Ask about this productRelated genes to: PAPOLG antibody
- Gene:
- PAPOLG NIH gene
- Name:
- poly(A) polymerase gamma
- Previous symbol:
- -
- Synonyms:
- FLJ12972
- Chromosome:
- 2p16.1
- Locus Type:
- gene with protein product
- Date approved:
- 2001-05-22
- Date modifiied:
- 2014-11-19
Related products to: PAPOLG antibody
Related articles to: PAPOLG antibody
- One of the unknowns related to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is the mechanism underlying the inflammatory response induced by the virus. Poly(A) polymerase gamma (PAPOLG) was previously shown to be upregulated during SARS-CoV-2 infection. The present study explored how PAPOLG affects the inflammatory reaction triggered by SARS-CoV-2. PAPOLG was knocked down or overexpressed in THP-1 macrophages. Target pathways were identified using RNA sequencing and bioinformatics analysis. The levels of PAPOLG, transcriptional regulator nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), and cytokines TNF-α and IL-6 were measured, along with an assessment of NF-κB mRNA stability. PAPOLG was significantly upregulated in SARS-CoV-2-infected THP-1 macrophages. Genes subjected to alternative polyadenylation were enriched in immune pathways, and NF-κB emerged as a key regulator. Knockdown of PAPOLG promoted NF-κB mRNA degradation, while decreasing the levels of tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL6). Conversely, overexpression of PAPOLG stabilized NF-κB mRNA and enhanced TNF-α and IL-6 expression. PAPOLG contributes to the inflammatory response in SARS-CoV-2-infected macrophages by stabilizing NF-κB mRNA. Thus, PAPOLG may be targeted to control COVID-19-related inflammation. - Source: PubMed
Liao YanyanChen QiuliWang HailongPan PeijiangZhou LijuanJiang JunjunLan QiyuanXu XinyueLin XuePeng YuanjunYang YaqianAn SanqiYe LiLiang Hao - Premature transcription termination yields a wealth of unadenylated (pA) RNA. Although this can be targeted for degradation by the Nuclear EXosome Targeting (NEXT) complex, possible backup pathways remain poorly understood. Here, we find increased levels of 3' end uridylated and adenylated RNAs upon NEXT inactivation. U-tailed RNAs are mostly short and modified by the cytoplasmic tailing enzymes, TUT4/7, following their PHAX-dependent nuclear export and prior to their degradation by the cytoplasmic exosome or the exoribonuclease DIS3L2. Longer RNAs are instead adenylated redundantly by enzymes TENT2, PAPOLA and PAPOLG. These transcripts are either degraded via the nuclear Poly(A) tail eXosome Targeting (PAXT) connection or exported and removed by the cytoplasmic exosome in a translation-dependent manner. Failure to do so decreases global translation and induces cell death. We conclude that post-transcriptional 3' end modification and removal of excess pA RNA is achieved by tailing enzymes and export factors shared with productive RNA pathways. - Source: PubMed
Publication date: 2024/12/01
Wu GuifenRouvière Jérôme OSchmid ManfredHeick Jensen Torben - 2p15p16.1 microdeletion syndrome was described for the first time in 2007. The size of the microdeletion is variable and encompasses several genes, like , and . Features of the syndrome include short stature, microcephaly, hypotonia, psychomotor developmental delay, anomalies of the fingers of the upper and lower limbs, dysmorphic features like receding forehead, broad nasal bridge, telecanthus, ptosis, flat philtrum, small mouth with a high, narrow palate and everted lower lip. The precise genotype-phenotype correlation in 2p15 deletion syndrome is not understood. The aim of the study is to present the patient's medical history and the diagnostic process. - Source: PubMed
Publication date: 2024/07/15
Ręka GabrielaWojciechowska KatarzynaLejman Monika - Mastitis is one of the most common diseases of dairy cattle and can be caused by physical stress, chemicals and microbial infection. Staphylococcus aureus is the most common pathogens that induce mastitis in dairy cattle. In this study, bovine mammary epithelial cells (BMECs) were treated either with lipoteichoic acid (LTA, 30 µg/ml) or 1 × phosphate-buffer saline (PBS, control) and RNA-Seq was applied to explore the effect of LTA on the expression microRNAs (miRNAs) in BMECs. Compared to the control group, 43 miRNAs were significantly up-regulated and eight miRNAs were significantly down-regulated. Additionally, 724 genes were significantly up-regulated and 13 genes were significantly down-regulated in LTA group relative to the control group. Bta-miR-196a, bta-miR-2285aj-5p, bta-miR-143, bta-miR-2433, bta-miR-2284f and bta-miR-2368-3p were selected from 51 differentially expressed miRNAs and are discussed in this manuscript. Target gene prediction revealed that the target genes of these six miRNAs were all differentially expressed, including MT1E, SPDYA, FGL1, TLR2, PAPOLG, ZDHHC17 and SMC4. Subsequently, the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that the target genes with differentially expressed miRNAs were enriched in mitogen-activated protein kinase (MAPK) signalling pathway, rheumatoid arthritis and cancer. Therefore, the results of this study provided new evidences for the molecular mechanism of LTA-induced mastitis, which may provide new targets for the diagnosis and treatment of mastitis in dairy cattle. - Source: PubMed
Publication date: 2022/08/23
Shen PuxiuYu JingchengYan ChenboYang DexinTong ChaoWang Xinzhuang - Superovulation treatments aim to stimulate multifollicular recruitment, maximizing the number of oocytes or transferable embryos produced. Factors associated with the superovulation protocol, female characteristics and many other factors are determinants in the number and quality of oocytes obtained. An accurate way to assess oocyte quality more precise than morphological appearance is genetic expression. The present study aims to compare the response of nulliparous and multiparous females to superovulatory stimulation, studying its effect on the expression of some genes associated with the activation, growth, development and oocyte-embryo transition of oocytes, as well as its impact on in vivo embryonic development and viability rate at birth. In a first experiment, the effect of stimulation treatment on the ovulation response and the expression of the MSY2, MATER, ITPR1, ITPR2, ITPR3, eIF4E, PAR1, PAPOL-A, PAPOL-G, ZAR1 and YY1 genes in nulliparous and multiparous females were determined. In a second experiment, the implantation and viability at birth of embryos from superovulated nulliparous and multiparous females were analysed. The ovulation rate was significantly higher in the superovulation groups than in the control groups. The ovulation rate was significantly increased in nulliparous females compared with multiparous does. From the eleven genes analysed, only the expression of MATER, PAPOL-A, PAPOL-G and ZAR-1 genes was shown to be different among experimental groups. Finally, in terms of implantation rate and viability at birth, the nulliparous control group showed better results than the rest of the groups. Both hyperstimulation treatment and reproductive female's history seem to alter the transcriptome of important genes related to oocyte maturation and competence acquisition, affecting in vivo embryo viability. - Source: PubMed
Publication date: 2022/06/10
Vicente J SMarco-Jiménez FPérez-García MNaturil-Alfonso CPeñaranda D SViudes-de-Castro M P