Ask about this productRelated genes to: IDI1 antibody
- Gene:
- IDI1 NIH gene
- Name:
- isopentenyl-diphosphate delta isomerase 1
- Previous symbol:
- -
- Synonyms:
- -
- Chromosome:
- 10p15.3
- Locus Type:
- gene with protein product
- Date approved:
- 1995-11-08
- Date modifiied:
- 2015-08-25
Related products to: IDI1 antibody
Related articles to: IDI1 antibody
- Rapid advancements in biotechnology have enabled biomanufacturing to emerge as a feasible approach for industrial chemical production. By harnessing synthetic biology and metabolic engineering, engineered microbial cell factories can convert renewable resources into valuable chemicals, providing a sustainable alternative to traditional chemical methods. This study focuses on the microbial production of retinal, an important retinoid used in pharmaceuticals, food, and cosmetics. The oleaginous yeast NP11 was genetically modified to synthesize retinal by incorporating and optimizing three β-carotene 15,15'-dioxygenase genes from various sources. Several genetic modifications were made to enhance retinal yield, including the overexpression of isopentenyl-diphosphate isomerase (IDI1), geranylgeranyl diphosphate synthase (BTS1), phytoene synthase (CARRP), and phytoene dehydrogenase (CARB), which led to increased β-carotene levels and boosted retinal production. Furthermore, fermentation conditions such as temperature, antioxidants, and extractants were fine-tuned. The engineered strain Rt13 ultimately achieved a maximum retinal concentration of 20.38 mg/L through fed-batch fermentation. This study highlights the potential of . as a cell factory for terpenoid biosynthesis, providing valuable insights for future metabolic engineering endeavors. - Source: PubMed
Publication date: 2026/04/02
Qiu HuihuiTian LinyueHu LinWu LianwuHuang YuGe RanXing YufanKamnev Alexander AHe NingCao Mingfeng - Spinal cord injury (SCI) refers to trauma to the spinal cord resulting in functional deficits. Dysregulation of the phosphoinositide 3-kinase/serine-threonine kinase (PI3K/AKT) pathway significantly contributes to the pathogenesis of SCI. This study evaluated the role of PI3K/AKT-associated biomarkers in SCI. Transcriptomic data from SCI samples deposited in the Gene Expression Omnibus (GEO) database were analyzed. An integrative approach combining differential expression profiling, machine learning, and experimental validation was employed to identify PI3K/AKT-related biomarkers. Combinatorial strategies-including functional enrichment analysis, immune microenvironment characterization, in silico drug prediction, and ligand-receptor docking-were used to elucidate potential biomarker-driven pathological mechanisms. Quantitative reverse transcription PCR (RT-qPCR) was performed to validate biomarker expression. Four biomarkers-FGF2, IL-6, PIK3R5, and TLR2-were successfully identified. Additionally, ELOVL6, IDI1, and SQLE were co-enriched in multiple pathways, including those associated with graft-versus-host disease (GVHD) in mice. TLR2 expression exhibited the strongest positive correlation with M2 macrophages (ρ = 0.74, P < 0.001) and the strongest negative correlation with neurons (ρ = -0.73, P < 0.001). Protein-ligand interaction analysis showed the highest binding scores of TLR2 with CHEMBL1836411, resveratrol hexanoic acid, and diprovocim-1. Molecular docking further confirmed a strong binding affinity between the TLR2 receptor and these compounds. RT-qPCR demonstrated significantly elevated transcript levels of Fgf2, Il6, Pik3r5, and Tlr2 in SCI samples (P < 0.01), corroborating the bioinformatic predictions. This study identifies FGF2, IL-6, PIK3R5, and TLR2 as key biomarkers in SCI, providing potential therapeutic targets for SCI treatment. - Source: PubMed
Publication date: 2026/04/20
Chen TaibangYu LichaoCai ZhijunChen Lingqiang - Peroxisomes play essential roles in cellular lipid metabolism and redox regulation, yet their contribution to bladder cancer (BLCA) progression remains poorly defined. - Source: PubMed
Publication date: 2026/03/04
Wu QinghuiZhou YuOu ZhewenFu HoushengZeng FanchangLi DaoyuanZheng ZhaocongWang Fei - Ergosterol is the key precursor of steroid drug synthesis. In this experiment, we systematically modified the synthesis of ergosterol. Firstly, we identified key rate-limiting enzymes through systematic screening of the post-squalene pathway. Combinatorial overexpression of , , , , , and achieved an ergosterol titer of 94.2 mg/L. Molecular dynamics guided mutagenesis of key substrate channel residues, particularly S372V Erg11, enhanced local flexibility and significantly increased ergosterol production. Introduction of the proton-donating mutations S372V-T305H- established an artificial proton-dependent pathway, which, together with channel engineering, further increased the titer to 124 mg/L. Lipid droplet engineering and cellular compartmentalization strategies increased the titer to 148.3 mg/L. Ultimately, multi-copy integration of all ergosterol synthesis pathway genes increased the titer to 433.1 mg/L, and fed-batch fermentation in a 5-L bioreactor resulted in a final titer of 4.58 g/L. This study demonstrates a comprehensive hierarchical strategy for high-level sterol production. - Source: PubMed
Publication date: 2026/01/16
Liu XinyuChen QihangZhao WenbaoLi QiXiao LiushutingHe QianZeng WeizhuZhou Jingwen - Idiopathic pulmonary fibrosis (IPF) and sarcopenia significantly affect patients' quality of life. The progression and worsening of these conditions are often associated with endoplasmic reticulum (ER) stress, a key cellular stress-response mechanism. This study aimed to investigate the involvement of ER stress in cellular dysfunction in IPF and sarcopenia by identifying ER stress-related crosstalk genes (ERSRCGs). - Source: PubMed
Publication date: 2025/12/01
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