Ask about this productRelated genes to: PAPOLB antibody
- Gene:
- PAPOLB NIH gene
- Name:
- poly(A) polymerase beta
- Previous symbol:
- -
- Synonyms:
- PAPT
- Chromosome:
- 7p22.1
- Locus Type:
- gene with protein product
- Date approved:
- 2001-06-25
- Date modifiied:
- 2015-11-06
Related products to: PAPOLB antibody
Related articles to: PAPOLB antibody
- MicroRNAs (miRNAs) play an important role in the occurrence of non-obstructive azoospermia (NOA). Nevertheless, there is still a lack of research on the molecular mechanisms by which miRNAs regulate target genes to mediate NOA at present. In this study, we obtained NOA-related miRNA datasets from the GEO database and applied differential expression matrices combined with weighted correlation network analysis (WGCNA) and LASSO regression to identify four key miRNAs. Based on the miRDB database, the target genes of these miRNAs were predicted and intersected with the differentially expressed genes (DEGs) in the NOA transcriptome datasets. This intersection resulted in the identification of 18 DEGs. The spermatogenesis score model revealed a significant positive correlation between the overall expression level of these 18 DEGs and spermatogenesis scores, suggesting their potential involvement in NOA development. These 18 DEGs were subsequently incorporated into machine learning, leading to the identification of four hub genes with high diagnostic value: , , , and . In the NOA mouse model, and were upregulated, whereas and were downregulated, consistent with the expression trends observed in the NOA datasets. These findings indicate that , , , and may serve as novel biomarkers for NOA, providing a theoretical and experimental foundations for elucidating its mechanisms and improving clinical diagnosis. - Source: PubMed
Li Zhi-HongChen Miao-QiYuan Xiao-JunHuang Hua-JunHuang Wan-TingZhou Piao-YanZeng ChenFeng XunuoYang Luo-YaoHuang Shu-QiangTan Cui-YuChen Cai-RongYan Qiu-Xia - Genetic progress for fertility and reproduction traits in dairy cattle has been limited due to the low heritability of most indicator traits. Moreover, most of the quantitative trait loci (QTL) and candidate genes associated with these traits remain unknown. In this study, we used 5.6 million imputed DNA sequence variants (single nucleotide polymorphisms, SNPs) for genome-wide association studies (GWAS) of 18 fertility and reproduction traits in Holstein cattle. Aiming to identify pleiotropic variants and increase detection power, multiple-trait analyses were performed using a method to efficiently combine the estimated SNP effects of single-trait GWAS based on a chi-square statistic. - Source: PubMed
Publication date: 2022/04/28
Chen Shi-YiSchenkel Flavio SMelo Ana L POliveira Hinayah RPedrosa Victor BAraujo Andre CMelka Melkaye GBrito Luiz F - BNC1 is a transcription factor that is crucial for spermatogenesis and male fertility, although the underlying mechanism remains unclear. To study BNC1's specific role in spermatogenesis, we characterized a previously developed mouse model carrying a truncating mutation in Bnc1 (termed Bnc1 for heterozygotes and Bnc1 for homozygotes) and found that the mutation decreased BNC1 protein levels and resulted in germ cell loss by apoptosis. Given that loss of functional Bnc1 is known to result in decreased expression of the spermatogenesis genes Ybx2 and Papolb, we aimed to explore whether and how BNC1 promotes transcription of Ybx2 and Papolb to mediate its role in spermatogenesis. We confirmed significant reduction in YBX2 and PAPOLB protein levels in testis tissue from Bnc1 and Bnc1 males compared with wild-type mice (Bnc1). Consistently, knockdown of Bnc1 led to downregulation of Ybx2 and Papolb in CRL-2196 cells in vitro. To investigate if BNC1 directly induces Ybx2 and Papolb gene expression, chromatin immunoprecipitation using mouse testicular tissue and luciferase reporter assays in HEK293 cells were used to identify functional binding of BNC1 to the Ybx2 and Papolb promoters at defined BNC1 binding sites. Taken together, this study reveals a mechanism for BNC1's role in spermatogenesis by directly binding to BNC1 binding elements in the promoter regions of both Ybx2 and Papolb and inducing transcription of these important spermatogenesis genes. - Source: PubMed
