Ask about this productRelated genes to: SLA1 antibody
- Gene:
- SLA NIH gene
- Name:
- Src like adaptor
- Previous symbol:
- -
- Synonyms:
- SLA1, SLAP-1, hSLAP, SLAP
- Chromosome:
- 8q24.22
- Locus Type:
- gene with protein product
- Date approved:
- 1995-08-11
- Date modifiied:
- 2016-12-13
Related products to: SLA1 antibody
Related articles to: SLA1 antibody
- Macrophages are central players of innate immunity with diverse functions and involvement in numerous diseases, making it essential to understand their metabolic regulation. Here, we provide a comprehensive analysis of lipid metabolic dynamics during human monocyte-to-macrophage differentiation. Primary blood monocytes were differentiated for 5 days with either M-CSF or GM-CSF. Lipidomic profiling using the differential mobility spectrometry-shotgun lipidomics assistant (DMS-SLA) platform reliably quantified ∼400 lipids across 16 classes, while spectral flow cytometry was used to assess metabolic enzyme expression. Differentiation was marked by remodeling toward membrane lipids, accompanied by shorter acyl chains and reduced unsaturation in triglyceride (TG) and phosphatidylcholine (PC) species. PC species incorporated a more diverse range of pro- and anti-inflammatory precursors, whereas TG species mainly incorporated fatty acid FA 22:6. Metabolic enzyme expression showed dynamic, marker-specific changes over time, with G6PD and SDHA upregulation indicating enhanced pentose phosphate and oxidative phosphorylation pathways. M-CSF MDMs exhibited higher GLUT1 and CD36 expression and greater FA 22:6 incorporation within TGs. Together, these findings reveal extensive lipidome remodeling that underlies macrophage differentiation and distinct functional states. - Source: PubMed
Publication date: 2026/05/28
Bacon AliceAlmeida LuisGhorasaini MohanHafkenscheid LiseToes Rene E MEverts BartGiera Martin - Cystic fibrosis (CF) is a genetic disorder marked by impaired chloride transport, with elevated sweat chloride concentration serving as the primary diagnostic biomarker. Here, we report a 3D-printed chloride-selective ion-selective electrode (3Dp-Cl-ISE) for sweat chloride analysis relevant to CF screening. This work introduces a solvent-free, 3D-printed solid-contact chloride ISE that integrates a photocurable non-polyvinyl chloride selective membrane with a carbon-cloth transducer. The sensor combines a stereolithography (SLA)-printed membrane with a porous, hydrophobic carbon cloth substrate that functions as a capacitive electron reservoir, promoting efficient ion-to-electron transduction, suppressing water-layer formation, and enhancing potential stability. The fully integrated 3Dp-Cl-ISE exhibits a Nernstian response (-55.3 mV/decade) over a physiologically relevant chloride concentration range (15.6-250 mM), covering the chloride range relevant to CF sweat-test interpretation. Selectivity studies demonstrate minimal interference from common sweat anions, including bicarbonate and lactate. The sensor was evaluated in commercially sourced human sweat using spike-and-recovery experiments across chloride concentrations relevant to CF screening, accurately quantifying spiked chloride concentrations spanning the clinically relevant screening range, including concentrations above 60 mM Cl. Together, these results demonstrate a durable, low-drift, and sweat-compatible sensing platform that leverages additive manufacturing to enable customizable, low-cost, and scalable chloride sensing, highlighting its potential for future point-of-care implementation and noninvasive monitoring of cystic fibrosis. - Source: PubMed
Publication date: 2026/05/26
