Ask about this productRelated genes to: TSPYL4 antibody
- Gene:
- TSPYL4 NIH gene
- Name:
- TSPY like 4
- Previous symbol:
- -
- Synonyms:
- dJ486I3.2, KIAA0721
- Chromosome:
- 6q22.1
- Locus Type:
- gene with protein product
- Date approved:
- 2003-06-24
- Date modifiied:
- 2016-07-01
Related products to: TSPYL4 antibody
Related articles to: TSPYL4 antibody
- Chronic venous insufficiency (CVI), a chronic vascular dysfunction, is a common health problem that causes serious complications such as painful varicose veins and even skin ulcers. Identifying the underlying genetic and epigenetic factors is important for improving the quality of life of individuals with CVI. In the literature, many genes, variants, and miRNAs associated with CVI have been identified through genomic and transcriptomic studies. Despite molecular pathogenesis studies, how the genes associated with CVI are regulated by miRNAs and the effect of variants in binding regions on expression levels are still not fully understood. In this study, previously identified genes, variants, and miRNAs associated with CVI, common variants in the mRNA-miRNA binding regions, were investigated using in silico analyses. For this purpose, miRNA research tools, MBS (miRNA binding site) database, genome browsers, and the eQTL Calculator in the GTEx portal were used. We identified SNVs associated with CVI that may play a direct role in the miRNA-mediated regulation of the , , , , , , , , , and genes. In addition, when the common SNVs in the mRNA binding region of 75 unique CVI related-miRNAs in five candidate genes associated with CVI were examined, seven miRNAs associated with the expression profiles of , , and genes were identified. In conclusion, the relationship between genetic markers identified in the literature that play a role in the pathogenesis of the CVI and the expression profiles was evaluated for the first time in the mRNA-miRNA interaction axis. - Source: PubMed
Publication date: 2024/12/31
Sarı-Tunel FatmaDemirkan AyseVural BurcakYıldız Cenk ErayKomurcu-Bayrak Evrim - The objective of this study was to discover autoantibodies to non-modified proteins associated with the presence/absence of ACPAs in RA. - Source: PubMed
Lourido LucíaJoshua VijayHansson MonikaSjöberg RonaldPin ElisaRuiz-Romero CristinaNilsson PeterAlfredsson LarsKlareskog LarsBlanco Francisco J - The cytochromes P450 (CYPs) represent a large gene superfamily that plays an important role in the metabolism of both exogenous and endogenous compounds. We have reported that the testis-specific Y-encoded-like proteins (TSPYLs) are novel gene transcriptional regulators. However, little is known of mechanism(s) by which TSPYLs regulate expression or the functional consequences of that regulation. The gene family includes six members, to . However, is a pseudogene, TSPYL5 is only known to regulates the expression of , and TSPYL6 is expressed exclusively in the testis. Therefore, TSPYL 1, 2 and 4 were included in the present study. To better understand how TSPYL1, 2, and 4 might influence CYP expression, we performed a series of pull-downs and mass spectrometric analyses. Panther pathway analysis of the 2272 pulled down proteins for all 3 TSPYL isoforms showed that the top five pathways were the Wnt signaling pathway, the Integrin signaling pathway, the Gonadotropin releasing hormone receptor pathway, the Angiogenesis pathway and Inflammation mediated by chemokines and cytokines. Specifically, we observed that 177 Wnt signaling pathway proteins were pulled down with the TSPYLs. Subsequent luciferase assays showed that knockdown had a greater effect on the activation of Wnt signaling than did or knockdown. Therefore, in subsequent experiments, we focused our attention on TSPYL1. HepaRG cell qRT-PCR showed that TSPYL1 regulated the expression of CYPs involved in cholesterol-metabolism such as CYP1B1 and CYP7A1. Furthermore, TSPYL1 and β-catenin regulated expression in opposite directions and TSPYL1 appeared to regulate expression by blocking β-catenin binding to the TCF7L2 transcription factor on the promoter. In β-catenin and TSPYL1 double knockdown cells, expression and the generation of CYP1B1 downstream metabolites such as 20-HETE could be restored. Finally, we observed that TSPYL1 expression was associated with plasma cholesterol levels and BMI during previous clinical studies of obesity. In conclusion, this series of experiments has revealed a novel mechanism for regulation of the expression of cholesterol-metabolizing CYPs, particularly CYP1B1, by TSPYL1 Wnt/β-catenin signaling, raising the possibility that TSPYL1 might represent a molecular target for influencing cholesterol homeostasis. - Source: PubMed
