Ask about this productRelated genes to: NKD1 antibody
- Gene:
- NKD1 NIH gene
- Name:
- NKD inhibitor of WNT signaling pathway 1
- Previous symbol:
- -
- Synonyms:
- -
- Chromosome:
- 16q12.1
- Locus Type:
- gene with protein product
- Date approved:
- 2001-10-19
- Date modifiied:
- 2019-01-25
Related products to: NKD1 antibody
Related articles to: NKD1 antibody
- Genetic improvement of growth traits is a key objective in aquaculture breeding. In this study, we performed whole-genome resequencing of 282 Japanese flounder individuals from a single farmed cohort and obtained approximately 6.67 million high-quality SNPs. Single-trait and multi-trait GWAS were conducted for 11 growth traits: body weight (BW), total length (TL), body length (BL), body depth (BD), trunk length (TUL), head length (HL), snout length (SnL), caudal peduncle depth (CPD), eye diameter (ED), postorbital head length (PoL), and interorbital width (IW). Heritability estimates ranged from 0.172 (IW) to 0.487 (HL). Head length showed the highest heritability, followed by PoL (0.328), ED (0.323), BD (0.300), and CPD (0.291). Genetic correlation analysis revealed that BW was strongly positively correlated with most body size traits ( > 0.9), indicating that simultaneous improvement of these traits is feasible. Single-trait GWAS identified a total of 55 suggestive associated loci (top five SNPs per trait, combined across all 11 traits, at < 1 × 10). Notably, we observed pronounced pleiotropic effects, where a single SNP or gene influences multiple growth traits. For example, multiple SNPs on chromosome 14 were associated with more than five traits (TL, BL, BD, TUL, BW), and the gene affected both head length and post-orbital head length, demonstrating a genetic basis of pleiotropy. Additionally, was associated with head length, and with interorbital width. Multi-trait GWAS uncovered 12 novel loci not detected in single-trait analyses, with candidate gene annotation pointing to pathways such as ECM-receptor interaction (e.g., , ) and Wnt signaling (e.g., ), suggesting that these biological processes may underlie the observed associations. These findings are preliminary and require validation in larger independent cohorts. KEGG enrichment analysis showed significant enrichment in ECM-receptor interaction and non-homologous end-joining pathways (corrected < 0.05). Our results provide a genetic basis for growth trait improvement and candidate markers for breeding programs in Japanese flounder. - Source: PubMed
Publication date: 2026/07/28
Liang ShuoChen AoTuo ZhiliXu JianhuaChen YongJiang Li - 5-Fluorouracil (5-FU) resistance continues to pose a considerable barrier in the postoperative management of advanced colorectal cancer (CRC). This study identified Naked cuticle homolog 1 (NKD1) as a key factor associated with 5-FU resistance in CRC based on sequencing data from the GEO and TCGA databases. Elevated NKD1 expression correlated with adverse clinicopathological features and poor patient survival. Functional assays revealed that NKD1 overexpression elevated the IC50 of 5-FU, promoted tumor cell proliferation, and attenuated apoptosis in both drug-treated and untreated settings. Additionally, NKD1 promoted CRC cell migration and invasion, regulated tumor stem cell markers. Mechanistically, NKD1 stabilizes DVL protein, regulates APC and FZD7, and fosters nuclear accumulation of β-catenin, initiating transcriptional programs downstream of Wnt signaling. Silencing NKD1 synergized with 5-FU to improve therapeutic efficacy in patient-derived organoid and xenograft models. These results establish NKD1 as a key regulator of CRC malignancy and chemoresistance via Wnt/β-catenin pathway activation, supporting its potential as a dual biomarker and therapeutic target in combination regimens. - Source: PubMed
Publication date: 2026/07/10
