Ask about this productRelated genes to: H2AFY antibody
- Gene:
- H2AFY NIH gene
- Name:
- H2A histone family member Y
- Previous symbol:
- -
- Synonyms:
- macroH2A1.2
- Chromosome:
- 5q31.1
- Locus Type:
- gene with protein product
- Date approved:
- 1999-07-22
- Date modifiied:
- 2015-11-18
Related products to: H2AFY antibody
Related articles to: H2AFY antibody
- Patients with juvenile idiopathic arthritis (JIA) frequently exhibit antinuclear antibodies (ANAs), but the specific antigen target recognized by them and the presence of additional autoantibody specificities in patients with JIA remains elusive. - Source: PubMed
Publication date: 2025/09/21
Pitkänen ViolaRemes-Pakarinen TerhiVähäsalo PaulaMöttönen MiljaGrönlund Minna-MaijaArvonen MiikaKnip MikaelVeijola RiittaToppari JormaIlonen JormaLempainen JohannaSchroderus Anna-MariKröger LiisaKinnunen Tuure - Feathered foot is a trait observed in domestic and wild chickens, resulting from the partial or complete transformation of scales on the tarsus, shanks, and toes into feathers. Although previous studies have mapped genomic loci associated with feathered feet, the molecular mechanisms underlying this transformation remain unclear. This study combined whole-genome re-sequencing (WGS) data, transcriptomic analysis, and cellular experiments to investigate the genetic basis of feathered feet in Guangxi native chickens. A genome-wide association study (GWAS) involving 1,735 Guangxi native chickens identified two genomic regions and several candidate genes, including TBX3, TBX5, and H2AFY. RNA sequencing (RNA-seq) and real-time fluorescent quantitative PCR (QRT-PCR) results confirmed that TBX5 is differentially expressed between chickens with scaled and feathered feet, indicating its role as a key candidate gene for feathered feet. The function of TBX5 was subsequently explored at the cellular level using chicken dermal fibroblasts. The results showed that moderate overexpression of TBX5 (20 ng) significantly increased the expression of cell proliferation-related genes. In contrast, excessive overexpression of TBX5 (2.5 μg) and interference with TBX5 inhibited these genes, suggesting a dose-dependent effect of TBX5 on the proliferation of dermal fibroblasts. EdU fluorescence staining, flow cytometry, and migration assays demonstrated that moderate expression of TBX5 can promote the proliferation and migration of dermal fibroblasts, while TBX5 interference produced opposite results. Our findings suggest that TBX5 is a pivotal candidate gene associated with feathered feet in Guangxi native chickens. It is proposed that TBX5 may affect the formation of feathered feet by regulating the proliferation and migration of dermal fibroblasts. - Source: PubMed
Publication date: 2025/02/01
Yang ZhuliangLiu YongcuiLuo JintangXiao CongYuan HongDu WenyaYang Xiurong - Childhood neuroblastoma with MYCN amplification is classified as high risk and often relapses after intensive treatments. Immune checkpoint blockade therapy against the PD-1/L1 axis shows limited efficacy in patients with neuroblastoma, and the cancer intrinsic immune regulatory network is poorly understood. Here, we leverage genome-wide CRISPR/Cas9 screens and identify H2AFY as a resistance gene to the clinically approved PD-1 blocking antibody nivolumab. Analysis of single-cell RNA-Seq datasets reveals that H2AFY mRNA is enriched in adrenergic cancer cells and is associated with worse patient survival. Genetic deletion of H2afy in MYCN-driven neuroblastoma cells reverts in vivo resistance to PD-1 blockade by eliciting activation of the adaptive and innate immunity. Mapping of the epigenetic and translational landscape demonstrates that H2afy deletion promotes cell transition to a mesenchymal-like state. With a multiomics approach, we uncovered H2AFY-associated genes that are functionally relevant and prognostic in patients. Altogether, our study elucidates the role of H2AFY as an epigenetic gatekeeper for cell states and immunogenicity in high-risk neuroblastoma. - Source: PubMed
Publication date: 2024/09/10
Nagarajan DivyaParracho Rebeca TCorujo DavidXie MingluKutkaite GinteOlsen Thale KRubies Bedos MartaSalehi MaedeBaryawno NinibMenden Michael PChen XingqiBuschbeck MarcusMao Yumeng - Circulating tumor cell (CTC) clusters exhibit significantly higher metastatic potential compared to single CTCs. However, the underlying mechanism behind this phenomenon remains unclear, and the role of posttranscriptional RNA regulation in CTC clusters has not been explored. Here, we conducted a comparative analysis of alternative splicing (AS) and alternative polyadenylation (APA) profiles between single CTCs and CTC clusters. We identified 994 and 836 AS events in single CTCs and CTC clusters, respectively, with ∼20% of AS events showing differential regulation between the two cell types. A key event in this differential splicing was observed in SRSF6, which disrupted AS profiles and contributed to the increased malignancy of CTC clusters. Regarding APA, we found a global lengthening of 3' UTRs in CTC clusters compared to single CTCs. This alteration was primarily governed by 14 core APA factors, particularly PPP1CA. The modified APA profiles facilitated the cell cycle progression of CTC clusters and indicated their reduced susceptibility to oxidative stress. Further investigation revealed that the proportion of H2AFY mRNA with long 3' UTR instead of short 3' UTR was higher in CTC clusters than single CTCs. The AU-rich elements (AREs) within the long 3' UTR of H2AFY mRNA enhance mRNA stability and translation activity, resulting in promoting cell proliferation and invasion, which potentially facilitate the establishment and rapid formation of metastatic tumors mediated by CTC clusters. These findings provide new insights into the mechanisms driving CTC cluster metastasis. - Source: PubMed
Publication date: 2024/02/27
Wu QuanyouGu ZhaoruShang BingqingWan DuoZhang QiZhang XiaoliXie PeipeiCheng ShujunZhang WenZhang Kaitai - Breast cancer (BRCA) has a high incidence and mortality rate among women. Different molecular subtypes of breast cancer have different prognoses and require personalized therapies. It is imperative to find novel therapeutic targets for different molecular subtypes of BRCA. Here, we demonstrated for the first time that Cytochromeb561 (CYB561) is highly expressed in BRCA and correlates with poor prognosis, especially in HER2-positive BRCA. Overexpression of CYB561 could upregulate macroH2A (H2AFY) expression in HER2-positive BRCA cells through inhibition of H2AFY ubiquitination, and high expression of CYB561 in HER2-positive BRCA cells could promote the proliferation and migration of cells. Furthermore, we have demonstrated that CYB561 regulates H2AFY expression, thereby influencing the expression of NF-κB, a downstream molecule of H2AFY. These findings have been validated through in vivo experiments. In conclusion, we propose that CYB561 may represent a novel therapeutic target for the treatment of HER2-positive BRCA. Graphical abstract CYB561 promotes the proliferation of HER2+ BRCA cells: CYB561 enhances the expression of H2AFY by inhibiting its ubiquitination, which leads to an increase expression of NF-κB in the nucleus. H2AFY, together with NF-κB, promotes the proliferation of HER2+ BRCA cells. - Source: PubMed
Publication date: 2024/01/20
Zhao TingWang ChaominZhao NaQiao GeHua JialeiMeng DonghuaLiu LimingZhong BenfuLiu MiaoWang YichaoBai ChangsenLi Yueguo