Ask about this productRelated genes to: KLHL23 antibody
- Gene:
- KLHL23 NIH gene
- Name:
- kelch like family member 23
- Previous symbol:
- -
- Synonyms:
- MGC2610, FLJ37812, MGC22679
- Chromosome:
- 2q31.1
- Locus Type:
- gene with protein product
- Date approved:
- 2005-05-19
- Date modifiied:
- 2015-11-18
Related products to: KLHL23 antibody
Related articles to: KLHL23 antibody
- Perfluoroalkyl and polyfluoroalkyl substances (PFAS) are toxicants of emerging concern due to their abundance in the environment and potential adverse health effects. PFAS are used in waterproof clothing, makeup, carpets, upholstery, cookware, and fast-food containers. Due to their universal use, they are found globally in the water supply. In fact, these organic pollutants have been found in the blood of 98% of Americans and have been linked to disruption in thyroid hormone biosynthesis and availability. Moreover, several studies have shown that cancer patients may have an increase in PFAS levels and that PFAS exposure increases thyroid cancer risk. Demonstration of concrete PFAS-mediated alterations in thyroid histology and function could have far-reaching implications. To understand the effects of PFAS on thyroid histology, we used a PFAS feeding model with a combination of 3 PFAS compounds (PFOS, PFOA, and GenX) vs control. Mice receiving PFAS treatment showed altered thyroid architecture and cell structure following PFAS exposure. RNA-sequencing data revealed several alterations in gene expression and multiple signaling pathways were dysregulated. Understanding the role of PFAS-mediated toxicity in the thyroid is critically important for the prevention of thyroid disease in the general population. - Source: PubMed
Publication date: 2025/12/18
Hartmann Heather ACaroland Kailey PTumbic Goran WRampy JessicaChen Hua-ChangChen Sheau-ChiannMannes ClaraWahoski Claudia CLoberg Matthew ALiang CynthiaDiaz DianaTigue Megan LSheng QuanhuYe FeiLee EthanWeiss Vivian L - F-Actin cytoskeleton remodeling is vital for cell migration, organ development and immune responses. The small GTPase CDC42, a key regulator of F-actin dynamics, cycles between inactive GDP- and active GTP-bound states. However, mechanisms governing CDC42 turnover and their biological significance remain unclear. Here we show that KLHL23-mediated polyubiquitylation of CDC42•GTP and RhoGDI-mediated sequestration of CDC42•GDP spatiotemporally co-inactivate CDC42, preserving membrane dynamics and homeostasis during migration. KLHL23-Cul3 acts as the E3 ligase for CDC42 degradation, with KLHL23 and RhoGDI competing for CDC42's switch II region, enhancing selectivity toward CDC42•GTP and CDC42•GDP, respectively. KLHL23 depletion disrupts membrane homeostasis, inducing excessive protrusions and promoting metastasis. Notably, the CDC42-Y64C germline variant in Takenouchi-Kosaki Syndrome escapes KLHL23-mediated degradation. Fluorescence resonance energy transfer assays reveal that KLHL23 and RhoGDI coordinately inactivate CDC42 in a spatiotemporal manner. These findings highlight the biological and clinical relevance of the KLHL23/RhoGDI-CDC42 axis, presenting new avenues for therapeutic exploration. - Source: PubMed
Publication date: 2025/08/22
Liao Po-ChengChang Hao-ChunLiu Yi-ChungHung Yi-HanYang Chun-JuLin Chu-ChiLyu Ping-ChiangHsieh Sen-Yung - Age-related cancers are characterized by impaired protein homeostasis, where Kelch protein superfamily members have showed accumulating clues as critical regulators. In this paper, the cancerous role of Kelch-like family member 23 (KLHL23) was comprehensively analyzed with TCGA and single cell GEO database across overall 33 cancer types. By multi-omics analysis upon the transcriptomic, genomic, and methylation data, the current study explored the association of KLHL23 with patient survival, gene ontology, tumor-infiltrating lymphocytes, and drug responses. The correlation of copy number variations and methylation with dysregulated expression of KLHL23 were also addressed. Notably, KLHL23 levels correlated with survival in cancers such as hepatocellular carcinoma and low-grade glioma. The study also highlighted how reduced KLHL23 expression is linked to increased immune activity and sensitivity to chemotherapy, suggesting its potential as a biomarker for cancer prognosis and treatment responsiveness. - Source: PubMed
