Ask about this productRelated genes to: PHACTR3 antibody
- Gene:
- PHACTR3 NIH gene
- Name:
- phosphatase and actin regulator 3
- Previous symbol:
- C20orf101
- Synonyms:
- PPP1R123
- Chromosome:
- 20q13.32-q13.33
- Locus Type:
- gene with protein product
- Date approved:
- 2001-06-15
- Date modifiied:
- 2018-02-13
Related products to: PHACTR3 antibody
Related articles to: PHACTR3 antibody
- Carcass weight (CW) is a major determinant of beef yield and market value in Korea, yet the genetic basis of this trait remains largely unexplored in cattle from Jeju Island. In this study, we performed a genome-wide association study (GWAS) using both a mixed linear model (MLM) and the FarmCPU approach, followed by pathway and network analyses to identify loci and biological functions underlying CW variation. A total of 256 Jeju cattle (92 Jeju Black and 164 Jeju Black × Hanwoo crossbreds) were initially sampled. One crossbred sample failed genotyping, leaving 255 animals (92 Jeju Black and 163 crossbreds) for analysis. Animals were genotyped using the Illumina BovineSNP50 v3 BeadChip, and 39,055 high-quality single nucleotide polymorphisms (SNPs) were retained after quality control. The MLM analysis detected no genome-wide significant associations, whereas the FarmCPU analysis identified six significant loci on chromosomes 3, 5, 6, 10, and 13, each explaining 2.55-9.58% of the phenotypic variance. Candidate genes located near these loci included , , , , , and two uncharacterized protein-coding genes. Functional enrichment analysis identified biologically relevant pathways including lysine degradation, tryptophan metabolism, glycerolipid metabolism, fatty acid biosynthesis, extracellular matrix-receptor interaction, and signaling cascades such as PI3K-Akt and Rap1, although most pathways were not statistically significant after FDR correction. Protein-protein interaction (PPI) network analysis using STRING highlighted modules of signaling, extracellular matrix, and metabolic genes. These clusters suggest that coordinated interactions among these pathways contribute to carcass growth and development. These findings provide new insights into the molecular basis of CW in Jeju Black and Hanwoo × Jeju Black crossbred cattle and identify candidate genes and pathways that may be useful for genomic selection and the sustainable improvement of Jeju Black cattle populations. - Source: PubMed
Publication date: 2025/11/28
Won MiyoungLee JonganShin Sang-MinLee Seung-EunKim Won-JaeKim Eun-TaeKim Tae-HeePark Hee-BokShokrollahi Borhan - Cervical cancer is preceded by low-grade squamous intraepithelial lesions (LSIL) and high-grade squamous intraepithelial lesions (HSIL). Human papillomavirus (HPV) test is a sensitive method for cervical cancer screening, but it is less specific compared with cytological examination, leading to overtreatment and reduced patient compliance. Therefore, new detection methods that can improve the accuracy of cervical cancer screening are needed. - Source: PubMed
Publication date: 2025/03/13
Ding HuiKe ZhongheXiao XiaoXin BeibeiXiong HuiLu Wen - Human cell reprogramming traditionally involves time-intensive, multistage, costly tissue culture polystyrene-based cell culture practices that ultimately produce low numbers of reprogrammed cells of variable quality. Previous studies have shown that very soft 2- and 3-dimensional hydrogel substrates/matrices (of stiffnesses ≤ 1 kPa) can drive ~2× improvements in human cell reprogramming outcomes. Unfortunately, these similarly complex multistage protocols lack intrinsic scalability, and, furthermore, the associated underlying molecular mechanisms remain to be fully elucidated, limiting the potential to further maximize reprogramming outcomes. In screening the largest range of polyacrylamide (pAAm) hydrogels of varying stiffness to date (1 kPa to 1.3 MPa), we have found that a medium stiffness gel (~100 kPa) increased the overall number of reprogrammed cells by up to 10-fold (10×), accelerated reprogramming kinetics, improved both early and late phases of reprogramming, and produced induced pluripotent stem cells (iPSCs) having more naïve characteristics and lower remnant transgene expression, compared to the gold standard tissue culture polystyrene practice. Functionalization of these pAAm hydrogels with poly-l-dopamine enabled, for the first-time, continuous, single-step reprogramming of fibroblasts to iPSCs on hydrogel substrates (noting that even the tissue culture polystyrene practice is a 2-stage process). Comparative RNA sequencing analyses coupled with experimental validation revealed that a novel reprogramming regulator, protein phosphatase and actin regulator 3, up-regulated under the gel condition at a very early time point, was responsible for the observed enhanced reprogramming outcomes. This study provides a novel culture protocol and substrate for continuous hydrogel-based cell reprogramming and previously unattained clarity of the underlying mechanisms via which substrate stiffness modulates reprogramming kinetics and iPSC quality outcomes. - Source: PubMed
