Ask about this productRelated genes to: PDXK antibody
- Gene:
- PDXK NIH gene
- Name:
- pyridoxal kinase
- Previous symbol:
- C21orf97, C21orf124
- Synonyms:
- PNK, PKH, FLJ21324, PRED79, FLJ31940, MGC15873
- Chromosome:
- 21q22.3
- Locus Type:
- gene with protein product
- Date approved:
- 1998-12-03
- Date modifiied:
- 2017-01-11
Related products to: PDXK antibody
Related articles to: PDXK antibody
- Pyridoxal 5'-phosphate (PLP) is an essential cofactor required for metabolic functions including amino acid biosynthesis. While the pathways of PLP synthesis are well characterized, it is unclear how newly synthesized PLP is delivered to apo PLP-dependent enzymes (PLP-DEs) in the cell. The highly conserved PLP Homeostasis Protein (PLPHP) is hypothesized to act as a PLP carrier that traffics the cofactor to PLP-DEs. Prior studies have shown transfer of PLP between PLPHP homologs and PLP-DEs in vitro. Data herein support a role for this transfer in vivo. This study utilizes a Salmonella enterica mutant strain with a synthetic requirement for PLPHP homolog, YggS. Genetic and biochemical analyses suggest that the synthetic phenotype of a yggS aspC mutant results from inefficient maturation of the aromatic amino acid aminotransferase, TyrB. This requirement for YggS can be bypassed with accumulation of B vitamer PMP, or upregulation of pyridoxal kinase (PdxK). Using TyrB as a target, we provide the first demonstration that YggS of S. enterica can mediate transfer of PLP to a PLP-DE in vitro. In total, this study defines multiple in vivo maturation pathways for TyrB and provides a working model for the role of YggS in the maturation of TyrB. - Source: PubMed
Publication date: 2026/04/27
Buckner Brandi ADowns Diana M - Pyridoxal 5'-phosphate (PLP), a vital cofactor in cellular metabolism, is a high-value compound widely used in the food and pharmaceutical industries. However, the efficient biosynthesis of PLP remains a significant challenge. In this study, we established a salvage pathway for the synthesis of PLP from pyridoxine (PN) using PN kinase (PdxK) from and pyridoxine 5'-phosphate oxidase (PdxH) from as the key enzymes. To increase economic viability, an ATP regeneration system was integrated, enabling the use of AMP and polyP as substrates instead of expensive ATP. Additionally, an O circulating system was incorporated to provide sufficient oxygen supply and a HO-free environment. PdxH was identified as the rate-limiting enzyme, so we performed directed evolution of ScPdxH through random mutagenesis coupled with high-throughput screening. The optimized mutant, ScPdxH, exhibited a 3.4-fold increase in compared to the wild-type enzyme. Using a two-step bioconversion process, we achieved the production of 35.2 g/L pyridoxine 5'-phosphate (PNP) with a molar conversion of 94.7%, and 21.3 g/L PLP, corresponding to a final molar conversion yield of approximately 57.5% from PN in a 1-L bioreactor. This work demonstrates an efficient, sustainable and scalable strategy for the industrial production of PLP. - Source: PubMed
Publication date: 2026/04/06
Yang XinweiLu BeisiWang MeilingChen YuchenLu XiyueTao YongHuang JianzhongKe Chongrong - Vitamin B6 is an essential micronutrient whose biologically active form, pyridoxal 5'-phosphate (PLP), acts as a cofactor in metabolic reactions linked to tumorigenesis and also functions as an antioxidant. Low plasma PLP levels are consistently associated with cancer, but studies on dietary intake have yielded conflicting results. Overall, evidence suggests that the effects of vitamin B6 deficiency on cancer are context-dependent, varying with cell type and tumor stage. Accordingly, high expression of and , two key genes involved in PLP biosynthesis, is associated with tumor progression in some malignancies, whereas it correlates with improved outcomes in others. This review explores as a useful model to investigate underlying mechanisms, bypassing the limitations of human studies. Research in demonstrates that PLP deficiency promotes cancer by triggering genomic instability. Furthermore, a critical PLP- gene-nutrient interaction impacting oncogenesis has been established in flies, offering significant therapeutic implications. Finally, studies in have shown that PLP deficiency can promote tumor development by also triggering the loss of heterozygosity (LOH). These findings highlight as a powerful tool to elucidate the molecular pathways linking vitamin B6 deficiency to cancer. - Source: PubMed
Publication date: 2026/03/22
Vernì FiammettaAngioli ChiaraFerriero AngeloAgostini Beatrice - PDXK-related neuropathy is a rare form of hereditary motor and sensory neuropathy with optic atrophy, caused by biallelic variants in the PDXK gene. To date, only four missense variants have been reported in 13 patients from six families. Unlike most inherited neuropathies, which currently have only symptomatic treatments, this type of neuropathy can be managed with pyridoxal 5'-phosphate supplementation. - Source: PubMed
Subbotin DmitriiAkimova DariaDadali ElenaMikhalchuk KristinaBorovikov ArtemKadyshev VitalySkoblov MikhailMurtazina Aysylu - Sodium overload-induced cell death is a novel mode of cell death that differs from other modes and is a very promising new target for cancer therapy. In addition, sodium overload-induced cell death plays a crucial role in cancer progression and patient prognosis. Therefore, our study aims to establish a survival prediction model for patients with lung adenocarcinoma (LUAD) based on related genes, exploring the immune landscape and providing new insights for future individualized treatment protocols. - Source: PubMed
Publication date: 2026/03/17
Yu Wei-JieMiao Zhou-LinAiniwaer Julaiti