Farahani SarahHille Nathaniel HSireesha PedaballiBell Jeffrey G - Amyotrophic lateral sclerosis (ALS) is a rapidly progressive and fatal neurodegenerative disorder with marked biological heterogeneity. Despite extensive research, reliable prognostic biomarkers remain limited, with neurofilament light chain (NfL) being the only marker increasingly implemented in clinical practice. The objective of this study is to assess and compare the prognostic value of NfL, circulating markers of central nervous system (CNS) barrier dysfunction, inflammatory mediators, kynurenine pathway metabolites, and global metabolomic profiles in patients with ALS. Seventy-two patients with ALS from the prospective multicenter METABALS cohort were included. Serum, cerebrospinal fluid (CSF), and urine samples were collected at diagnosis. NfL concentrations, markers of blood-brain and blood-spinal cord barrier permeability (albumin quotient, S100B, neuron-specific enolase [NSE]), 48 inflammatory mediators, kynurenine pathway metabolites, and untargeted metabolomic profiles were measured. Associations with clinical features, disease progression, and survival were investigated using univariate analyses and multivariate models. Serum and CSF NfL concentrations were strongly associated with ALS Functional Rating Scale-Revised scores, respiratory function, diagnostic delay, and survival. Higher serum NfL concentrations at diagnosis predicted shorter survival (ROC AUC = 0.86). In all multivariate and multi-block models, serum NfL was the only biomarker independently associated with survival. Markers of CNS barrier integrity, inflammatory mediators, and metabolomic signatures showed limited prognostic value but provided insights into metabolic remodeling and barrier dysfunction. In this integrated multi-omics study, serum NfL clearly outperformed inflammatory, metabolic, and CNS barrier markers as a prognostic biomarker in ALS, supporting its central role in clinical stratification while complementary biological markers highlighted several relevant pathophysiological mechanisms. - Source: PubMed
Publication date: 2026/05/28
Alarcan HugoVeyrat-Durebex CharlottePradat Pierre-FrançoisCassereau JulienDestee AlainCouratier PhilippeCamu WilliamNeau Jean-PhilippeFleury-Lesaunier Marie-CélineEmond PatrickDufour DianeAl Ojaimi YaraLefèvre AntoineVourc'h PatrickCorcia PhilippeAndres Christian RBlasco Hélène - In the European Union (EU), donanemab is indicated in adults with early symptomatic Alzheimer's disease who are apolipoprotein E ε4 non-carriers or heterozygotes. Among these, patients without superficial siderosis at baseline, uncontrolled hypertension, or anticoagulant use are eligible. - Source: PubMed
Publication date: 2026/05/27
Jessen FrankDell'Agnello GraziaZimmer Jennifer ASapin ChristopheDichter SaschaDoty ErinEpelbaum StéphaneEvans Cynthia DHauck Paula MKhanna RashnaBrooks Dawn ASims John RAgosta Federica - Protein synthesis is a crucial biosynthetic process in all organisms, including plants. The integrity of the translational machinery, especially ribosomes, can be compromised during rapid cell division in ontogenesis or in response to environmental stress. In this study, Northern blotting was employed to analyze total RNA from various angiosperms, focusing on small 5'- and 3'-terminal 18S rRNA fragments. Stem-loop array RT-PCR was employed to map the cleavage sites within the target regions. Severe stress, such as extreme drought, induced the accumulation of three distinct 18S rRNA fragments across diverse angiosperm taxa, indicating that this phenomenon is likely universal. In rapidly dividing cells, such as those found in in vitro callus cultures and germinating wheat embryos, high levels of discrete 5'-terminal fragments were observed, while 3'-terminal fragments were absent. The stem-loop array RT-PCR mapping identified specific sites of 18S rRNA strand breaks. Structural annotation of the 3D model of the plant 40S subunit revealed spatial clustering of these sites in proximity to the RPS6 binding region. Notably, wheat cultivars that are tolerant to osmotic stress exhibited significantly higher levels of 18S rRNA fragmentation than sensitive cultivars. This suggests a regulatory mechanism rather than a mere byproduct of apoptotic-like regulated cell death. Additionally, fragmented ribosomes were gradually eliminated during embryo maturation, indicating a process of programmed functional ribophagy. Our findings suggest that a potential inability of plant tissues to selectively retain functional ribosomes might contribute to a decline in generative potential. Monitoring the integrity of the translational machinery could improve breeding efficiency and aid in preserving long-term stored germplasm. - Source: PubMed
Publication date: 2026/05/15
Malysheva Angelina ALopatchenko Taissiya SOsikova Kamilla GKan TatyanaNizkorodova Anna SKryldakov Ruslan VIskakov Bulat KZhigailov Andrey V