Ask about this productRelated genes to: SERPINE1 antibody
- Gene:
- SERPINE1 NIH gene
- Name:
- serpin family E member 1
- Previous symbol:
- PLANH1, PAI1
- Synonyms:
- PAI
- Chromosome:
- 7q22.1
- Locus Type:
- gene with protein product
- Date approved:
- 1986-01-01
- Date modifiied:
- 2019-04-23
Related products to: SERPINE1 antibody
Related articles to: SERPINE1 antibody
- Myocardial fibrosis (MF), a common pathological consequence of cardiovascular diseases, compromises cardiac function and elevates the risk of heart failure and arrhythmias. Considering the limited therapeutic options, this study explored the antifibrotic potential of Obacunone (OB), focusing on its links to lipid metabolism pathways. Potential OB targets, MF-associated genes, and lipid metabolism-related genes were curated from databases and literature. Transcriptomic datasets were analyzed to identify differentially expressed genes (DEGs) in MF, and lipid metabolism-related DEGs (LMDEGs) were subsequently intersected with these target sets to identify OB-associated targets. These core targets were further investigated via protein-protein interaction (PPI) network, consensus clustering, functional enrichment, and molecular docking analysis. Key findings were validated via western blotting. Intersection analysis identified ten LMDEGs associated with OB. Moreover, PPI network analysis highlighted a subnetwork of seven strongly interacting targets-CYP19A1, STAT3, LGALS3, PDGFRA, SCN5A, SLC9A1, and SERPINE1. Functional enrichment indicated OB's involvement in the epidermal growth factor receptor (EGFR), advanced glycation end-product-receptor for advanced lycation end-products (AGE-RAGE), and hypoxia-inducible factor-1 (HIF-1) signaling pathways. Furthermore, consensus clustering revealed distinct subtypes of MF. Molecular docking confirmed strong binding affinities between OB and core targets. In vivo, OB attenuated fibrosis, downregulated SLC9A1 and SERPINE1 expression, and upregulated CYP19A1, LGALS3, PDGFRA, and SCN5A. This integrated study demonstrates that OB exerts antifibrotic effects by regulating lipid metabolism-related genes and pathways. The identification of MF subtypes supports personalized therapy, positioning OB as a promising candidate for MF treatment. - Source: PubMed
Publication date: 2026/05/28
Liang GuanfengLin YongchunLi LipingCai JingyueLiu Xiaoping - Sex differences in calcific aortic valve disease (CAVD) progressing to aortic valve stenosis have been documented clinically, but the underlying cellular mechanisms that drive sex-dependent fibrocalcification in aortic valve tissue remain poorly understood. - Source: PubMed
Publication date: 2026/05/28
Baddour TaliaNinh Van KGorashi Rayyan MPeña ReneKing Kevin RAguado Brian A - Microcystin-LR (MC-LR) is a potent hepatotoxin that has been shown to cause liver damage even at doses lower than the established Low Observable Adverse Effect Level (LOAEL) of 200 μg/kg in animal models. We have previously observed that low-dose exposure to MC-LR in animals with diet-induced Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD) and subsequent treatment with antioxidants like N-acetylcysteine (NAC) and the Na/K ATPase-Src kinase inhibitor pNaKtide significantly alleviated hepatic infiltration of immune cells, downregulated markers of inflammation and hepatotoxicity, increased the breakdown of the toxin molecule, and restored phase I and II drug metabolism pathways, including the glutathione pathway. Because the liver is composed of heterogeneous cell types, this study aimed to determine the specific role of hepatocytes in the uptake and metabolism of MC-LR, especially in the setting of MASLD. To address this, we used two well-established hepatocyte cell lines-AML-12 murine hepatocytes and human Hep3B hepatocytes. Preliminary dose comparison studies with AML-12 cells showed that MC-LR at 10 μM concentration showed a significant upregulation in the genetic expression of the markers of hepatotoxicity- ( ≤ 0.01) and ( ≤ 0.0001)-in comparison to Vehicle. Treatment with pNaKtide (1 µM) and/or NAC (10 mM) in the presence of MC-LR significantly reduced the expression of both ( ≤ 0.0001) and ( ≤ 0.01 and ≤ 0.0001, respectively). To model steatotic hepatocytes characteristic of the MASLD