Ask about this productRelated genes to: PXT1 antibody
- Gene:
- PXT1 NIH gene
- Name:
- peroxisomal testis enriched protein 1
- Previous symbol:
- -
- Synonyms:
- STEPP
- Chromosome:
- 6p21.31
- Locus Type:
- gene with protein product
- Date approved:
- 2004-04-30
- Date modifiied:
- 2018-02-22
Related products to: PXT1 antibody
Related articles to: PXT1 antibody
- The long-term immunological effects of prior Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection in people living with Human Immunodeficiency Virus (HIV) remain poorly understood. This study aimed to characterize plasma proteomic alterations associated with previous SARS-CoV-2 infection in HIV-infected individuals and to identify potential biomarkers and affected pathways. High-throughput liquid chromatography–tandem mass spectrometry (LC–MS/MS) was performed on plasma obtained from three groups: HIV-infected individuals with documented prior SARS-CoV-2 infection, HIV-monoinfected individuals, and healthy controls. A total of 13,675 proteins were identified. Hierarchical clustering and sparse partial least squares discriminant analysis revealed distinct proteomic profiles in the prior-SARS-CoV-2 group. Ten proteins with the highest discriminatory power—PRR11, TEX14, METTL9, NMD3, PXT1, CRISP2, MELK, SPF27, GCP6, and GTPBP8—were associated with cell cycle regulation, RNA processing, apoptosis, mitochondrial function, and cytoskeletal organization. Subcellular localization indicated predominant nuclear and cytoplasmic involvement, suggesting alterations in transcriptional regulation and intracellular structural dynamics. These signatures imply that preceding SARS-CoV-2 exposure may compound HIV-associated immune dysregulation and disrupt cellular homeostasis. The findings offer novel molecular insights into the persistent biological impact of SARS-CoV-2 in the context of HIV and identify candidate proteomic biomarkers with potential utility for risk stratification and targeted intervention in immunocompromised populations. - Source: PubMed
Publication date: 2025/12/09
Chanthara ChayaninKhattiya JanyaRoytrakul SittirukAkekawatchai ChareepornPhaonakrop NarumonNiyomdecha Nattamon - Acute lymphoblastic leukemia (ALL), the most common type of pediatric leukemia, is frequently driven by fusion genes generated by chromosomal rearrangements. Compared with wild-type genes, many oncogenic fusions show increased expression and sustained functional activity that drives tumorigenesis. However, the mechanisms by which chromosomal rearrangements lead to functional enhancement remain largely elusive. In addition, although large-scale sequencing has identified numerous fusion events, the functional significance of most remains unclear. Here, we demonstrate that enhanced mRNA stability represents an important tumorigenic mechanism for oncogenic fusions, including classical PAX5 fusions. Based on this mechanism, we characterize a novel oncogenic fusion, STK38-PXT1, which exhibits upregulated STK38 mRNA levels and drives the development of ALL. Mechanistically, the increased mRNA stability results primarily from enhanced N6-methyladenosine modification of oncogenic fusions, which is attributable to "gene truncation" (as in PAX5 fusions) and "partner collaboration" (as in STK38-PXT1). Furthermore, the m6A reader IGF2BP3 is crucial for maintaining the high mRNA stability of oncogenic fusions. We further propose venetoclax as an innovative and clinically available therapy for ALL driven by these oncogenic fusions characterized by high mRNA stability. Our study not only highlights mRNA stabilization as a crucial mechanism by which oncogenic fusions drive tumorigenesis, but also presents a promising therapeutic strategy for patients with ALL. - Source: PubMed
Publication date: 2025/11/13
Shao XuejingXia ZhimeiCai MinyiShao ChenBing ShaoweiWang TianruiDu WenxinLiu JiayiShen DiyingCao JiYang BoHe QiaojunXu XiaojunZhang JingyingYing Meidan - Mouse Pxt1 gene is expressed exclusively in male germ cells and encodes for a small, cell death inducing protein. However, upon PXT1 interaction with BAG6, cell death is prevented. In transiently transfected cell lines the PXT1 expression triggered massive cell death, thus we ask the question whether the interaction of PXT1 and BAG6 is the only mechanism preventing normal, developing male germ cells from being killed by PXT1. The Pxt1 gene contains a long 3'UTR thus we have hypothesized that Pxt1 can be regulated by miRNA. We have applied Pxt1 knockout and used Pxt1 transgenic mice that overexpressed this gene to shed more light on Pxt1 regulation. Using the ELISA assay we have demonstrated that PXT1 protein is expressed in adult mouse testis, though at low abundance. The application of dual-Glo luciferase assay and the 3'UTR cloned into p-MIR-Glo plasmid showed that Pxt1 is regulated by miRNA. Combining the use of mirDB and the site-directed mutagenesis further demonstrated that Pxt1 translation is suppressed by Mir6996-3p. Considering previous reports and our current results we propose a model for Pxt1 regulation in the mouse male germ cells. - Source: PubMed
Publication date: 2023/07/11
Tomczyk IgorRokicki MikołajSieńko WioletaRożek KatarzynaNalepa AnnaWiench JasminGrzmil Paweł - The Pxt1 gene encodes a male germ cell-specific protein and its overexpression results in male germ cell degeneration and male infertility in transgenic mice. - Source: PubMed
Pawlicka BernadettaDuliban MichalZieba MateuszBochenek MichalZieba KamilaAdham IbrahimStudencka-Turski MajaMeinhardt AndreasGrzmil Pawel - Bak is a critical executor of apoptosis belonging to the Bcl-2 protein family. Bak contains a hydrophobic groove where the BH3 domain of proapoptotic Bcl-2 family members can be accommodated, which initiates its activation. Once activated, Bak undergoes a conformational change to oligomerize, which leads to mitochondrial destabilization and the release of cytochrome c into the cytosol and eventual apoptotic cell death. In this study, we investigated the molecular aspects and functional consequences of the interaction between Bak and peroxisomal testis-specific 1 (Pxt1), a noncanonical BH3-only protein exclusively expressed in the testis. Together with various biochemical approaches, this interaction was verified and analyzed at the atomic level by determining the crystal structure of the Bak-Pxt1 BH3 complex. In-depth biochemical and cellular analyses demonstrated that Pxt1 functions as a Bak-activating proapoptotic factor, and its BH3 domain, which mediates direct intermolecular interaction with Bak, plays a critical role in triggering apoptosis. Therefore, this study provides a molecular basis for the Pxt1-mediated novel pathway for the activation of apoptosis and expands our understanding of the cell death signaling coordinated by diverse BH3 domain-containing proteins. - Source: PubMed
Publication date: 2023/06/14
Lim DahwanChoe So-HuiJin SeinLee SeulgiKim YounjinShin Ho-ChulChoi Joon SigOh Doo-ByoungKim Seung JunSeo JinhoKu Bonsu