Ask about this productRelated genes to: RTCD1 antibody
- Gene:
- RTCA NIH gene
- Name:
- RNA 3'-terminal phosphate cyclase
- Previous symbol:
- RTCD1
- Synonyms:
- RPC, RTC1
- Chromosome:
- 1p21.2
- Locus Type:
- gene with protein product
- Date approved:
- 2002-01-10
- Date modifiied:
- 2016-10-05
Related products to: RTCD1 antibody
Related articles to: RTCD1 antibody
- Coffee extracts contain numerous bioactive compounds. Given the dietary link between coffee consumption and colorectal cancer, this study compared the effects of roasted and green (unroasted) coffee extracts on human colorectal cancer cells (HCT116) and non-cancerous fibroblasts (BJ-5ta) to evaluate how processing influences proliferation and molecular signaling. Real-time cell analysis (RTCA), qRT-PCR, and label-free quantitative proteomic analysis were performed to assess cellular responses. MTS and RTCA showed that in BJ-5Ta fibroblasts, coffee extracts increased proliferation in the order CNR < CAR < CAU < CNU, whereas the trend was reversed in HCT116 cancer cells. Proteomic analysis revealed that in BJ-5Ta cells, unroasted coffee extract caused downregulation of the ribosome pathway, and natural coffee extract caused downregulation of the gap junction pathway, indicating reduced protein synthesis and cell-cell communication as a potential stress-adaptive response. In contrast, in HCT116 cells, unroasted coffee extract upregulated the ribosome pathway. Also, natural coffee extract upregulated the pentose phosphate pathway in HCT116 cells, which may enhance NADPH production and reduce oxidative stress. Current evidence suggests coffee's bioactive compounds may have different effects varying by coffee extract type and their preparation. - Source: PubMed
Šemeláková MBober PVárady MHudáková THarvanik PŠalagovič JPopelka PSolár P - Resin-based restorative materials (RBRMs) are extensively used in contemporary dentistry; however, their biocompatibility remains a concern due to the release of residual monomers. Finishing-polishing procedures may significantly influence surface characteristics and subsequent cytotoxic behavior, yet dynamic evidence using the real-time cell analysis (RTCA) is limited. This research was done to evaluate the outcome of different finishing-polishing procedures on the cytotoxicity of RBRMs using RTCA. - Source: PubMed
Publication date: 2026/04/24
Ismail Prabu Mahin Syed - Plastispheres host unique microbial communities linked to biogeochemical cycling and ecological risks, yet their responses to pollutant enrichment and seasonal dynamics in the Danjiangkou (DJK) Reservoir, China's core South-to-North Water Diversion water source, are poorly understood. We sampled plastispheres and ambient water at seven DJK tributary estuaries (August 2024/wet, January 2025/dry), characterizing pollutant enrichment, microbial assembly (16S rRNA sequencing), functional gene expression (qPCR), and harmful microbial enrichment, with quantitative microbial risk assessment (QMRA). Results demonstrated that the plastisphere serves as a critical sink for aquatic pollutants, leachate from the plastisphere contains 181.20 ± 27.35%, 875.98 ± 77.61%, and 286.21 ± 32.47% higher concentrations of total nitrogen (TN), ammonium (NH₄), and lead, respectively, than ambient water, and no significant seasonal variation in plastic adsorption capacity. Plastisphere microbes had higher alpha diversity (Chao1: +172.41%; Shannon: +28.07%) and network stability, with Proteobacteria (e.g., Pseudomonas) enriched in summer and Flavobacteria dominating winter plastispheres. Functional genes linked to nitrogen cycling (nitrogen fixation: nifH; denitrification: nirS, nirK) and carbon metabolism (rTCA cycle: aclA, pflD; methane formation: mtaABC, mtbA, cdhCDE) were significantly upregulated in the plastisphere, with total RPKM values 30.49 ± 18.64% higher than in ambient water (p<0.05), with water temperature and DO as primary drivers. Notably, plastics selectively enriched harmful microbes, cyanobacterial taxon SIO2C1_sp010672925 (18.80 ± 5.14%) dominated winter plastispheres, and 524 pathogenic OTUs (e.g., Deinococcus_B, 6.70 ± 3.88%) were more abundant on plastispheres, posing non-negligible risks of algal blooms and pathogen dissemination. Our findings reveal that plastics mediate pollutant accumulation, microbial assembly, and functional gene enrichment in tributary estuaries, with seasonal dynamics shaping harmful bacteria proliferation. This study provides critical insights into the ecological impacts driven by plastic pollution in key freshwater reservoirs, supporting targeted water quality management and ecological risk mitigation. - Source: PubMed
