Ask about this productRelated genes to: Nanog protein
- Gene:
- NANOG NIH gene
- Name:
- Nanog homeobox
- Previous symbol:
- -
- Synonyms:
- FLJ12581, FLJ40451
- Chromosome:
- 12p13.31
- Locus Type:
- gene with protein product
- Date approved:
- 2003-09-10
- Date modifiied:
- 2014-11-19
Related products to: Nanog protein
Related articles to: Nanog protein
- : This study engineered and evaluated a targeted, folate-functionalized chitosan nanoparticle (CS-FA NP) delivery system to enhance the therapeutic efficacy of standard quercetin and seed extract against breast cancer. : Phytochemical profiling confirmed a 14% crude yield for the methanolic extract, with gas chromatography-mass spectrometry (GC-MS) and high-performance liquid chromatography (HPLC) identifying quercetin as the principal bioactive agent. The synthesized CS-FA NPs exhibited a core size of 7-20 nm, an average hydrodynamic diameter of 150-160 nm, a stable zeta potential of -55 mV, and high encapsulation efficiencies (87.2% for quercetin and 80.5% for coriander). Kinetic assessments confirmed a biphasic, diffusion-controlled release matching Higuchi matrix kinetics. Anticancer activity was evaluated in vitro using MTT cytotoxicity, Annexin V-FITC/PI apoptosis analysis, RT-qPCR, and ex vivo rat aortic ring assays, followed by validation in a syngeneic 4T1 mammary tumor mouse model. : In vitro, folate-receptor-targeted quercetin nanoparticles (T4) demonstrated superior, selective cytotoxicity, particularly against triple-negative MDA-MB-231 cells, while sparing normal fibroblasts. Annexin V-FITC/PI apoptosis profiling and ex vivo aortic ring assays revealed profound, cell-line-dependent programmed cell death and up to 90% inhibition of microvessel sprout outgrowth. Mechanistically, RT-qPCR verified that nano-formulations induced complete transcriptional silencing of NANOG, MMP-1, VEGFA, TSPAN8, TWIST, EMMPRIN, and CDK1, alongside marked upregulation of P27 and P21. In vivo, these nano-formulations successfully improved tumor-associated pathological features, reduced aggressive tumor spindle-cell proliferation, and suppressed elevated serum CA15-3 and arginase biomarkers. : Folate-functionalized chitosan nano-formulations significantly enhanced the anticancer efficacy of quercetin and seed extract through improved targeted delivery, potent antiproliferative, anti-angiogenic, and pro-apoptotic activities, together with favorable modulation of multiple molecular pathways associated with breast cancer progression. These findings support their potential as promising targeted nanotherapeutic strategies for breast cancer treatment. - Source: PubMed
Publication date: 2026/08/12
Nabil NarimanSabit HusseinAlmulhim JawaherArneth BorrosAbdel-Ghany Shaimaa - Because human infertility can arise from genetic, molecular, cellular, anatomical, and endocrine abnormalities, in vitro gametogenesis has become an important area of reproductive research. New developing technology involving the generation of human primordial germ cell-like cells (hPGCLCs) from induced pluripotent stem cells (hIPSCs) might assist with understanding early germ cell development (specification, migration, gametogenesis, and epigenetic reconstitution), as well as provide a solution for infertility and hereditary disorders. Given that human primordial germ cells (PGCs) are still not well characterized at a molecular level, we present a practical workflow to robustly and efficiently induce hPGCLCs from human pluripotent stem cells (hPSCs) XX and XY cell lines. This protocol describes the hPGCLC specification of hPSCs through sequential induction with Activin A for 2 days and BMP4 for 6 days in 2D and 3D culture systems. Induction of hPSCs into hPGCLCs demonstrated expression of early primordial germ cell markers, including PRDM1, NANOS3, DAZL, STELLA, SOX17, SSEA1, and cKIT, on the 8th day of hPGCLC generation. We described the protocol for generating early hPGCLCs, which provides an opportunity for further investigations into maturation into late germ cells and a chance to overcome a meiotic block to obtain haploid gametes in vitro. - Source: PubMed
Publication date: 2026/08/20
Abdyev Vepa KSirotkina Polina IErofeeva Evgeniia DErokhina Mariia AGrudinin Nikita YVorotelyak Ekaterina AVasiliev Andrey V - Glioblastoma (GBM) is the most aggressive primary brain tumor and remains associated with poor clinical outcomes despite advances in surgical and adjuvant therapies. The tumor microenvironment, particularly extracellular matrix (ECM) interactions, plays a crucial role in regulating glioblastoma progression, cellular plasticity, and therapeutic resistance. Therefore, physiologically relevant three-dimensional (3D) models are needed to better recapitulate GBM biology. In this study, we investigated the effects of ECM-functionalized polyacrylonitrile/coumarin-500 (PAN/C500) nanofiber scaffolds on the phenotype of