Publication date: 2024/12/04
Xu LiangliangLi BoLiu YuchenHu ZhengmingDan QingXu BingxuanXiang HongjinChen YunZheng TingtingSun DeshengLiu Li - Hepatocellular carcinoma (HCC) is one of the most common malignancies globally with poor prognosis. Cancer-associated fibroblasts (CAFs) play multiple functions in the regulation of tumorigenesis, metastasis and therapeutic resistance of cancer. The current study aimed to explore the role of CAFs-related genes in the prognosis and immunotherapy response in HCC. CAFs-related genes were identified by using single-cell RNA-sequencing analysis. Least absolute shrinkage and selection operator (LASSO) analysis was conducted to develop a CAFs-related prognostic signature (FRPS) in TCGA dataset and verified in ICGC, GSE14520 and GSE76427 cohorts. Several tools, including Tumor Immune Dysfunction and Exclusion (TIDE) score, immunophenoscore, and Tumor Mutation Burden (TMB) score were used to evaluate the value of FRPS in predicting immunotherapy benefits. The FRPS constructed based on 10 genes (RGS5, CNN3, PALLD, FLNA, KLHL23, MYC, NDRG2, SERPINE1, CD151 CALU) served as an independent risk factor and showed stable and powerful performance in predicting the overall survival rate of HCC patients with an AUCs of 0. 734, 0.727, and 0.717 in 2-, 3-, and 4-year ROC curve in TCGA cohort. Low risk score indicated a higher abundance of CD8+ T cells and NK, and lower abundance of Treg. Moreover, HCC patients with low risk score had a higher PD1&CTLA4 immunophenoscore, higher TMB score, and lower TIDE score. Moreover, high risk score indicated a lower IC50 value of 5-fluorouracil, camptothecin, cisplatin, docetaxel, gemcitabine, paclitaxel, afatinib, crizotinib, dasatinib, erlotinib, erlotinib, gefitinib, lapatinib, and osimertinib in HCC. Our study develops a novel FRPS HCC. The FRPS acts as a risk factor for the prognosis of HCC patients and it can predict the immunotherapy benefits of HCC patients. - Source: PubMed
Ye JianzhongTian WenZheng BigengZeng Tao - Direct-acting antivirals (DAAs) have achieved a sustained virological response (SVR) rate of 95-99% in treating HCV. Several studies suggested that treatment with sofosbuvir (SOF), one type of DAAs, may be associated with increased risk of developing HCC. The aim of this study is to investigate the potential mechanisms of SOF on the development of HCC. OR-6 (harboring full-length genotype 1b HCV) and Huh 7.5.1 cells were used to examine the effects of SOF on cell proliferation and migration of HCC cells. SOF-upregulated genes in OR-6 cells were inspected using next generation sequencing (NGS)and the clinical significance of these candidate genes was analyzed using The Cancer Genome Atlas (TCGA) database. We found that SOF increased cell proliferation and cell migration in OR-6 and Huh 7.5.1 cells. Several SOF-upregulated genes screened from NGS were confirmed by real-time PCR in OR-6 cells. Among these genes, PHOSPHO2, KLHL23, TRIM39, TSNAX-DISC1 and RPP21 expression were significantly elevated in the tumor tissues compared with the non-tumor tissues of HCC according to TCGA database. High expression of PHOSPHO2 and RPP21 was associated with poor overall survival of HCC patients. Moreover, knockdown of PHOSPHO2-KLHL23, TSNAX-DISC1, TRIM39 and RPP21 diminished cell proliferation and migration increased by SOF in OR-6 and Huh 7.5.1 cells. In conclusion, SOF-upregulated genes promoted HCC cell proliferation and migration, which might be associated with the development of HCC. - Source: PubMed
Publication date: 2022/07/12
Tsai Wei-LunCheng Jin-ShiungLiu Pei-FengChang Tsung-HsienSun Wei-ChihChen Wen-ChiShu Chih-Wen