Ask about this productRelated genes to: PCBP3 antibody
- Gene:
- PCBP3 NIH gene
- Name:
- poly(rC) binding protein 3
- Previous symbol:
- PCBP3-OT1, PCBP3OT
- Synonyms:
- FLJ44028
- Chromosome:
- 21q22.3
- Locus Type:
- gene with protein product
- Date approved:
- 2000-05-23
- Date modifiied:
- 2017-01-19
Related products to: PCBP3 antibody
Related articles to: PCBP3 antibody
- Epithelial cells exhibit a highly polarized organization along their apico-basal axis, a feature that is critical to their function and is frequently perturbed in cancer. One less explored process modulating epithelial cell polarity is the subcellular localization of mRNA molecules. In this study, we report that several mRNAs encoding evolutionarily conserved epithelial polarity regulatory proteins, including , , and , are localized to cell junction regions in epithelial tissues and human epithelial cells. Targeting of these mRNAs coincides with robust junctional distribution of their encoded proteins, and these transcripts are translated in proximity to cell junction regions. Through systematic immunolabeling, we identify a collection of RNA binding proteins with cell junction distribution patterns, several of which associate with junctional transcripts and are functionally required for proper targeting of ZO-1 and SCRIB proteins. Loss of function of two candidate factors, MAGOH and PCBP3, differentially impacts junctional mRNA, with MAGOH knockdown reducing and transcript targeting and localized translation, while PCBP3 knockdown only perturbs local translation. Depletion of MAGO in vivo in follicular epithelial cells also disrupts the distribution of junctional transcripts and proteins. Finally, through tissue microarray analysis of ovarian cancer tumor specimens, we find that the expression of MAGOH and ZO-1 is positively correlated and that both proteins are potential biomarkers of good prognosis. We conclude that localized mRNA regulation at cell junction regions is important for modulating epithelial cell integrity. - Source: PubMed
Publication date: 2026/04/16
Chin AshleyBergeman JonathanCommunal LaudineBarutcu SedaBoulais JonathanYeo Gene WMes-Masson Anne-MarieLécuyer Eric - Cervical cancer ranks among the most prevalent malignancies impacting women globally. Disulfidptosis represents a recently identified pathway of cellular demise, although its role in the context of cervical cancer is not well elucidated. This research investigates the significance of Disulfidptosis-Related Genes (DRGs) within cervical cancer. Furthermore, it aims to analyze the differences in prognosis and immune infiltration among different molecular subtypes. - Source: PubMed
Publication date: 2025/05/14
Yao YadanYang XiaominFu YuanxinZhang Yinmin - Climate emergency and ecological sustainability call for new ways of thinking livestock health, including the dairy cattle. This study unpacks the genetic diversity and selection sweeps of Sahiwal cattle in relation to adaptability, production, and disease resistance. Using nucleotide diversity (π) calculated from 10 kb windows across the genome with VCFtools, 716 regions of genetic diversity were identified across 29 chromosomes, and importantly, with chromosome 15 showing the highest density. A total of 92 quantitative trait loci (QTL) linked genes were analyzed, with chromosome 1 harboring the highest number. Trait association analysis using the Cattle QTL database showed that 14 genes were linked to production traits, 10 to reproduction traits, and 8 to disease susceptibility. Notable genes included and , which influence milk production traits such as fat percentage and yield, and and , which affect reproductive traits. Additionally, the genes and were associated with disease traits such as bovine respiratory disease and sole ulcers. Selection sweeps, identified using Tajima's D, revealed 728 sweeps across the genome, with chromosomes 6 and 8 showing the highest frequencies. These sweeps indicate regions under strong selective pressure, likely due to the breed's adaptation to arid environments and specific trait selection. The present study highlights how genetic diversity and selection sweeps contribute to Sahiwal cattle's adaptability, production efficiency, and disease resistance. The insights reported here provide a foundation for livestock health and targeted breeding strategies in the case of Sahiwal cattle under diverse ecological conditions such as tropical climate. - Source: PubMed
Publication date: 2025/04/21
Chaudhary PradeepMagotra AnkitAlex RaniBangar Yogesh CSindhu PushpaRose Manoj KGarg Asha R - Kaposi's sarcoma (KS) is a locally aggressive, multicentric tumor. RNA-binding proteins (RBPs) are pivotal for post-transcriptional regulation in various tumors. However, the aberrantly expressed RBP genes and their regulatory patterns in KS remain unclear. This study aimed to identify relevant RBP genes in KS and assess the potential functions and molecular interactions of RPS27, a dysregulated RBP in KS tissues, METHODS: Matched KS lesions and normal control tissues from ten patients were chosen for the study. Differentially expressed genes (DEGs) were first identified by RNA-sequencing, and results were validated through an independent public RNA-seq dataset (GSE147704). Among the DEGs, RBPs were selected for further analysis, with RPS27 chosen for detailed investigation due to its dysregulation in KS tissues. RT-qPCR and immunohistochemistry were employed to validate RPS27 expression. Cellular experiments were conducted for dysregulated RPS27 to explore its functions. Additionally, improved RNA immunoprecipitation (iRIP)-seq was performed to investigate potential binding interactions of RPS27 in KS. - Source: PubMed
Publication date: 2025/02/27
Zhang JingzhanWang PengLi TingtingLuo DongQu YuanyuanDing YuanKang Xiaojing - Exploring the mechanism of breast cancer metastasis and searching for new drug therapeutic targets are still the focuses of current research. RNA-binding proteins (RBPs) may affect breast cancer metastasis by regulating alternative splicing (AS) during epithelial-mesenchymal transition (EMT). We hypothesised that during EMT development in breast cancer cells, the expression level of RBPs and the gene AS pattern in the cell were significantly changed on a genome-wide scale. Using GEO database, this study identified differentially expressed RBPs and differential AS events at different stages of EMT in breast cancer cells. By establishing the correlation network of differential RBPs and differential AS events, we found that RBM47, PCBP3, FRG1, SRP72, RBMS3 and other RBPs may regulate the AS of ITGA6, ADGRE5, TNC, COL6A3 and other cell adhesion genes. By further analysing above EMT-related RBPs and AS in breast cancer tissues in TCGA, it was found that the expression levels of ADAT2, C2orf15, SRP72, PAICS, RBMS3, APOBEC3G, NOA1, ACO1 and the AS of TNC and COL6A3 were significantly correlated with the prognosis of breast cancer patients. The expression levels of all 8 RBPs were significantly different in breast cancer tissues without metastasis compared with normal breast tissues. Conclusively, eight RBPs such as RBMS3 and AS of TNC and COL6A3 could be used as predictors of breast cancer prognosis. These findings need to be further explored as possible targets for breast cancer treatment. - Source: PubMed
Publication date: 2024/05/23
Mi YinDong MeilianZuo XiaoxiaoCao QinchenGu XiaobinMi HailongXiao Fankai