Ask about this productRelated genes to: LARP4 antibody
- Gene:
- LARP4 NIH gene
- Name:
- La ribonucleoprotein domain family member 4
- Previous symbol:
- -
- Synonyms:
- PP13296
- Chromosome:
- 12q13.12
- Locus Type:
- gene with protein product
- Date approved:
- 2005-06-16
- Date modifiied:
- 2019-02-21
Related products to: LARP4 antibody
Related articles to: LARP4 antibody
- Cytoplasmic poly(A)-binding protein (PABPC1) binds the mRNA poly(A) tail via its RNA recognition motifs (RRMs) and recruits factors involved in mRNA metabolism through its C-terminal MLLE domain. The MLLE domain recognizes short PAM2 motifs found in a wide range of regulatory proteins known as PAM2-containing proteins (PACs). Members of the La-related protein (LARP) family, specifically LARP1 and LARP4, which are involved in poly(A) tail protection, contain atypical PAM2 sequences that diverge from the canonical consensus yet retain MLLE binding. The molecular basis of this interaction is not fully understood for LARP1. Here, we combined nuclear magnetic resonance (NMR) spectroscopy, isothermal titration calorimetry (ITC), and AlphaFold3 modeling to characterize the interaction between the LARP1 PAM2 motif and the MLLE domain of PABPC1. NMR chemical shift perturbations and heteronuclear NOE analyses defined the MLLE-binding segment, i.e. PAM2 motif, within LARP1. Mutagenesis studies and ITC confirmed the essential role of phenylalanine 496 for MLLE binding. AlphaFold3 modeling corroborated these findings with the prediction of a single turn α-helix in the PAM2 motif. Together, these results define a non-canonical PAM2-MLLE interaction and reveal unexpected plasticity in the recognition of PAM2 motifs by the MLLE domain. - Source: PubMed
Publication date: 2026/02/26
Behvarmanesh AliKozlov GuennadiSprules TaraGehring Kalle - The utility of messenger RNA (mRNA) as a therapeutic modality has been widely demonstrated with the containment of COVID-19, yet the decisions in sequence design in the untranslated regions (UTRs) remain largely unexplored, especially in preclinical models. Here, we focus on the 5' UTR of mRNA and discover sequences that improve therapeutic potential in mouse models of aging and obesity. Bioinformatic analysis of RNA sequencing (RNA-seq), single-cell RNA-seq, ribosome profiling, and crosslinking and immunoprecipitation followed by sequencing data revealed that ribosomal protein (RP) mRNAs are abundant and ubiquitous but undergo distinct translational regulation by LARP1 and LARP4. Of 11 RP mRNAs, we find that the 5' UTRs of RPL18, RPL35, and RPS9 improve the protein output of synthetic mRNAs in human and mouse cells. Investigation of mutant 5' UTRs indicates that this improvement is independent of its terminal oligopyrimidine motif but strong in cells with high levels of reactive oxygen species. In aged mice and mice receiving a high-fat diet, synthetic mRNAs with the 5' UTR of RPS9 resulted in improved protein expression and enhanced humoral immunity through T helper cell 2 cytokines when encoding viral antigens. Altogether, our results highlight the importance of UTR sequence in expanding the therapeutic potential of synthetic mRNAs for aged individuals and those diagnosed with obesity. - Source: PubMed
Publication date: 2026/01/02
Yoon SubinCho HyeonggonLee JisunHa SunghyunLee SuyeonLee Yu-SunCho YoungranHa DahyeonOh AyoungLee SeonghyunJeong DaheeCho JunPark Sang-InNam Jae-HwanLee Young-Suk - Genitourinary malignancies represent a major global health burden, with rising incidence and mortality rates, particularly in China. Comprehensive genomic profiling in Chinese patients remains limited. This study aimed to characterize the mutational landscape of genitourinary cancers to identify potential biomarkers for improved diagnosis and treatment. - Source: PubMed
Publication date: 2025/12/05
Lu XinZhang XiangCheng ShaochenNing WeiqiangZhao ShuhuaShao YuanChen WeiZhang Jun - Naive T cells are maintained under a quiescent state, and their exit from quiescence is a hallmark of antigen stimulation. Here we identify the RNA binding protein La-related protein 4 (LARP4) as an important checkpoint regulator of quiescence exit in naive CD4 T cells. Conditional knockout of LARP4 in naive CD4 T cells leads to an enhanced quiescence state and/or dampened quiescence exit due to altered stability of several messenger RNAs important for T-cell activation. The differentiation of naive CD4 T cells into helper T-cell subsets is also impaired after conditional knockout, leading to ameliorated autoimmune and allergic responses. Lastly, we design a peptide inhibitor of LARP4 (LIPEP), and treatment with LIPEP could perfectly mimic LARP4 deficiency and alleviate the severity of autoimmune and allergic diseases in the corresponding mouse models. Our study reveals a link between RNA stability and CD4 T-cell homeostasis/adaptive activation, highlighting the potential of LARP4 as a preventative and therapeutic target for autoimmune and allergic diseases although at quite high doses. - Source: PubMed
Publication date: 2025/10/16
Zhou JianYang DiHan ChaoDong HuiWang ShufengLi XiangHu JunWang CuiLuo JieWei ZhiyuanLiu TaipingXu ShuaiXu ChenZhang YiweiWang XianDeng YuanyuLi BaiqingMao RuihanZhang MingyangSun YiZhou XinyuanYe LilinNi BingZhu JunLi JuanZhang JingboZhao TingtingChen XiangmeiLin RongZhang YiWu YuzhangTian Yi - - Source: PubMed
Liu YiNi HaochenLi JieYang JingSekielyk IvannSnow Bryan EZhang ZihaoZhang FeifanPaul Michael StHan JinyiKates MeghanLiu ShaofengZhang YaweiHuang ZuruiXu YinSaibil Samuel DMak Tak WHan DaliXu Meng Michelle