Ask about this productRelated genes to: TIP47 antibody
- Gene:
- PLIN3 NIH gene
- Name:
- perilipin 3
- Previous symbol:
- M6PRBP1
- Synonyms:
- TIP47, PP17
- Chromosome:
- 19p13.3
- Locus Type:
- gene with protein product
- Date approved:
- 2004-01-16
- Date modifiied:
- 2014-11-19
Related products to: TIP47 antibody
Related articles to: TIP47 antibody
- Exposure to high and sustained levels of non-esterified fatty acids (NEFA) in the peripartal period is the main cause of fatty liver disease in dairy cows. Rumen-protected choline is often fed as part of the nutritional management of peripartal cows, with in vivo and in vitro data indicating positive effects of this nutrient on alleviating liver lipid accumulation. Although hepatic molecular mechanisms associated with choline supply have been studied using a target gene, protein, or metabolite approach, application of high-throughput technologies could vastly enhance fundamental knowledge on the functional role of choline. The main objective was to challenge isolated hepatocytes with a mixture of NEFA and determine proteome- and metabolome-wide effects in response to choline supply. Three healthy female calves (1 d old, 30-45 kg) were sacrificed to harvest hepatocytes. During a 12 h incubation, isolated hepatocytes were challenged without NEFA (control), 1.2 mM NEFA (c9-18:1, 18:2, 16:0, 18:0, and c9-16:1 at 43.5%, 4.9%, 31.9%, 14.4%, and 5.3% of total NEFA, respectively), or NEFA for 6 h followed by 10 μM choline chloride for another 6 h (NEFA + Chol). iTRAQ labeling-based protein profiling and GC/MS-based metabolomics profiling were used to determine changes in proteins and metabolites. Differentially abundant proteins for each group comparison were determined at a threshold of 1.4-fold change. Differences in metabolite profiles were assessed via pairwise comparisons. A subset of differentially abundant proteins was validated via qRT-PCR and Western blotting. Compared with the control, there were 90 proteins and 22 metabolites in the NEFA group, and 83 proteins and 29 metabolites in the NEFA + Chol. Compared with NEFA, there were 49 proteins and 17 metabolites in the NEFA + Chol group. Greater abundance of hexokinase-1 (HK1), fructose-bisphosphate aldolase (ALDOA), mitochondrial pyruvate carrier 1 (MPC1), and increased concentrations of lactate with high NEFA treatment alone suggested greater glycolytic and TCA cycle activity. Accumulation of triacylglycerol in the NEFA group was associated with lipotoxicity and markers of inflammation, such as greater abundance of prostaglandin reductase 1 (PTGR1), serious cell autophagy processes, such as greater abundance of cell division cycle 42 (CDC42), and NFκB-related proteins. Choline supplementation reduced TAG partly due to greater VLDL secretion driven by greater abundance of diacylglycerol acyltransferase (DGAT1), perilipin 3 (PLIN3), and apolipoprotein C-III (APOC3). In addition, a greater abundance of carnitine O-palmitoyltransferase 1b (CPT1B) with choline suggested enhanced mitochondrial β-oxidation. Activation of the CDC42/JNK pathway and ROS/NFκB axis-related proteins, along with depressed PI3K/AKT/RAC-related proteins, indicated enhanced mitochondrial autophagy in response to NEFA. Overall, data confirmed published effects of choline on TAG accumulation, VLDL secretion, and fatty acid oxidation, while highlighting negative effects of NEFA on the respiratory electron transport chain, autophagy, and inflammatory processes. - Source: PubMed
Publication date: 2026/06/26
Chang YaqiJia BinSi YaranZhang ZexinLiu JiachenGao YueWang JunhaoWang YanhuiLoor Juan JZhang BingbingYang Wei - Recently, immune checkpoint inhibitors (ICIs), especially those that targets PD-1/PD-L1, have significantly altered the treatment approach for NSCLC. Nevertheless, many patients experience different levels of resistance after receiving treatment with ICIs, which restrict their broader use in clinical practice. Therefore, to enhance the overall efficacy of ICIs, there is an immediate necessity to further explain the processes of immune evasion in NSCLC, especially the modulatory mechanism of PD-L1. - Source: PubMed
Publication date: 2026/07/23
Zhou HanqiongFan SongqingOuyang MinZheng Hongmei - Huaier, a traditional Chinese medicinal mushroom, has been shown to have anti-tumor properties in our previous studies. However, its role and underlying mechanisms in regulating doxorubicin (DOX) sensitivity in triple-negative breast cancer (TNBC) remain unclear. - Source: PubMed
Publication date: 2026/07/09
Ma TingtingNiu TongLi YamingYang Qifeng - Reducing reliance on supplemental fish oil is central to sustainable aquaculture, but the molecular consequences of replacing it with vegetable oils remain poorly characterized in the juvenile Chinese soft-shelled turtle (). We evaluated whether full substitution of the supplemental dietary fish oil (FO) with linseed oil (LO) or soybean oil (SO) compromises hepatic lipid metabolism in . Three isonitrogenous and isolipidic diets, sharing identical fish meal and other ingredient bases and differing only in the supplemental lipid (4% FO, LO or SO), were fed to triplicate groups of juvenile turtles (initial body weight 55.0 ± 0.05 g) for 8 weeks. Growth performance, survival, feed conversion ratio, and serum biochemistry were unaffected. However, both vegetable oil diets altered tissue fatty acid composition, raising n-6 PUFA and lowering n-3 LC-PUFA and the n-3/n-6 ratio in liver and muscle (muscle EPA and DHA each decreased by approximately 40%); the SO group additionally exhibited elevated hepatic malondialdehyde, whereas hepatic lipid droplet area and lipid content did not differ significantly among groups. Liver transcriptomic profiling identified 262 (LO vs. FO) and 214 (SO vs. FO) differentially expressed genes, converging on lipid storage and bile acid metabolism. RT-qPCR confirmed the up-regulation of , and and the down-regulation of . Over 8 weeks, replacement of supplemental FO maintained growth without overt impairment while altering tissue fatty acid profiles and the hepatic expression of key lipid metabolism genes. - Source: PubMed
Publication date: 2026/07/02
Li RuiGuo YileiZhao EnhaoGe ChutianSun Jie - Hepatic stellate cell activation is characterized by loss of cytoplasmic lipid droplets and acquisition of a profibrogenic phenotype; however, the contribution of lipid droplet-associated perilipins remains poorly understood. We investigated differential perilipin modulation during TGFβ1 induced activation of LX-2 cells and whether lipid storage restoration could reverse altered perilipin expression and fibrogenic features. TGFβ1 stimulation induced enhanced proliferation, fibroblast-like morphology, reduced neutral lipid accumulation, and increased fibrotic marker expression, associated with a distinct perilipin remodelling pattern: suppression of PLIN2 and induction of PLIN3 and PLIN5, indicating selective adaptation of LD machinery. Oleic acid supplementation partially restored intracellular LDs, selectively increased PLIN2, and showed differential effects on PLIN3 and PLIN5, with a trend toward reduced fibrotic markers, though not statistically significant. These findings suggest perilipin remodelling reflects metabolic adaptation in activated HSCs, and LD restoration alone is insufficient to reverse fibrogenic activation. - Source: PubMed
Publication date: 2026/07/02
Franco P SSahu GhanshyamMahawar ManishRoeb ElkeIrungbam Karuna