Ask about this productRelated genes to: RNF19A antibody
- Gene:
- RNF19A NIH gene
- Name:
- ring finger protein 19A, RBR E3 ubiquitin protein ligase
- Previous symbol:
- RNF19
- Synonyms:
- dorfin, DKFZp566B1346
- Chromosome:
- 8q22.2
- Locus Type:
- gene with protein product
- Date approved:
- 2003-02-28
- Date modifiied:
- 2016-11-01
Related products to: RNF19A antibody
Related articles to: RNF19A antibody
- The hepatitis E virus (HEV) is a leading cause of acute hepatitis worldwide. HEV infection can become chronic in immunocompromised individuals, in whom virus-host recombinant variants (VHRVs) can be detected. These variants often harbor host-derived insertions in the polyproline-rich region (PPR), and most display enhanced replication . However, the mechanisms underlying this replicative advantage remain unclear. It is likely that genes of the infected cells are differentially expressed according to the replicative capacity of the strain. The host factors involved in the improvement of the replicative capacity of these VHRVs are yet to be identified.In this study, we analyzed the host transcriptional response to seven VHRVs in HepG2/C3A cells using bulk RNA sequencing at 48 h and 168 h post-infection. Five VHRVs (RNF19A, ZNF787, KIF1B, RPS17, EEF1A1) previously associated with a high replication rate induced more significant, distinct transcriptomic changes than low-replicative variants (RNA18, RPL6), particularly at 168 h. A shared set of 25 genes, especially interferon-stimulated genes (ISGs), was upregulated in cells infected with high-replicating variants. Interestingly, ISG induction was limited at 48 h despite high viral RNA concentrations, suggesting a delayed antiviral response. At 168 h, high ISG expression coincided with high viral loads, indicating that VHRVs may evade or exploit immune defenses. Our findings reveal candidate ISGs such as IFIT1 and ISG15 that may influence HEV persistence and immune escape. These results offer new insights into the interplay between VHRV replication and host immunity.IMPORTANCEHepatitis E virus (HEV) is a major cause of acute hepatitis and can cause chronic infections in immunocompromised individuals. Virus-host recombinant variants (VHRVs) having integrated host-derived insertions often replicate more effectively, yet the host determinants of this phenotype remain unclear. With RNA sequencing of HepG2/C3A-infected cells, we observed that high-replicating VHRVs induce a delayed but strong expression of interferon-stimulated genes (ISGs), including IFIT1 and ISG15, despite high viral loads. These results suggest that VHRVs may transiently modulate or evade aspects of host antiviral defenses. Our study revealed host transcriptional patterns associated with enhanced viral replication, providing insight into potential mechanisms that enhance HEV replication and highlighting candidate pathways that could influence the interplay between viral replication and immune responses, all requiring further investigation. - Source: PubMed
Publication date: 2026/07/13
Paronetto OliviaAllioux ClaireZahm MargotGonzalez Anne AliciaJeanne NicolasChaubet AdelineCollercandy NivedAbravanel FlorenceLhuillier ÉmelineChapuy-Regaud SabineIzopet JacquesLhomme Sébastien - In the uterus, PLAG1-rearranged mesenchymal tumors are increasingly recognized, most being myxoid leiomyosarcomas. Here we report a unique PLAG1-rearranged uterine mesenchymal tumor with myofibroblastic features and focal adipocytic differentiation in a 50‑year‑old woman. The 2.7‑cm tumor, preoperatively suspected as leiomyoma, showed bland spindle to epithelioid cells arranged in solid sheets or fascicles within a predominantly fibrous stroma with focal myxoid change, admixed with scattered mature adipocytes. Mitotic activity was low and necrosis was absent. The tumor cells co‑expressed CD34, desmin, estrogen receptor, and progesterone receptor, retained RB1 expression, and were negative for smooth muscle actin, h‑caldesmon, and cytokeratin. Targeted RNA sequencing identified an in‑frame RNF19A(ex1)::PLAG1(ex3) fusion, which was confirmed by reverse transcription polymerase chain reaction and Sanger