indicating that circRNF19A-490aa is the primary form that regulates the malignant behaviors of PCa cells. Mechanistically, circRNF19A-490aa was demonstrated to interact with HSP90AA1, thereby enhancing AR activity and facilitating the activation of the Akt/mTOR and PLK1 pathways. Furthermore, circRNF19A-490aa was observed to interact with HNRNPF, facilitating the recruitment of HNRNPF to the splicing site of AR-V7 and enhancing its alternative splicing. Finally, the androgen receptor (AR) was observed to bind to the promoter region of the RNF19A gene, subsequently regulating the expression of circRNF19A and circRNF19A-490aa. These data indicate that circRNF19A plays a pivotal role in AR activation and AR-V7 generation by encoding a novel protein, circRNF19A-490aa, and targeting circRNF19A may prove an effective strategy for impeding the progression of CRPC. - Source: PubMed
Publication date: 2024/11/20
Li JianfengQiu HuiDong QingzhuoYu HongyuanPiao ChiyuanLi ZhengxiuSun YanbinCui Xiaolu