phenotype, Hep3B hepatocytes were first treated with 500 µM of oleic acid (OA) before exposing them to the toxin in the presence and absence of antioxidants. MC-LR exposure, induced markers of inflammation and hepatotoxicity to be elevated significantly in the presence of OA as compared to MC-LR exposure alone. This elevation of the genetic markers of inflammation and hepatotoxicity was significantly attenuated on treatment with pNaKtide (1 µM) and NAC (10 mM). Quantification of human SERPINE1 (PAI1) and 8-OHdG, a stable marker of oxidative stress, in the spent media of Hep3B cells corroborated the trends observed in the genetic markers of hepatotoxicity. These observations support the central role that hepatocytes play in the uptake and metabolism of MC-LR, which is complicated by the presence of MASLD-like conditions and can help in the development of future therapeutic strategies. - Source: PubMed
Publication date: 2026/04/29
Lad ApurvaKindle JasonHegde PrajwalKleer Gabriel GKleinhenz Andrew LBirbeck Johnna AWestrick JudyPeraino Nicholas JHinds Terry DPurandare NeerajaFribley Andrew MHaller Steven TKennedy David J - Host genetic factors may contribute to COVID-19 severity. To identify genetic variants influencing COVID-19 severity progression, whole-exome sequencing was performed, followed by an exome-wide association study on 191 hospitalized patients categorized into three severity groups. The analysis identified nine loci surpassing the suggestive significance threshold (p ≤ 3 × 10): rs2180196 (β = 2.09, TGM1-RABGGTA), rs2236232 (β = -1.37, PLEKHH1), rs7886938 (β = 1.66, FMO6P), rs2929047 (β = -1.16, XKR4), rs9841237 (β = 1.21, SI), rs34357454 (β = 1.92, NID2), rs1005887 (β = 1.28, DUSP18), rs2281880 (β = -1.11, SUFU), and rs73061693 (β = 1.66, TULP2). Furthermore, binomial exact test identified 218 SNPs in COVID-19-related genes with significant allele frequency differences between severity groups, including 61 novel variants. Of those 218, co-occurring SNP pairs were identified within specific severity groups, including pairs in JAK1-PIK3R2 in critical patients and eight pairs in genes IL6, SERPINE1, COL5A1, MAPK14, FREM1, C7, GLS, ATP5PO, and RPL7 in severe patients. Clinical data analysis confirmed known risk factors like older age in men and specific comorbidities and revealed distinct therapeutic and biochemical profiles across severity groups. This study confirms known genetic risks and reveals novel variants, providing a more detailed basis for understanding COVID-19 pathogenesis and for developing genetic diagnostic and prognostic biomarkers. - Source: PubMed
Repušić DorisKorolija MarinaLivun AnaSvaguša TomoBelužić RobertTomaić VjekoslavKarlić RosaVlahoviček Kristian - High-altitude hypoxic adaptation in mammals involves complex molecular mechanisms, with non-coding RNAs (ncRNAs) increasingly reported to participate in hypoxia-related regulation. However, the contribution of circRNAs in cardiac adaptation to chronic hypoxia remains largely unexplored. This study performed an integrative competitive endogenous RNA (ceRNA) analysis to investigate circRNA-mediated regulatory networks in the hearts of Tibetan pigs and Yorkshire pigs maintained under high- and low-altitude conditions, using four comparison groups (TH, TL, YH, and YL). Using Ribo-Zero RNA sequencing, we identified 961 circRNAs in heart tissues, with 358 differentially expressed circRNAs (DE-circRNAs) detected across the four groups. Functional enrichment analysis revealed that their host genes were associated with hypoxia-related pathways, including HIF-1, VEGF, AMPK, and autophagy, critical for energy metabolism and mitochondrial function. A HIF-1-specific ceRNA network was constructed, identifying key axes including circDUSP16-ssc-miR-671-5p-, circTLK1-ssc-miR-331-3p-, and circTLK1-novel-miR-624-. JASPAR analysis predicted potential HIF-1α binding sites in the promoters of , , and , supporting their regulatory roles. These findings provide a transcriptomic overview of circRNA expression patterns in pig heart tissues under different altitude conditions and prioritize candidate ceRNA relationships for further functional investigation. - Source: PubMed
Publication date: 2026/05/14
Li PanCheng WeiShang PengTao ZhuZhang HaoZhang Bo