Publication date: 2026/04/20
He TaoXu Zhi-MinCheng Le-YiZhang Yi-CaiWang ShuangZhang Xiao-YanYang XiHou Yu-TingWang Si-YiHu Xin-YueZhou Xin-RuiMa Yong-HongZhou Huan-Zhan - : Toothpaste ingredients such as strontium chloride (SrCl) and potassium carbonate (KCO) are recognized for their desensitizing and remineralizing effects but may be absorbed through the oral mucosa. Their potential cytotoxic and cardiotoxic properties, however, remain inadequately characterized. Here, we investigated the effects of SrCl and KCO on mouse-induced pluripotent stem cells (iPSCs) and iPSC-derived cardiomyocytes (iPSC-CMs). Cells were exposed to varying concentrations of each compound for up to 72 h. Real-time cell analysis (xCELLigence RTCA Cardio system) was used to assess proliferation, and flow cytometry was used to evaluate cell viability. Functional properties of iPSC-CMs were examined using multi-electrode array (MEA) recordings and xCELLigence-based impedance measurements. Cardiac marker expression was examined via immunofluorescence and quantitative RT-PCR. Both SrCl and KCO affected iPSC proliferation and reduced viability in a dose- and time-dependent manner, accompanied by altered embryoid body (EB) morphology and increased cell death. In iPSC-CMs, both compounds downregulated key cardiac genes and disrupted spontaneous beating activity, with effects intensifying at higher concentrations. These results demonstrate that SrCl and KCO induced dose-dependent cytotoxic and arrhythmogenic effects on iPSCs and iPSC-CMs. At elevated concentrations, these compounds impair iPSC-CM function and may pose safety concerns upon chronic exposure. Further mechanistic and long-term in vivo studies are warranted to assess their potential cardiotoxic risk in consumer oral care products. - Source: PubMed
Publication date: 2026/02/25
Kumar SaheeraKamga Kapchoup Michelle VanessaZhang HaiPerumal Srinivasan SureshkumarKaptue Wuyt AdelineTsafack Zefack JudeHescheler JürgenNguemo Filomain - Water disinfection unintentionally produces hundreds of disinfection byproducts (DBPs). Although the cytotoxicity of over 100 DBPs has been extensively studied in Chinese hamster ovary (CHO) cells, toxicity data in human cell models remain limited. Given the epidemiological association of DBPs with bladder cancer risk, it is necessary to conduct studies using cell lines that exhibit biological characteristics resembling human bladder cells, e.g., the immortalized human uroepithelium SV-HUC-1 cells. In this study, the cytotoxicity of 14 regulated and unregulated DBPs in SV-HUC-1 and CHO-K1 cells was examined in parallel using a real-time cell analysis (RTCA) platform. SV-HUC-1 cells exhibited greater sensitivity to eight DBPs than CHO-K1 cells. Notably, iodoacetic acid (IAA) and bromoacetic acid (BAA) were significantly more cytotoxic to SV-HUC-1 cells than iodoacetonitrile (IAN) and bromoacetonitrile (BAN), whereas the reverse trend was observed in CHO-K1 cells. Cell cycle analysis showed that only IAN and BAN induced G2/M phase arrest in SV-HUC-1 cells. In contrast, in CHO-K1 cells, IAN caused G2/M arrest, BAN and CAN induced G0/G1 arrest, and IAA and BAA arrested cells in the S phase. Morphological assessments showed that SV-HUC-1 cells aggregated upon exposure to monohaloacetic acids (monoHAAs), while exposure to monohaloacetonitriles (monoHANs) led to cell shrinkage and nuclear lysis. CHO-K1 cells maintained a spindle-shaped morphology with reduced size under monoHAA exposure, whereas monoHAN treatment induced an elliptical shape, with a notable subset transitioning to hypertrophic hyperploidy. These findings highlight cell- and DBP-specific toxic effects, stressing the need to include human cell lines in future DBP toxicity assessments. - Source: PubMed
Publication date: 2025/05/09
Xie JiaojiaoHan JianLiu YanmingMoe BirgetShen QimingXiang TongtongYuan Chun-GangLi Xing-Fang