sequencing. Fluorescence in situ hybridization demonstrated PLAG1 gene rearrangement and also revealed PLAG1 copy number gain. Diffuse nuclear PLAG1 expression by immunohistochemistry further confirmed the molecular findings. The patient is recurrence‑free at 4 months of follow‑up. This distinctive combination of myofibroblastic features, focal adipocytic differentiation, and PLAG1 rearrangement has not been previously reported in a uterine mesenchymal tumor. This case broadens the histologic and molecular genetic spectrum of PLAG1-rearranged uterine mesenchymal tumors and highlights the diagnostic value of molecular testing in unusual uterine spindle cell lesions. - Source: PubMed
Publication date: 2026/05/25
Yin XiaonaWang HeliXu JiayunFang RongZhao Ming - RNF19A is a poorly characterized ring between ring finger (RBR) E3 ubiquitin ligase that acts as a tumor suppressor in bladder cancer, whereas as an oncogene in non-small cell lung cancer. However, the biological functions of RNF19A in gastric cancer cells and whether RNF19A targets other substrates involved in gastric cancer progression remain unclear. Here, we report that RNF19A expression is elevated in gastric cancer tissues and is indicative of poor prognoses. - Source: PubMed
Publication date: 2026/01/07
Cheng YuLi ShuaiLi XiangXue JingHao MeilingZhou LiliLuo YuZhang YiJunLi Yuhong - Although immune checkpoint blockade (ICB) is an effective therapeutic strategy, tumor intrinsic resistance to ICB limits its benefits for a majority of cancer patients. Via multiple in vivo screens, we identified RNF19A as an immune suppressor. RNF19A suppresses ICB efficacy by ubiquitinating cGAS to inhibit the release of type I interferon. Surprisingly, UBE2L6, originally identified as a critical interferon-induced ISG15-conjugating E2 enzyme for ISGylation, functions as the major ubiquitin-conjugating E2 enzyme to enable RNF19A-driven cGAS ubiquitination. Further, RSK1 phosphorylates UBE2L6 to switch its substrate from ISG15 to ubiquitin, thus converting it from an immune response enhancer to a suppressor. In xenograft models, targeting RSK1 or blocking UBE2L6 phosphorylation improves immune response. In patient tissues, the levels of UBE2L6 and its phosphorylation correlate with poor immune infiltration, limited immune and radiation therapy response, and prognosis. Taken together, we demonstrate that RSK1-mediated UBE2L6 phosphorylation and UBE2L6-RNF19A-driven cGAS ubiquitination lead to tumor-intrinsic immune suppression. - Source: PubMed
Publication date: 2025/09/22
Peng QiaoChen YunfeiZhao JunmiaoZhu JialiZhuo HuiminZheng YuyingWeng LinjunRuiqing He Tian EnmingZhang YiyiChen XianfeiLiu YuweiTan XiaoWu LeileiTian HonglingLiu JingxinZhang JiawenZhang WenhuiZhang HepingZhang BilongShan ZezhiZhang HaoyuGao YaohuiMeng TongZhao LinlinFang LinShen BingFeng YueLiu LeiYang HuiFang LanWang Ping - Ubiquitin is a small, highly conserved protein that acts as a posttranslational modification in eukaryotes. Ubiquitination of proteins frequently serves as a degradation signal, marking them for disposal by the proteasome. Here we report a novel small molecule from a diversity-oriented synthesis library, BRD1732, that is directly ubiquitinated in cells, resulting in dramatic accumulation of inactive ubiquitin monomers and polyubiquitin chains, which causes broad inhibition of the ubiquitin-proteasome system. Ubiquitination of BRD1732 and its associated cytotoxicity are stereospecific and dependent on two homologous E3 ubiquitin ligases, RNF19A and RNF19B, and their shared E2 conjugating enzyme, UBE2L3. Our finding opens the possibility for indirect ubiquitination of a target through a ubiquitinated bifunctional small molecule and more broadly raises the potential for posttranslational modification in trans. - Source: PubMed
Publication date: 2025/08/21
Li WeichengGarcia-Rivera Enrique MMitchell Dylan CChick Joel MMaetani MicahBhadoria RohitKnapp John MMisu RyosukeMatthews Geoffrey MShirasaki Ryosukede Matos Simoes RicardoViswanathan VasanthiPulice John LRees Matthew GRoth Jennifer AGygi Steven PMitsiades Constantine SKadoch CigallSchreiber Stuart LOstrem Jonathan M L