Publication date: 2022/11/28
Zhu XiujuanGao HuanyaoQin SisiLiu DuanCairns JunmeiGu YayunYu JiaWeinshilboum Richard MWang Liewei - Cytochrome P450s () display significant inter-individual variation in expression, much of which remains unexplained by known single-nucleotide polymorphisms (SNPs). Testis-specific Y-encoded-like proteins (s) are transcriptional regulators for several drug-metabolizing including However, transcription factors (TFs) that might influence expression through an effect on expression are unknown. Therefore, we studied regulators of expression in hepatic cell lines and their possible SNP-dependent variation. Specifically, we identified candidate TFs that might influence expression using the ENCODE ChIPseq database. Subsequently, the expression of as well as that of selected CYP targets for regulation were assayed in hepatic cell lines before and after knockdown of TFs that might influence expression through TSPYL-dependent mechanisms. Those results were confirmed by studies of TF binding to gene promoter regions. In hepatic cell lines, knockdown of the REST and ZBTB7A TFs resulted in decreased and expression and increased expression, changes reversed by overexpression. Potential binding sites for and on the promoters of and were confirmed by chromatin immunoprecipitation. Finally, common SNP variants in upstream binding sites on the promoters were identified and luciferase reporter constructs confirmed SNP-dependent modulation of gene transcription. In summary, we identified REST and ZBTB7A as regulators of the expression of TSPYL genes which themselves can contribute to regulation of expression and-potentially-of drug metabolism. SNP-dependent modulation of TSPYL transcription may contribute to individual variation in both expression and-downstream-drug response phenotypes. SIGNIFICANCE STATEMENT: Testis-specific Y-encoded-like proteins (s) are transcriptional regulators of cytochrome P450 () gene expression. Here, we report that variation in expression as a result of the effects of genetically regulated TSPYL transcription factors is an additional factor that could result in downstream variation in expression and potentially, as a result, variation in drug biotransformation. - Source: PubMed
Publication date: 2022/09/24
Shivaram SugantiGao HuanyaoQin SisiLiu DuanWeinshilboum Richard MWang Liewei - The coat color of dromedary is usually uniform and varies from black to white, although dark- to light-brown colors are the most common phenotypes. This project was designed to gain knowledge on novel color-related variants using genotyping-by-sequencing (GBS). The association between the SNPs and coat color was tested using MLM (mixed linear models) with kinship matrix. Three GWAS models including white color vs. non-white color, black vs. non-black color, and light-brown vs. dark-brown color were performed. There were no distinct genetic clusters detected based on the color phenotypes. However, admixture occurred among all individuals of the four different coat color groups. We identified nine significant SNPs associated with white color after Bonferroni correction, located close to , and genes in neighboring flanking regions. The 13 significant SNPs associated with black color and the candidate genes were: and . The SNAI1 gene interacted with MCIR, ASIP and KIT genes. These genes play a key role in the melanin biosynthetic and pigmentation biological process and melanogenesis biological pathway. Further research using a larger sample size and pedigree data will allow confirmation of associated SNPs and the identified candidate genes. - Source: PubMed
Publication date: 2022/04/23
Bitaraf Sani MortezaZare Harofte JavadBanabazi Mohammad HosseinFaraz AsimEsmaeilkhanian SaeidNaderi Ali ShafeiSalim NaderTeimoori AbbasBitaraf AhmadZadehrahmani MohammadBurger Pamela AnnaAsadzadeh NaderSilawi MohammadTaghipour Sheshdeh AfsanehMohammad Nazari BehrouzFaghihi Mohammad AliRoudbari Zahra