LN-18 and U-87 MG glioblastoma cells cultured under 3D conditions. Cytoskeletal organization was assessed by phalloidin staining and live-cell vimentin imaging, while epithelial-mesenchymal transition (EMT)-associated proteins and stemness-related markers were analyzed by Western blotting. ECM-functionalized 3D PAN/C500 scaffolds promoted significant cytoskeletal remodeling, altered EMT-associated protein expression, and increased the expression of stemness-associated proteins, particularly SOX2, NANOG, and Nestin, compared with conventional 2D cultures. These responses were accompanied by cell line-dependent phenotypic adaptations, indicating that the engineered microenvironment influences glioblastoma cell behavior. This platform may serve as a valuable model for investigating glioblastoma biology and microenvironment-associated molecular adaptations in vitro. - Source: PubMed
Publication date: 2026/07/23
Nalkiran IhsanSevim Nalkiran HaticeAltuntas Derya BalMamuk Atilla ErenKocak CagdasCan EbihaAslan Sema - Humans are frequently exposed to diverse environmental pollutants that may pose potential risks during early embryonic development. Advances in stem cell toxicology have introduced new opportunities for environmental toxicity assessment, enabling the development of physiologically relevant models based on human induced pluripotent stem cells (iPSCs). However, current systems remain limited by low throughput and the inability to achieve real-time monitoring. In this study, we established a - iPSC reporter line by precisely inserting a T2A- cassette into the endogenous locus using CRISPR/Cas9, enabling dynamic and quantitative visualization of pluripotency status. Using this reporter system, we systematically evaluated the effects of 38 representative environmental pollutants spanning six major chemical categories, including bisphenols, per- and polyfluoroalkyl substances (PFAS), halogenated flame retardants, organophosphate esters, neonicotinoids, and phenolic compounds. Most pollutants induced varying degrees of reduction in - expression, suggesting perturbation of core transcriptional networks involved in pluripotency maintenance rather than nonspecific cytotoxic effects. Notably, bisphenol B (BPB), bisphenol Z (BPZ), perfluorobutanesulfonic acid (PFBS), tributyl phosphate (TnBP), tris-(2-chloroethyl) phosphate (TCEP), and 3,5-di-butyl-4-hydroxybenzoic acid (BHT-COOH) exhibited pronounced low-dose activity, causing significant suppression of signals even at 10 nmol/L. Furthermore, during neuroectoderm differentiation, several bisphenols and PFAS delayed the normal downregulation of , indicating disruption of the tightly regulated timing of pluripotency exit and lineage transition during early developmental progression. Overall, the - iPSC reporter model provides a sensitive, stable, and practical platform for high-throughput screening of environmental toxicants and offers mechanistically informed insight into how pollutant exposure may interfere with early human developmental regulatory programs. - Source: PubMed
Publication date: 2026/04/01
Zhao YanyiLi HanyueYang RenjunYin NuoyaFaiola Francesco - Pyrvinium pamoate, originally developed for protozoal infections, has emerged as a small-molecule inhibitor that interferes with Wnt/β-catenin signaling by suppressing β-catenin stability and downstream target gene activation. Wnt/β-catenin signaling pathway is a key intracellular regulatory mechanism of cellular proliferation and plays a particularly critical role in the maintenance of cancer stem cells. Accordingly, targeting cancer stem cells has become a major focus in the development of effective anticancer strategies, since these cells are primarily responsible for metastasis and disease relapse. Hispidulin is a natural flavonoid that has been shown to be effective on cell migration. In present study, PC3 castration-resistant human prostate cancer cells and RWPE-1 HPV-18-immortalized prostate epithelial cells were used. The antiproliferative, apoptotic and antimigrative effects of the combined use of pyrvinium pamoate and hispidulin on prostate cancer cells were analyzed by MTT test. The effects of hispidulin and pyrvinium pamoate on metastasis ability of PC3 cells were analyzed by wound healing assay. Proliferation- and cancer stem cell-related genes were analyzed by qPCR, and apoptotic bodies were visualized by Hoechst staining. Potential antimigratory effect of pyrvinium pamoate and hispidulin was showed not only in wound healing assay but also real time PCR. Besides, quantification analyses of mRNA synthesized from , , and genes exhibited that cancer stem cells were affected by hispidulin and pyrvinium pamoate. It was showed that the results were obtained will contribute to the development of stem cell-targeted methods in cancer treatment strategies. - Source: PubMed
Publication date: 2026/08/24
Serttas RizaErdogan Suat