Dong LingyiYang ZhangquanXu ZelinHuang GuotaoYang XuesiLv YouHuang KunzhaiLiu ZhaohuiZhang FuxingJiang KeweiYu Zhilong - Neoadjuvant immunochemotherapy (nICT) has emerged as a promising strategy for locally advanced gastric cancer (LAGC), yet clinical responses remain heterogeneous and reliable predictive biomarkers are lacking. A comprehensive dissection of the tumor microenvironment (TME) is essential to uncover determinants of therapeutic efficacy and enable precision immunotherapy. - Source: PubMed
Publication date: 2026/05/14
Xu XiaohongFan JunPeng LiXiang ZhengkaiLuo DanjuMa ChenggongHuang BoNie XiuDong Xiaochuan - Prostate cancer (PCa) is a biologically heterogeneous disease that frequently progresses to castration-resistant prostate cancer (CRPC), a challenging clinical stage. The underlying mechanisms driving CRPC progression and resistance to androgen receptor (AR) signaling inhibition (ARSI) remain incompletely understood. Emerging evidence implicates the canonical Wnt pathway as a key contributor to CRPC progression. This study elucidates the role of Wnt pathway activation in mediating resistance to ARSI and identifies a robust molecular signature for predicting treatment outcomes. By integrating genomic and transcriptomic data from PCa patients, patient-derived xenografts (PDXs), and experimental models harboring or not Wnt-activating mutations, we performed differential expression analysis, unsupervised clustering, survival, and viability analysis to assess Wnt/β-catenin pathway activation and its interaction with AR signaling. A specific Wnt transcriptional signature (AXIN2, RNF43, ZNRF3, NKD1) was found to reliably reflect pathway activation in advanced PCa. AR was found to suppress mutation-driven Wnt signaling, which was upregulated upon AR inhibition, contributing to treatment resistance. Targeting β-catenin interactions with co-activators p300/CBP using selective inhibitors (IQ-1 and ICG-001) effectively mitigated Wnt-driven ARSI resistance, restoring sensitivity to therapy in preclinical models. Thus, canonical Wnt pathway activation emerges as a critical mediator of resistance to ARSI in CRPC. The identified Wnt signature holds potential as a biomarker for predicting and monitoring therapeutic outcomes. Concurrent targeting of AR and Wnt signaling represents a promising strategy to overcome treatment resistance, particularly in patients with Wnt-activating mutations. - Source: PubMed
Publication date: 2026/04/14
Anselmino NicolasSanchis PabloBizzotto JuanLabanca EstefaniaDong JiabinShepherd Peter D AYang JunVazquez Elba SMateo JoaquinGueron GeraldineLogothetis Christopher J - As the main nutrient reservoir in cereal grains, the endosperm largely determines grain yield, performance and nutrition. However, knowledge of genes that coordinate endosperm filling and nutrient deposition, which could offer potential for genetic improvement of grain traits, remain limited. Here we identified ZmMYB127, a filling-endosperm-specific MYB transcription factor. Its knockout disrupted filling-stage aleurone layer morphology and nutrient accumulation, leading to reduced kernel weight, quality and nutrition. ZmMYB127 exerts dual transcriptional control over core endosperm-filling genes, including naked endosperm-1/2 (NKD1/2), crinkly4 (CR4) and opaque2 (O2). ZmMYB127 forms an activation complex with O2 to synergistically induce NKD1/2 expression by binding to two distinct cis-regulatory elements (CREs). Conversely, the co-repressor ZmLUG3 bridges ZmMYB127 and ZmABI4, a B3-domain transcription factor, to form a repressive complex that suppresses O2 and CR4 via another CRE pair. Filling-endosperm-specific overexpression of ZmMYB127 enhanced kernel weight, quality and nutrition. Introducing this overexpression into the elite cultivar Zhengdan958 confirmed its breeding potential. Furthermore, its rice homologue OsMYB20 also plays a conserved role in endosperm filling. Our findings establish ZmMYB127 as a promising target for grain improvement without trade-offs for precision breeding. - Source: PubMed
Publication date: 2026/03/19
Shi JianLi ZhiqiangWang ZeyuPan ShuxingWu XuWang XiYe YafengXu ZhuopingHe JunjunZhang Zhiyong