Ask about this productRelated genes to: BMP7 antibody
- Gene:
- BMP7 NIH gene
- Name:
- bone morphogenetic protein 7
- Previous symbol:
- -
- Synonyms:
- OP-1
- Chromosome:
- 20q13.31
- Locus Type:
- gene with protein product
- Date approved:
- 1991-06-05
- Date modifiied:
- 2016-10-05
Related products to: BMP7 antibody
Related articles to: BMP7 antibody
- Sclerostin (SOST) is a secreted glycoprotein that antagonizes Wnt and BMP signaling, however, the molecular mechanisms regulating its extracellular distribution and growth factor (GF) interactions remain poorly understood. Here, we identify fibrillin microfibrils as high-affinity extracellular binding partners of SOST. Recombinant SOST bound specifically to fibrillin-1 positive fibers in cell culture and localized near the bead region of purified tissue fibrillin microfibrils. Surface plasmon resonance (SPR) mapping revealed that SOST recognizes the N-terminal regions of fibrillin-1 and fibrillin-2 with nanomolar affinity, and deletion analysis identified the fibrillin-unique N-terminal (FUN) domain as the primary SOST binding site. Our findings further extend the SOST binding repertoire to include BMP-10 GF, as well as the prodomains (PDs) and PD-GF complexes (CPLXs) of BMP-7 and BMP-10. Heparin interfered with SOST binding to BMP-7 PDs and CPLXs but not with the SOST-fibrillin-1 interaction. SPR interaction experiments showed that SOST binding to fibrillin-1 occludes the BMP PD targeting site and that fibrillin-1-bound SOST is unable to engage BMP-7 PDs. Molecular docking further supported this competitive mechanism by revealing overlapping interaction interfaces among SOST, BMP-7 CPLX, and fibrillin-1. Together, these findings establish SOST as a multifunctional ECM-associated BMP modulator that links fibrillin microfibril scaffolds to the fine-tuning of BMP and Wnt signaling. - Source: PubMed
Publication date: 2026/09/29
Correns AnnkatrinMarcous FadyLütke SteffenMörgelin MatthiasBarnowski PhilippFurlan Ariane GSchröder Nathalie HLevkau BodoRiek SarahMollenhauer MartinKoch ManuelSengle Gerhard - : Prostate cancer (PCa) is a leading cause of cancer-related mortality worldwide, highlighting the need for improved prognostic tools. The integration of artificial intelligence (AI) and machine learning (ML) with multi-omics data offers new opportunities for biomarker discovery and risk stratification. : We integrated bulk transcriptomic data from GSE116918 (training, = 248) and three cross-cohort consistency evaluation cohorts (TCGA-PRAD, GSE70769, GSE46602), focusing on 1087 epithelial-mesenchymal transition (EMT)-associated genes. Using consensus clustering, weighted gene co-expression network analysis (WGCNA), and 91 machine learning algorithm combinations (including Random Forest, Lasso, and CoxBoost), we constructed a prognostic signature. SHAP analysis was used for model interpretability. Single-cell RNA sequencing (scRNA-seq, GSE268307, 10,672 cells) and spatial transcriptomics (10× Genomics Visium FFPE) provided hypothesis-generating evidence; spatial analysis was based on one tissue section. : A three-gene signature (INHBA, FAP, ITGBL1) effectively stratified patients into high- and low-risk groups, with the high-risk group showing significantly worse metastasis-free survival (HR = 1.61, 95% CI: 1.39-1.87; 4-year AUC = 0.93 in the training cohort; external AUCs ranged from 0.62 to 0.77). CytoTRACE inferred high differentiation potential of COMP+ fibroblasts, and Monocle3 inferred a transcriptional transition from COMP+ toward NELL2+ fibroblasts. BayesPrism deconvolution suggested that high inferred COMP+ fibroblast abundance was associated with poor prognosis and advanced T stage. NicheNet analysis prioritized BMP7 as a key upstream ligand, with downstream targets enriched in TGF-β signaling and stem cell pluripotency pathways. : This study presents a machine learning-based multi-omics framework for prostate cancer risk stratification. The three-gene signature provides a new exploratory prognostic model while inferring a COMP+ to NELL2+ transcriptional transition. These findings may inform future hypothesis-driven studies of treatment sensitivity, pending experimental validation, and demonstrate the value of AI-driven multi-omics integration for precision oncology. - Source: PubMed
Publication date: 2026/08/27
Zhang XueqianZhang WeiWang ZhengShi XinyangZhang ChenghaoGao YanDeng YihengShen TianyuAn ZiyanFu Weijun - Craniosynostosis, resulting from the premature fusion of one or more cranial sutures, often requires invasive surgery for treatment. Currently, the spatiotemporal regulation of calvarial ossification during suture development is largely focused on cellular and molecular patterns. Using physicochemical imaging and nanomechanical mapping, we identified a unidirectional osteo-periosteal interface characterized by calcium phosphate deposition gradient during early postnatal calvarial development. Lineage tracing and dynamic bone formation assays revealed that Mmp13-positive osteoprogenitors, derived from Erg-expressing suture mesenchyme, establish this osteo-periosteal interface and drive asymmetric calvarial osteogenesis