Ask about this productRelated genes to: PAFAH1B3 antibody
- Gene:
- PAFAH1B3 NIH gene
- Name:
- platelet activating factor acetylhydrolase 1b catalytic subunit 3
- Previous symbol:
- -
- Synonyms:
- -
- Chromosome:
- 19q13.1
- Locus Type:
- gene with protein product
- Date approved:
- 1998-04-03
- Date modifiied:
- 2015-11-16
Related products to: PAFAH1B3 antibody
Related articles to: PAFAH1B3 antibody
- Assisted reproductive technologies, such as fertilization, remain inefficient in camelids, largely due to gaps in understanding the molecular interactions that regulate sperm capacitation. Fertilization requires not only viable spermatozoa but also the precise modulation of capacitation by the peri-ovulatory microenvironment, including follicular fluid (FF) and oviductal fluid (OF). In this study, spermatozoa were incubated in Fert-TALP medium supplemented with FF or OF, and both functional outcomes and proteomic remodeling were assessed. Sperm treatments were evaluated in five independent biological replicates per individual (three individuals), with triplicate proteomics performed. FF ( = 20) was collected from pre-ovulatory follicles (7-9 mm) and OF ( = 10) from the corresponding ipsilateral oviducts, thereby reflecting the environment encountered by sperm in the female reproductive tract following mating. Incubation with FF enhanced progressive motility by 72%, rapid progressive motility by 169%, viability by 30%, and acrosome responsiveness by 30%, and was associated with a proteomic shift involving ~12% of proteins ( < 0.05). These included factors implicated in zona pellucida binding (LYPD4, PGK1, ANXA2, and TCP1 complex members) and galactose metabolism (MAOA, AKR1B1, GLA, and HK1). The enriched processes included glycolysis/gluconeogenesis, cytoskeletal reorganization, and protein maturation, all consistent with sperm capacitation. By contrast, sperm incubated with OF showed an underrepresentation of capacitation-related pathways, including the proteasome complex, sperm fibrous sheath, and TCA cycle. Moreover, the OF proteome ( = 2) revealed decapacitation-associated factors such as PEBP1 and PAFAH1B3, which likely stabilize membranes and delay premature capacitation. Together, these findings demonstrate complementary yet contrasting roles of FF and OF in modulating sperm physiology: FF acting as a capacitating medium, and OF providing a stabilizing environment. This study presents the first partial proteome of capacitated alpaca sperm together with matched reproductive fluids, providing mechanistic insights with direct implications for improving assisted reproduction in camelids. - Source: PubMed
Publication date: 2025/11/06
Torres Hualla Edith AMartiarena AlbaBuglio Ballesteros María GabrielaMedina Rojas Maribel FortunataRivera Chino CristianGandarillas Espezua DanielArgañaraz Martin E - Bladder cancer (BCa), the most prevalent malignancy of the urinary system, is strongly associated with environmental factors including smoking and industrial chemical exposure. Although previous studies have explored the relationship between various environmental pollutants and BCa mechanisms, there is still a need for an in-depth analysis of the specific effects and particular roles of these pollutants. This study investigates the molecular mechanisms linking environmental pollutant exposure to bladder carcinogenesis through a multi-omics integrative analysis that systematically elucidates the environment-gene interaction network. By integrating transcriptomic data from multiple BCa cohorts with the Comparative Toxicogenomics Database (CTD) gene-chemical-disease network, we identified 168 environment-responsive differentially expressed genes (DEGs). Subsequent Mendelian Randomization (MR), Summary-data-based MR (SMR), and colocalization analyses revealed three causal hub genes (CTSK, GSTM5, and PAFAH1B3; PP4 > 0.70) and associated them with 34 high-risk environmental pollutants. Molecular docking demonstrated strong binding affinities between these hub genes and more than ten pollutants, with bisphenol A, sodium arsenite, and tetrachlorodibenzodioxin (TCDD) differentially regulating distinct targets: significantly suppressing tumor-suppressor genes CTSK (OR = 0.91, p = 0.004) and GSTM5 (OR = 0.77, p < 0.001) while upregulating the oncogenic factor PAFAH1B3 (OR = 2.11, p < 0.001). Single-cell RNA sequencing (scRNA-seq) analysis and Human Protein Atlas (HPA) database validation confirmed tissue-specific expression patterns of these hub genes in bladder tissues. Our findings establish a comprehensive evidence chain connecting "environmental pollutant exposure - gene interaction - bladder carcinogenesis," providing novel biomarkers and preventive targets for molecular subtyping and precision prevention of environmentally associated BCa. - Source: PubMed
Publication date: 2025/10/18