Publication date: 2024/05/21
Chowdhury Mohammad MahfuzZimmerman SamuelLeeson HannahNefzger Christian MaximilianMar Jessica CaraLaslett AndrewPolo Jose MariaWolvetang ErnstCooper-White Justin John - Obesity is an established risk factor for hypertension, but the mechanisms are only partially understood. We examined whether body mass index (BMI)-related DNA methylation (DNAm) variation would mediate the association of BMI with blood pressure (BP). We first conducted a genomewide DNA methylation analysis in monozygotic twin pairs to detect BMI-related DNAm variation and then evaluated the mediating effect of DNAm on the relationship between BMI and BP levels using the causal inference test (CIT) method and mediation analysis. Ontology enrichment analysis was performed for CpGs using the GREAT tool. A total of 60 twin pairs for BMI and systolic blood pressure (SBP) and 58 twin pairs for BMI and diastolic blood pressure (DBP) were included. BMI was positively associated with SBP (β = 1.86, = .0004). The association between BMI and DNAm of 85 CpGs reached < 1×10 level. Eleven BMI-related differentially methylated regions (DMRs) within , , , , , , , , , and were found. Of the 85 CpGs, 9 mapped to , , , , , and were significantly associated with SBP levels. Of the 9 CpGs, 2 within negatively mediated the association between BMI and SBP, with a mediating effect of -0.24 (95% CI [-0.65, -0.01]). BMI was also positively associated with DBP (β = 0.60, = .0495). The association between BMI and DNAm of 193 CpGs reached < 1×10 level. Twenty-five BMI-related DMRs within , , , , , , and were found. Of the 193 CpGs, 33 mapped to , , , , , , and were significantly associated with DBP levels. Of the 33 CpGs, 12 within , , , , , , and negatively mediated the association between BMI and DBP, with a total mediation effect of -0.66 (95% CI [-1.07, -0.30]). Interestingly, BMI might also negatively mediate the association between the DNAm of most CpG mediators mentioned above and BP. The mediating effect of DNAm was also found when stratified by sex. In conclusion, DNAm variation may partially negatively mediate the association of BMI with BP. Our findings may provide new clues to further elucidate the pathogenesis of obesity to hypertension and identify new diagnostic biomarkers and therapeutic targets for hypertension. - Source: PubMed
Publication date: 2024/01/31
Yao JieNing FengWang WeijingZhang Dongfeng - Few genetic polymorphisms predict early response to anti-TNF drugs in inflammatory bowel disease (IBD), and even fewer have been identified in the pediatric population. However, it would be of considerable clinical interest to identify and validate genetic biomarkers of long-term response. Therefore, the aim of the study was to analyze the usefulness of biomarkers of response to anti-TNFs in pediatric IBD (pIBD) as long-term biomarkers and to find differences by type of IBD and type of anti-TNF drug. The study population comprised 340 children diagnosed with IBD who were treated with infliximab or adalimumab. Genotyping of 9 selected SNPs for their association with early response and/or immunogenicity to anti-TNFs was performed using real-time PCR. Variants C rs10508884 (CXCL12), A rs2241880 (ATG16L1), and T rs6100556 (PHACTR3) (p value 0.049; p value 0.03; p value 0.031) were associated with worse long-term response to anti-TNFs in pIBD. DNA variants specific to disease type and anti-TNF type were identified in the pediatric population. Genotyping of these genetic variants before initiation of anti-TNFs would enable, if validated in a prospective cohort, the identification of pediatric patients who are long-term responders to this therapy. - Source: PubMed
Publication date: 2023/07/19
Zapata-Cobo PaulaSalvador-Martín SaraVelasco MartaPalomino Laura MClemente SusanaSegarra OscarMoreno-Álvarez AnaFernández-Lorenzo AnaPérez-Moneo BegoñaMontraveta MontserratSánchez CesarTolín MarLoverdos InésFobelo María JesúsNavas-López Victor ManuelMagallares LorenaGarcía-Romero RuthSánchez-Hernández José GermánRodríguez AlejandroBossacoma FerránBalboa María JesúsSalcedo EnriqueSanjurjo-Sáez MaríaLópez-Fernández Luis A