Ask about this productRelated genes to: FGF10 antibody
- Gene:
- FGF10 NIH gene
- Name:
- fibroblast growth factor 10
- Previous symbol:
- -
- Synonyms:
- -
- Chromosome:
- 5p12
- Locus Type:
- gene with protein product
- Date approved:
- 1996-12-16
- Date modifiied:
- 2016-10-05
Related products to: FGF10 antibody
Related articles to: FGF10 antibody
- Thyroid hormones (THs) regulate embryonic growth and tissue remodeling, yet their contribution to avian limb morphogenesis remains incompletely defined. We hypothesized that amiodarone-induced inhibition of TH signaling can disrupt chick hindlimb development by altering key patterning pathways and epithelial-mesenchymal dynamics during early limb bud morphogenesis. Fertilized Rhode Island Red eggs received in ovo air-cell injections of phosphate-buffered saline (control) or amiodarone (1 mM) at embryonic day 0 and were analyzed at days 2, 4, and 10, with some embryos allowed to hatch for phenotypic assessment. Hindlimb development was evaluated using morphological, histological, molecular, cellular, and biochemical approaches, including gene and protein expression analyses, apoptosis and cell-cycle profiling, systems-level pathway analyses, and tissue remodeling endpoints. Amiodarone exposure produced consistent limb deformities at hatching and impaired chondrogenic organization at day 10. Molecular profiling revealed elevated SHH/FGF8/WNT3/HOXA10/CASP3 with concomitant suppression of hindlimb identity and mesenchymal/EMT-associated genes (TBX4, PITX1, FGF10, GLI3, SNAIL, WNT7A). Protein and flow cytometry data supported uncoupling of the AER-mesenchyme axis, increased epithelial CDH1-positive populations with reduced SNAIL, and enhanced cleaved Caspase-3 associated apoptosis, corroborated by expanded Nile Blue staining. Proteomic shifts, reduced acetylcholinesterase activity, and decreased hydroxyproline further suggest disrupted differentiation and matrix maturation. Collectively, these results suggest that intact TH signaling might be essential for coordinating patterning cues, epithelial-mesenchymal balance, cell survival, and extracellular matrix maturation during chick hindlimb development, and that amiodarone exposure is associated with disruption of these integrated developmental programs, which may contribute to the observed structural limb defects. - Source: PubMed
Publication date: 2026/09/07
Vaishnav JuhiBalakrishnan Suresh - The gliding patagium represents a key adaptation for mammalian flight, but its cellular development remains unexplored. Using single-nucleus RNA sequencing of embryonic flying squirrel patagium and dorsal skin, we construct a single-cell atlas of patagium development and identify two distinct fibroblast subpopulations (Fp2 and Fr) highly enriched in the patagium. These fibroblasts are characterized by the patagium upregulation of Wnt5a, Fgf7, and Fgf10, and are associated with patagium morphogenesis through dermal-epidermal putative communication interactions between dermal fibroblasts (Fp2 and Fr) and epithelial basal keratinocytes. Specifically, Fp2 fibroblasts are potentially involved in distal dermal condensation and epithelial thickening together with elevated Wnt5a expression, while both Fp2 and Fr fibroblasts could play a role in epithelial polarization and thickening through Fgf7 and Fgf10, as suggested by ex vivo assays. Our data suggest that gliding patagium development results from the co-option of conserved WNT and FGF signaling pathways within a specialized fibroblast-epithelial context, illustrating how modifications of conserved developmental programs give rise to derived morphological traits. - Source: PubMed
Publication date: 2026/08/04
Lin YouFuHuang ShuangXianChe LiFengZhao HaoZheWang YaJunYang MingFeiAi ChengRuan JueWang XiaoPingYu Li - CRISPR/Cas9-mediated genome editing enables efficient generation of knockout mouse models; however, frameshift mutations do not always result in complete loss of function. The factors influencing functional inactivation following frameshift mutations remain incompletely understood. Here, we tested whether frameshift-dominant targeting of exon 1 is sufficient to generate a null allele of , a gene essential for limb formation. - Source: PubMed
Publication date: 2026/08/01
Lkhagvadorj KhanuiOkamura EiichiMorimura ToshifumiMizuno SeiyaEma Masatsugu - Establishing sensitive and reliable methods for the quantification of structurally intact recombinant protein therapeutics in biological matrices remains essential for pharmacokinetic studies but continues to be analytically challenging. In this study, we developed an aptamer-based magnetic solid-phase extraction coupled with liquid chromatography-tandem mass spectrometry (Apt-MSPE-LC-MS/MS) method for the quantitative determination of recombinant fibroblast growth factor 10 (rFGF10) in rat plasma. The aptamer-mediated extraction enabled selective enrichment of rFGF10 prior to LC-MS/MS analysis, resulting in a 7.2-fold increase in analytical sensitivity compared with direct LC-MS/MS analysis. The developed method exhibited satisfactory linearity over a concentration range of 1.0-50 μg⋅mL⁻¹ (R² = 0.998), with acceptable precision (6.3-13.6%), accuracy (%Bias, -5.8% to 8.0%), and a limit of detection (LOD) of 0.53 μg⋅mL⁻¹. Matrix effect evaluation demonstrated acceptable and reproducible matrix effects, with coefficient of variation (CV) values below 15%. The validated Apt-MSPE-LC-MS/MS method was successfully applied to the pharmacokinetic analysis of rFGF10 in rats. Overall, this workflow provides a selective and reliable strategy for the quantitative analysis of structurally intact recombinant proteins in complex biological matrices. - Source: PubMed
Publication date: 2026/08/20
Cai XiaojunXie XuXie YileYu YangLi ZhouChen XiangzhunZhou ZhiyuanCai YuepiaoYang JinmeiJin Lei - Rex rabbit fur quality determines economic value, yet the age-dependent dynamics of hair follicle development and their molecular drivers are largely unknown. Here, we performed a time-series integrative analysis combining phenotypic measurements, quantitative histomorphology, and transcriptome sequencing in Yongqing Rex rabbits across 1 to 6 months of age (10 rabbits per month). Fur thickness exhibited a pattern of increase, stabilization, and subsequent increase, with no significant differences between 2M and 4M (p > 0.05). Coat density peaked at 6M, with an average pelt area of 1358.20 ± 52.43 cm at 5M and 6M. Fiber length increased and then plateaued, whereas hair diameter showed an increase-decrease-stabilization pattern. Histological analysis revealed a significant age-related decrease in the number of follicle clusters, particularly from 1M to 3M (p < 0.05). Primary hair follicle density was the highest at 1M (p < 0.01) and fluctuated subsequently, whereas secondary hair follicle density increased continuously (p < 0.01). Melanin granules increased significantly from 1M to 4M and peaked at the 4M (p < 0.05). Further, transcriptomic identified 1168, 4204, and 3431 differentially expressed genes (DEGs) for 3M versus 1M, 5M versus 1M, and 5M versus 3M, respectively, with 187 common DEGs including FOXN1, FGF10, and DCN. GO and KEGG analysis indicated significant enrichment in processes such as keratinization, keratinocyte differentiation, ECM-receptor interaction, and the PI3K-Akt signaling pathway. Collectively, our findings reveal the dynamic patterns of hair follicle development in Rex rabbits across phenotypic, histological, and molecular levels, providing a theoretical basis for the genetic regulation of fur quality. - Source: PubMed
Cao ChengpengYang JingyiWang YanlinWang HaoyueWang LeiZhao BohaoWu XinshengLiu YanChen Yang