Ask about this productRelated genes to: RHOA antibody
- Gene:
- RHOA NIH gene
- Name:
- ras homolog family member A
- Previous symbol:
- ARH12, ARHA
- Synonyms:
- RhoA, Rho12, RHOH12
- Chromosome:
- 3p21.31
- Locus Type:
- gene with protein product
- Date approved:
- 1990-03-19
- Date modifiied:
- 2019-04-23
Related products to: RHOA antibody
Related articles to: RHOA antibody
- Phosphodiesterase 4 (PDE4) enzymes regulate intracellular cyclic adenosine monophosphate (cAMP) and thereby influence multiple cancer-relevant processes. Metastasis and angiogenesis, which rely on coordinated cytoskeletal remodelling and integrin-mediated signalling are key determinants of cancer progression. We previously reported KTX207, a cereblon-based proteolysis-targeting chimera (PROTAC) that selectively degrade PDE4D shortforms and suppresses tumour cell proliferation. - Source: PubMed
Publication date: 2026/05/21
Zorn AlinaZhao YiGiblin AoifeBelka IgorTorres EduardoSwindlehurst CathyChan KyleStirling DavidBaillie George SSin Yuan Yan - Carbonized nanomaterials possess diverse functional moieties that complicate biomedical use but also offer multi-target therapeutic potential. Here, carbonized nanogels (CNGs) were synthesized from sodium alginate (Alg) via mild pyrolysis, yielding ultrasmall graphene-like domains embedded in a crosslinked nanogel matrix with abundant bioactive moieties. These features endowed Alg-CNGs with antioxidative, anti-inflammatory, and membrane-perturbing properties, enabling modulation of cellular signaling relevant to cancer progression. In vitro, Alg-CNGs suppressed epithelial-mesenchymal transition in 4 T1 triple-negative breast cancer cells by increasing E-cadherin and decreasing N-cadherin and vimentin, thereby attenuating EMT-associated motility as evidenced by reduced migration/invasion and marked perturbation of actin organization and adhesion-related dynamics. Quantitative proteomics coupled with Ingenuity Pathway Analysis further indicated attenuation of metastasis-associated networks, including RhoA/Rac1/Cdc42-integrin signaling and PI3K/AKT, PTEN, and ERK/MAPK pathways. In an orthotopic breast cancer model, Alg-CNGs accumulated selectively in tumors and reduced pulmonary metastasis by 90% without systemic toxicity. Structure-function analysis indicated that phenolic, lactone, and quinone moieties on the CNGs may contribute to regulating oxidative stress, actin remodeling, and receptor-mediated signaling. These findings demonstrate that Alg-CNGs exert "cocktail-like" multifunctional actions, positioning carbonized polysaccharide-based nanomaterials as a promising and versatile therapeutic strategy against metastatic cancer. - Source: PubMed
Publication date: 2026/05/20
Wang Chen-YowMao Ju-YiUnnikrishnan BineshChan Kai-MinSarkar SaumyadipLin LinHsu Pang-HungHuang Yu-FenHarroun Scott GHuang Chih-ChingChen Shiow-Yi - Subretinal fibrosis (SRF) is a critical end-stage feature of neovascular age-related macular degeneration (nAMD) with limited treatment options. However, the pathological mechanism during the transformation of choroidal neovascularization (CNV) into SRF remains unclear. - Source: PubMed
Publication date: 2026/05/19
Lin RuoyiCai ZixinZheng TianyuWu YanXu ZihangLuo YunhongLiang FengZhu ManhuiYu JingCai Wenting - The mechano-sensitivity of bone marrow-derived macrophages (BMDMs) is crucial for bone remodeling. In addition to force strength, BMDMs also showed a force-direction-dependent response. However, how anisotropic force regulates the function and differentiation of BMDMs is still under debate. Herein, a single-cell-level force-application system was developed to manipulate cells in a noncontact model based on biospecific magnetic microbeads. By adjusting the magnetic field parameters, the microbeads attached to the cell surface can generate controllable forces with specific directions. BMDMs exhibited differential responses to tensile and compressive forces regarding cell spreading. Surprisingly, although less potent than tensile force, compressive force demonstrated a significant suppressive effect on the osteoclast differentiation of BMDMs. The results suggest that this cellular behavior results from distinct pathways through which BMDMs sense and transduce tensile and compressive forces. BMDMs sense tensile force signals through α5β1 integrin and transduce them via the Rac-pPAK pathway. Compressive force, however, initially activates αvβ3 integrin on BMDMs, leading to signal transduction through the RhoA/ROCK-pMLC signaling axis that regulates BMDM differentiation. Furthermore, both in vitro coculture and in vivo subcutaneous ectopic osteogenesis studies suggest that tensile and compressive forces not only individually regulate the fate specification of BMDMs and BMSCs but also simultaneously mediate the crosstalk between these cell types. These findings provide novel insight into the mechanoresponsive mechanisms of BMDMs, deepening our understanding of mechanically induced bone remodeling. - Source: PubMed
Publication date: 2026/05/20
Xiao LiLi JiaLi QunWu DanCao ShuqinWang YuanYu Leixiao - Quantitative real-time PCR (RT-qPCR) is a powerful method for gene expression analysis, but its accuracy critically depends on the selection of stable reference genes for normalization. In non-model organisms such as sponges (phylum Porifera), this task is complicated with high biological variability, seasonal fluctuations, and limited molecular resources. In this study, we first identified and validated candidate reference genes in the calcareous sponge Leucosolenia corallorrhiza. Seven commonly used housekeeping genes (ACT1, GAPDH, RPL13A, HPRT1, RPS3A, TBP, LMN1) were selected based on available transcriptomic and genomic data, and their expression stability was evaluated using geNorm, NormFinder, BestKeeper, and RefFinder under physiological conditions and during tissue regeneration. RPL13A, ACT1, and GAPDH were identified as the most stable reference genes in L. corallorrhiza. To assess whether reference genes identified in L. corallorrhiza can be applied more broadly, we extended the analysis to three phylogenetically and ecologically distinct sponge species: Halisarca dujardinii (marine demosponge), Ephydatia fluviatilis (freshwater demosponge), and Lycopodina hypogea (carnivorous marine demosponge). The same panel of candidate genes was evaluated in all species using the same analytical approaches. Although similar subsets of genes (including RPL13A, ACT1, and GAPDH) consistently ranked among the most stable candidates, no single gene exhibited universal stability across all species. Pairwise variation analysis indicated that the use of two reference genes is sufficient for accurate normalization, while the geometric mean of three top-ranked genes further improves reproducibility and reduces the risk of false-positive results, as demonstrated performing RHOA normalization. Overall, our results demonstrate that reference gene stability in sponges is species-specific and cannot be reliably predicted based on ecological or phylogenetic grouping alone. At the same time, we define a robust panel of candidate reference genes that can serve as a starting point for RT-qPCR studies in Porifera, provided that species- and condition-specific validation is performed. Our study also highlights that technical challenges inherent to research on non-model organisms must be carefully considered in the design of future studies. - Source: PubMed
Publication date: 2026/05/20
Skorentseva Kseniia VMelnikov Nikolai PEreskovsky Alexander VLavrov Andrey ISaidova Aleena A