Zhu GuangqiangTan ChunlinLi Yugen - High-altitude environments are characterised by extreme conditions, including hypoxia, low temperatures, and intense ultraviolet radiation. Mammals inhabiting these environments have evolved unique adaptive mechanisms, the study of which elucidates survival strategies and evolutionary pathways under extreme conditions. Understanding how native high-altitude animals respond to such environments is highly important. This study investigated the high-altitude adaptation mechanisms of the five-toed jerboa (Orientallactaga sibirica) distributed in Qinghai Province (4229 m) and Hebei Province (498 m), China, through comparative transcriptomic analysis of heart, lung, and kidney tissues. The results revealed greater mRNA transcriptional differences in the lung tissue than in the heart and kidney tissues of high-altitude jerboas, indicating heightened lung sensitivity to high-altitude conditions. In lung tissue, high-altitude jerboas show differential expression of genes related to the Complement and Coagulation cascades, Heme binding, Oxidation-reduction process (such as MASP1, A2M, SERPING1, CD55, FGA, C5AR1, and KNG1), which may be associated with modulating immune functions to mitigate hypobaric hypoxia, intense radiation, and cold-induced damage and reducing thrombosis and inflammation risks. Heart tissue exhibits differential expression of Oxidative phosphorylation and Lipid metabolism genes (such as NDUFC2, NDUFA3, NDUFS4, COX4I2, PAFAH1B3, SGMS2 and PPAR2B), which may help maintain energy equilibrium under hypoxic and cold challenges. Kidney tissue exhibits differential enrichment of pathways such as arachidonic acid metabolism and steroid hormone biosynthesis mediated by genes including CYP4A11, CYP2C29, GPX2, PTGDS, CBR1, and UGT2B31, which may help coordinate vascular regulation, immune response, and oxidative balance to maintain systemic homeostasis. These pathways and genes are differentially enriched and expressed between high-altitude and low-altitude five-toed jerboas, which may be candidates for further functional studies of plateau environmental adaptability. Our findings provide candidate genes and pathways for intraspecies adaptations across microenvironments and highlight the need for further functional validation. - Source: PubMed
Publication date: 2025/10/07
Sun Shan-ShanZhang Hao-TingYan Hai-WenKang Xiao-YuBuren Qi-QigeWang Qian-ChengMing MingFeng Jie-RuZhu NaLi XinLing YuZhang DongWu Xiao-DongYuan ShuaiFu He-Ping - Recent evidence highlights the role of extrachromosomal circular DNAs (eccDNAs) in cancers. However, reports regarding its role in hepatocellular carcinoma (HCC) are infrequent. The abundance of eccDNAs from five HCC/adjacent tissue pairs was explored using Circle-Sequencing. eccDNA PAFAH1B3 was selected as one of the objects. The effect of eccDNA PAFAH1B3 on HCC progression was determined using EdU, Transwell, and apoptosis assays. Additionally, the expressions of eccDNA PAFAH1B3, mRNA PAFAH1B3, and epithelial-mesenchymal transition (EMT)-related markers were determined using RT-PCR and WB. A xenograft tumor model was established to explore the function of PAFAH1B3 in vivo, and EMT-related markers were detected using RT-PCR and IHC analyses. The abundance of eccDNA PAFAH1B3 was significantly increased in HCC cell lines after transfection with eccDNA PAFAH1B3, and promoted the proliferation, migration, and invasion of liver cells while inhibiting apoptosis. The levels of mRNA PAFAH1B3 were also upregulated. Furthermore, intratumoral injection of PAFAH1B3 inhibitor suppressed tumor growth, and PAFAH1B3 knockdown increased and decreased the levels of the E-cadherin and N-cadherin, respectively. Our study findings reveal that eccDNA PAFAH1B3 may promote the occurrence and development of HCC by enhancing the expression of PAFAH1B3 and regulating EMT. - Source: PubMed
Publication date: 2025/09/10
Li DandanSun HuishanWang YingjieYin YicongZhu YingQian XiaWang ShanshanZhang LonghaoZhao HaitaoQiu Ling - For over three decades, intracerebroventricular streptozotocin (STZ-icv) has been a key non-transgenic rodent model of sporadic Alzheimer's disease. Traditionally used in rats, its use in mice is growing due to practical advantages. While factors like strain, sex, vehicle, age, and dosage are well studied in STZ-induced diabetes models, their impact on STZ-icv-related cognitive deficits remains poorly understood. To address this gap, we examine species-specific sensitivity to STZ-icv in two widely used rodent strains (Wistar Han rats and C57BL/6 mice) motivated by the finding that, despite reliably inducing cognitive deficits in rats for over 30 years in our lab, the same STZ-icv procedure yields inconsistent results in C57BL/6 mice. To explore this phenomenon, we first constructed a series of allometric models to assess how different STZ-icv concentrations affect predicted exposure in rats and mice. Next, we provide behavioral and immunohistochemical evidence that C57BL/6 mice exhibit resistance to STZ doses that should achieve sufficient neuronal exposure. Finally, we perform an exploratory multiomic analysis, examining differentially expressed proteins in the hippocampus and prefrontal cortex of STZ-icv-treated rats that also show differential expression between Wistar Han rats and C57BL/6 mice, followed by an investigation of these proteins across independent Alzheimer's disease cohorts. Through this analysis, we identify three understudied proteins-Tagln2, Slc25a3, and Pafah1b3-that may contribute to the differential sensitivity of Wistar Han rats and C57BL/6 mice to STZ-icv and may play a role in the development and progression of Alzheimer's disease. - Source: PubMed
Publication date: 2025/06/01
Homolak JanMarkovic PavelVirag DavorKnezovic AnaOsmanovic Barilar JelenaLoncar AndrijaSalkovic-Petrisic MelitaBabic Perhoc Ana