Publication date: 2020/11/19
Li Jing-YiYing Yan-YunQian Yu-LiChen Jian-PengHuang YunLiu JuanLv Ping-PingLiu Yi-FengHu Xiao-LingSchilit Samantha L PSheng Jian-ZhongHuang He-FengZhang Dan - BACKGROUND The aim of this study was to identify gene signals for lower-grade glioma (LGG) and to assess their potential as recurrence biomarkers. MATERIAL AND METHODS An LGG-related mRNA sequencing dataset was downloaded from The Cancer Genome Atlas (TCGA) Informix. Multiple bioinformatics analysis methods were used to identify key genes and potential molecular mechanisms in recurrence of LGG. RESULTS A total of 326 differentially-expressed genes (DEGs), were identified from 511 primary LGG tumor and 18 recurrent samples. Gene ontology (GO) analysis revealed that the DEGs were implicated in cell differentiation, neuron differentiation, negative regulation of neuron differentiation, and cell proliferation in the forebrain. The Kyoto Encyclopedia of Genes and Genomes (KEGG) database suggests that DEGs are associated with proteoglycans in cancer, the Wnt signaling pathway, ECM-receptor interaction, the PI3K-Akt signaling pathway, transcriptional deregulation in cancer, and the Hippo signaling pathway. The hub DEGs in the protein-protein interaction network are apolipoprotein A2 (APOA2), collagen type III alpha 1 chain (COL3A1), collagen type I alpha 1 chain (COL1A1), tyrosinase (TYR), collagen type I alpha 2 chain (COL1A2), neurotensin (NTS), collagen type V alpha 1 chain (COL5A1), poly(A) polymerase beta (PAPOLB), insulin-like growth factor 2 mRNA-binding protein 1 (IGF2BP1), and anomalous homeobox (ANHX). GSEA revealed that the following biological processes may associated with LGG recurrence: cell cycle, DNA replication and repair, regulation of apoptosis, neuronal differentiation, and Wnt signaling pathway. CONCLUSIONS Our study demonstrated that hub DEGs may assist in the molecular understanding of LGG recurrence. These findings still need further molecular studies to identify the assignment of DEGs in LGG. - Source: PubMed
Publication date: 2019/05/19
Deng TengGong Yi-ZhenWang Xiang-KunLiao Xi-WenHuang Ke-TuanZhu Guang-ZhiChen Hai-NanGuo Fang-ZhouMo Li-GenLi Le-Qun - Approximately 15% of couples are infertile, with half of these cases being due to a male factor. Testis-specific cytoplasmic poly(A) polymerase beta (PAPOLB) is known to be critical for spermatogenesis. In mice, the loss of function of the Papolb gene results in the arrest of spermiogenesis and in male infertility. To analyse the role of the PAPOLB gene in human male infertility, this study investigated the relevance of this gene to human Sertoli-cell-only syndrome (SCOS) with azoospermia. Mutation analysis of the PAPOLB coding region was performed on 139 Japanese patients by PCR and direct sequence analysis. No critical mutations directly causing SCOS were detected, but three single-nucleotide polymorphisms (SNPs; SNP1 (c1101C > T), SNP2 (c1347T > C) and SNP3 (c1903C > A)) were found in the coding region. However, there were no significant associations in the allelic and genotypic distributions of these three SNPs between the SCOS and control groups (p>.05). This study suggests a lack of association of PAPOLB with azoospermia due to SCOS in humans. - Source: PubMed
Publication date: 2019/02/11
Miyamoto TShin TIijima MMinase GOkada HSaijo YSengoku K