Ask about this productRelated genes to: AP2B1 antibody
- Gene:
- AP2B1 NIH gene
- Name:
- adaptor related protein complex 2 subunit beta 1
- Previous symbol:
- ADTB2, CLAPB1
- Synonyms:
- -
- Chromosome:
- 17q12
- Locus Type:
- gene with protein product
- Date approved:
- 2000-09-01
- Date modifiied:
- 2018-04-23
Related products to: AP2B1 antibody
Related articles to: AP2B1 antibody
- - Source: PubMed
Publication date: 2026/01/10
Guzman CarlaZhu WenJuanJha AnupmaTong YanjiArrigo AngeloBahar IvetLo CeciliaLin Jiuann-Huey Ivy - Gastric cancer metastasis involves the interaction between tumor cells and their stromal microenvironment. Cancer-associated fibroblasts (CAF) play a pivotal role in this process, and elucidation of the molecular mechanisms underlying gastric cancer cell-CAF interactions could uncover potential therapeutic targets to block metastatic progression. In this study, through transcriptomic profiling of gastric cancer cell-CAF communications, we identified galactose-3-O-sulfotransferase 1 (GAL3ST1) as a key regulator of CAF-induced gastric cancer cell metastatic potential. Mechanistically, GAL3ST1 functioned as a histone sulfotransferase to sulfate nascent histone H3 at tyrosine 99 (H3Y99sulf) in the cytosol of gastric cancer cells. The sulfated histones were subsequently translocated to the nucleus via AP2B1, in which they recruited KAT2A to establish H3K56 acetylation marks that resulted in activation of β-catenin transcription and drove epithelial-mesenchymal transition. Furthermore, CAF-derived SEMA7A engaged ITGB1 on gastric cancer cells and initiated ERK1/2-CEBPB signaling to transcriptionally upregulate GAL3ST1. Collectively, these findings reveal a role for GAL3ST1 in histone sulfation-mediated epigenetic regulation and elucidate the SEMA7A/GAL3ST1/H3Y99sulf axis as a crucial mediator of tumor-stromal cross-talk in gastric cancer metastasis. - Source: PubMed
Lu YifanWu XiongyanLi BaolongYao LizhongHou JunyiYu BeiqinLi JianfangLi ChenYan MinYang ZhongyinYan ChaoZhu ZhengGangLiu BingyaTang KaiwenPan TaoYu ZhenjiaJin ZhijianSu Liping - Profiling the thiol proteome in live cells is a critical yet challenging task for elucidating oxidative stress-related processes due to the scarcity of multifunctional probes that directly integrate fluorescence imaging with chemical proteomics. Here, we constructed a fluorescent, enrichable, and mass spectrometry-compatible probe based on fluorinated porphyrin, termed the FP probe. By eliminating the need for attaching separate reporter modules after probe labeling, the FP probe not only directly visualizes the thiol proteome in cells but also enables the visualization of thiol proteome enrichment for reliable site identification by mass spectrometry. This capability drives the development of a visualization-guided proteomics (VGP) workflow, which seamlessly merges fluorescence imaging with chemical proteomics. In addition, the FP probe demonstrated an advantage in the capture of cysteines with low solvent accessibility. We successfully identified Cys818 of AP2B1, a residue with a relative solvent accessible area of 0.02 that is sensitive to the protein interactions induced by diamide stress in live cells. Our probe may pave the way for the design of multifunctional probes and become an important tool for applications including target identification, drug discovery, and diagnostics. - Source: PubMed
Publication date: 2025/07/05
Liu ZhiyongMa ShiyunZhang YuxiaoYao JunLu Haojie - Aging significantly impacts brain function and increases susceptibility to neurodegenerative diseases, with notable sex dimorphism observed in aging-related dementia, such as Alzheimer's disease. To better understand the molecular mechanisms of aging and dementia, it is essential to globally and accurately characterize biomolecules (e.g., proteins) in the brain as a function of age and sex. Here, we present one of the first studies of aging and sexual dimorphism in brains using the zebrafish (Danio rerio) model, employing mass spectrometry (MS)-based quantitative top-down proteomics (TDP). We analyzed proteoforms in male and female zebrafish brains across three ages (6, 16, and 24 months) using capillary zone electrophoresis-tandem MS (CZE-MS/MS). We revealed significant sex- and age-related differences in the abundance of key proteoforms, including those associated with neurofilament assembly, dopaminergic neuron differentiation, and synaptic vesicle priming. Notably, we identified a truncated proteoform of AP2B1, a subunit of the AP2 adaptor complex closely associated with clathrin-mediated endocytosis and cellular aging, in female zebrafish aged 24 months exclusively, suggesting potential age- and sex-specific roles in brain aging. Additionally, significant changes were observed in proteoforms involved in energy metabolism, structural maintenance, and neurotransmitter release, providing a new opportunity for a better understanding of the molecular mechanisms of brain aging and sexual dimorphism. These findings highlight the effectiveness of CZE-MS/MS in TDP for identifying and quantifying proteoforms, offering a deep view of sex-specific proteoform dynamics during brain aging. - Source: PubMed
Askarani Mehrdad FalamarziPoulos WilliamDashtaki Maryam RahimzadehSadeghi Seyed AmirhosseinCibelli Jose BFang FeiSun Liangliang - Positron emission tomography (PET) imaging with ligands for synaptic vesicle glycoprotein 2A (SV2A) has emerged as a promising methodology for measuring synaptic density in Alzheimer's disease (AD). We investigated the relationship between SV2A PET and CSF synaptic protein changes of AD patients. - Source: PubMed
Nilsson JohannaMecca Adam PAshton Nicholas JSalardini ElahehO'Dell Ryan SCarson Richard EBenedet Andrea LBlennow KajZetterberg Henrikvan Dyck Christopher HBrinkmalm Ann