Ask about this productRelated genes to: DDX28 antibody
- Gene:
- DDX28 NIH gene
- Name:
- DEAD-box helicase 28
- Previous symbol:
- -
- Synonyms:
- MDDX28, FLJ11282
- Chromosome:
- 16q22.1
- Locus Type:
- gene with protein product
- Date approved:
- 2001-12-07
- Date modifiied:
- 2016-10-05
Related products to: DDX28 antibody
Related articles to: DDX28 antibody
- Acute myeloid leukemia (AML) is a heterogeneous malignancy with frequent relapse, driven by intertwined alterations in mitochondrial function, cell cycle control, genome maintenance, and immune evasion. DDX28 is a mitochondrial DEAD-box RNA helicase required for mitoribosome assembly and mitochondrial translation, and has been implicated in bioenergetic regulation in other tumor contexts. Here, we profiled DDX28 expression across AML cohorts using integrated multi-omics resources and evaluated its associations with prognosis, immune microenvironment features, and predicted drug response. Functional annotation, pathway analysis, GSEA, and targeted in vitro assays were used to explore potential mechanisms. High DDX28 expression was associated with inferior survival and higher blast burden. Mechanistically, elevated DDX28 expression was linked to promoter hypomethylation and was positively correlated with the transcription factor THAP11. Transcriptomic signatures in the high DDX28 group were enriched for cell cycle progression and DNA damage repair programs, together with an immune-suppressive landscape characterized by increased regulatory T cells and M2 macrophage signatures. Single-cell RNA-seq analyses further showed DDX28 enrichment in malignant blasts and exhausted or proliferative T-cell states. Consistently, DDX28 knockdown by siRNA in HEL cells reduced proliferation and impaired migration and invasion. Although direct metabolic flux measurements were not performed, the mitochondrial localization of DDX28 and the enrichment of proliferation and repair programs support a model in which DDX28 couples mitochondrial translation with the biosynthetic and genome maintenance demands of rapidly cycling AML cells. Collectively, our findings identify DDX28 as a prognostic indicator and a candidate regulator of malignant and immune states in AML, with potential relevance to therapeutic response. - Source: PubMed
Publication date: 2026/05/05
Wang ZiLiu YunliGuan XinzhuYe MiaoqingXu Xinxin - Mitochondrial ribosome biogenesis depends on RNA helicases such as DDX28, a DEAD-box helicase that plays an essential role during early mitoribosome large-subunit assembly by interacting with 16S rRNA. Here, we demonstrate that the helicase core domain of DDX28 binds sequence and structure specifically to the H88_L stem-loop in 16S rRNA, with the RecA2 domain residue M431 as a key determinant for substrate selectivity. The N-terminal disordered region of DDX28 enhances nonspecific RNA binding but does not contribute to enzymatic activity. Furthermore, DDX28 deficiency disrupts mitochondrial translation, impairs OXPHOS complex assembly, and leads to metabolic dysfunction, including reduced membrane potential, elevated ROS, and suppressed glycolysis. Transcriptomic and metabolomic analyses reveal a compensatory upregulation of ribosome biogenesis genes alongside a dysregulation of the TCA cycle, oxidative phosphorylation, and lipid metabolism. Our integrated structural and functional study establishes DDX28 as an essential factor for mitoribosome assembly with potential links to mitochondrial disorders. - Source: PubMed
Publication date: 2026/03/11
Cui JingLi MeiliWang LeiLi FudongRuan KeLv MengqiShi Yunyu - Hypoxia is a common characteristic of the tumor microenvironment leading to aggressive phenotypes. A major response to hypoxia is through the induction of gene programs by the hypoxia-inducible factors (HIF). Previously, we showed that the DEAD-box RNA helicase DDX28 negatively regulates hypoxic eIF4E2-directed translation through its interaction with HIF-2α. We hypothesized that DDX28 is a tumor suppressor that represses the oncogenic HIF-2α axis. Here, we overexpress DDX28 in MDA-MB-231 breast cancer and U87MG glioblastoma cells that have very low and normal endogenous levels of DDX28, respectively, compared with noncancerous HEK293. We show that DDX28 suppresses cell migration, spheroid growth, and invasion in MDA-MB-231, but not U87MG cells. However, suppression is not through the HIF-2α gene program, but through DDX28 impacting cellular bioenergetics. DDX28 levels altered how cells utilized mitochondrial respiration and glycolysis for ATP generation. Furthermore, the pharmacological inhibition of these processes specifically reversed the effects of DDX28 overexpression. This study shows that low endogenous DDX28 levels promote hypoxic migration, and growth/invasion in three-dimensional structures in cells that have a bioenergetic profile that favors glycolysis such as MDA-MB-231. - Source: PubMed
Publication date: 2025/06/25
Bebenek OliviaPascetta Sydney ASteed JoshuaMizzoni MorganKellington Alexandria TBarnes Margaret KOsorio-MacCready TessUniacke James - Preeclampsia, a hypertensive disorder during pregnancy affecting 2-8% of pregnancies globally, remains a leading cause of maternal and fetal morbidity. Current diagnostic reliance on late-onset clinical features and suboptimal biomarkers underscores the need for early molecular predictors. Ribosome biogenesis, critical for cellular homeostasis, is hypothesized to drive placental dysfunction in PE, though its role remains underexplored. - Source: PubMed
Publication date: 2025/06/09
Chen JingjingZhang DanZhu ChengxiuLin LinYe KejunHua YingPeng Mengjia - Bacterial strains, designated DD3 and DDX28, were isolated from field soil in Japan. The strains had the ability to use 2,4-dichlorophenoxyacetic acid as the sole carbon source. They were Gram-reaction-negative, oxidase-positive, weakly catalase-positive, aerobic and non-spore-forming. Their cells were rod-shaped and often lacked flagella, but some exhibited motility due to the presence of one or two polar flagella. The genomic DNA G+C content was 58.8 mol%, and the major cellular fatty acids (>10% of the total fatty acids) were summed feature 8 (C and/or C ), C and C cyclo. Phylogenetic analyses based on gene sequences and phylogenomic analysis using whole-genome sequences confirmed that the strains belong to the genus ; however, their phylogenetic position did not match that of any known species of this genus. Comparative studies of the average nucleotide identity and digital DNA-DNA hybridization with closely related species revealed values lower than the thresholds used for prokaryotic species delineation (95-96 and 70%, respectively), with the highest values observed for ATCC 49717 (79.92 and 21.5%, respectively). Phenotypic characteristics, cellular fatty acid composition and specific metabolic processes and biosynthetic gene clusters could differentiate the strains from their closest relatives. Our phenotypic, chemotaxonomic and genotypic data indicate that DD3/DDX28 constitute a novel species, for which we propose the name sp. nov., with DD3 (MAFF 311804=ICMP 25015) as the type strain. - Source: PubMed
Sawada HiroyukiSakai YorikoTakashima YusukeNaito KenHorita MitsuoSatou Mamoru