in mice. In the Twist1 craniosynostosis model, mineralization patterns initially resembled those of wild-type mice; however, misregulation of the PAK1/MMP13/BMP7 signaling cascade at the dura side triggered bilateral mineralization within the coronal suture, ultimately leading to bone fusion. Importantly, early localized inhibition of MMP13 overactivation, achieved by applying CL-82198 (a small-molecule MMP13 inhibitor) in a thin 20% gelatin methacryloyl (GelMA) hydrogel-based 'Suture Patch', preserved coronal suture patency in Twist1 mutants via a minimally invasive surgical procedure. Our work identifies a unilateral osteo-periosteal interface that is critical for coronal suture patency, reveals the regulatory role of non-cellular components in calvaria development, and proposes a minimally invasive strategy to preserve asymmetric osteogenesis in cranial sutures for the treatment of craniosynostosis. STATEMENT OF SIGNIFICANCE: Craniosynostosis is a premature skull suture fusion that currently requires invasive surgery. We discover a unidirectional mineralization gradient driven by Mmp13 osteoprogenitors. In a craniosynostosis model, this asymmetric process becomes bilateral and pathological. Leveraging this insight, we engineer a 200-μm hydrogel "Suture Patch" that delivers a small-molecule Mmp13 inhibitor locally. A single application in newborn mice preserves suture patency in 80% of treated animals, corrects skull deformity, and restores progenitor pools-without surgery. Our work integrates advanced materials characterization, single-cell biology, and translational bioengineering to provide both a fundamental revision of suture development and a practical, minimally invasive biomaterial therapy for the debilitating pediatric disorder. - Source: PubMed
Publication date: 2026/09/24
Meng HongxuWang XinyiWu BoxuanJiang YingqiDong YuningZhang TianyiChen BingqianLi HanzhangChen TaozehanLi WenyueWang XiaozhaoLan XinZhao TianruiHu HuanHuang WenwenGavara NúriaOuyang HongweiYuan Yuan - Surgically induced acute kidney injury (AKI) may involve both ischemia-reperfusion and direct mechanical damage to the renal parenchyma, potentially affecting subsequent tissue repair and remodeling. Although bone morphogenetic protein-7 (BMP-7) has shown renoprotective effects in experimental models of acute kidney injury, most studies have evaluated systemic administration in relatively homogeneous injury models. The effects of local BMP-7 delivery in the setting of combined ischemic and surgical parenchymal injury remain insufficiently defined. In this study, a rat model combining transient renal ischemia-reperfusion with a standardized parenchymal incision was used to investigate renal repair following surgical injury. Recombinant human BMP-7 (rhBMP-7) was applied locally to the injured renal parenchyma using Surgicel as a carrier. Serum renal function measures, tubular injury, proliferative activity, fibrosis-associated remodeling, and selected repair- and BMP-associated signaling markers were evaluated at 7 and 28 days after injury. Local Surgicel/rhBMP-7 application was associated with lower serum blood urea nitrogen and creatinine levels at day 7 compared with the suturing-only surgical-injury group. Histopathological tubular injury was observed in both groups, without significant between-group differences. Increased numbers of proliferating cell nuclear antigen (PCNA)-positive tubular epithelial cells were observed in the Surgicel/rhBMP-7 group at both analyzed time points. Compared with the suturing-only group, the Surgicel/rhBMP-7 group showed higher Wnt-4 immunoreactivity at both time points and higher phosphorylated Smad1/5/8 immunoreactivity at day 28, compatible with differences in BMP-associated signaling. Histological analysis demonstrated a reduced collagen-positive area in the Surgicel/rhBMP-7 group, accompanied by a time-dependent pattern of -SMA expression. In conclusion, local Surgicel/rhBMP-7 delivery was associated with differences in functional, tubular proliferative, repair-associated signaling, and histological remodeling responses following combined renal ischemia-reperfusion and surgical parenchymal injury. These findings support further evaluation of this localized delivery approach in more comprehensively controlled models of surgical kidney injury. - Source: PubMed
Publication date: 2026/09/09
Jakšić AnteBobinac DragicaMicek VedranĆelić Črnac TanjaBobinac MirnaOštrić MarinŠpanjol JosipPilipović Kristina - Open fractures require immediate irrigation and debridement to minimize the risk of contamination and support subsequent wound management. However, antiseptic irrigants may also exert local cytotoxic or osteogenesis-impairing effects. The objective of this study was to conduct a comparative analysis of the effects of hypochlorous acid (HOCl), povidone-iodine (PI), and boric acid-buffered Carrel-Dakin solution (CD) on fracture healing in a rat model of open femur fracture, compared to physiological saline (PS) solution. - Source: PubMed
Publication date: 2026/08/21
Tepedelenlioğlu Hüseyin EmreErol Gökçe Nur ArıkŞibar KemalElmas ÇiğdemCeylan Aslı FArıkan Şefik MuratKarasu Çimen