Ask about this productRelated genes to: Nanog protein
- Gene:
- NANOG NIH gene
- Name:
- Nanog homeobox
- Previous symbol:
- -
- Synonyms:
- FLJ12581, FLJ40451
- Chromosome:
- 12p13.31
- Locus Type:
- gene with protein product
- Date approved:
- 2003-09-10
- Date modifiied:
- 2014-11-19
Related products to: Nanog protein
Related articles to: Nanog protein
- This study reports the generation and comprehensive characterization of the LATS1/2 knockdown (KD) induced pluripotent stem cell (iPSC) line, MUSIi012-A-9. Created via CRISPR/Cas9-mediated modification of the LATS2 gene in a parental LATS1-KD line, this resource serves as a crucial human model to investigate the roles of LATS1/2 kinases, key regulators of the Hippo signaling pathway. Comprehensive validation confirmed normal iPSC morphology, pluripotency marker expression (OCT3/4, NANOG, SOX2), genetic stability (46,XX karyotype), and robust multilineage differentiation potential. This well-characterized iPSC line is a vital tool for advancing research into Hippo pathway regulation, lineage specification, and cell fate determination in development and disease. - Source: PubMed
Publication date: 2026/06/29
Lorthongpanich ChanchaoSrisook PimonwanJiamvoraphong NittayaKlaihmon PhatchanatKumsui SirilukLaowtammathron ChutiIssaragrisil Surapol - Human multilineage-differentiating stress-enduring (MUSE) cells represent a promising cell population for tissue engineering and translational medicine owing to their intrinsic pluripotency and stress resistance. MUSE cells express pluripotency markers such as SSEA-3, Nanog, Sox2, and Oct3/4, while maintaining their immunomodulatory capabilities. This systematic review (PROSPERO CRD42024532621) analyzed studies on human MUSE cell isolation, characterization, and translational potential through searches of the PubMed, Scopus, and Web of Science databases in April 2024. The TIDieR and SYRCLE checklists were used to assess the risk of bias. Our findings identified 34 studies that followed the PRISMA guidelines for assessing different tissue sources, isolation techniques, and characterization methods. MUSE cells are primarily obtained from bone marrow mesenchymal stem cells, and fluorescence-activated cell sorting is the predominant isolation method. Characterization was mainly performed using SSEA-3 immunodetection (positivity ranged from <1% to 6.30%) and was linked to the expression of pluripotency and mesenchymal markers. These findings highlight the relevance of MUSE cells in regenerative medicine as a distinct pluripotent subpopulation within adult mesenchymal tissues. In addition, SSEA-3-based isolation approaches complemented by functional assays has emerged as a key methodological approach for the reliable identification and characterization of MUSE cells. However, isolation efficiency varies depending on the cell source and method. Standardized protocols are, therefore, needed to improve reproducibility and facilitate the translational development of MUSE cell-based regenerative therapies. - Source: PubMed
Garzón IngridMartín-Piedra Miguel ÁngelMartínez-Girón LuisAlaminos MiguelYoo James JAtala Anthony - Mesenchymal progenitor cells (MPCs) play a significant role in articular cartilage homeostasis and regeneration. Yet, the functional dynamics and molecular characteristics of MPCs may differ significantly across various pathological conditions. Hence, this study comprehensively investigates the biological and molecular characteristics of MPCs isolated from articular cartilage of patients with osteoarthritis (OA) and rheumatoid arthritis (RA), aiming to uncover disease-specific differences that could offer insights into targeted regenerative therapies. Using flow cytometry, gene expression analysis, and in vitro differentiation assays, we assessed the phenotype, growth potential, senescence, cytogenetic instability, and chondrogenic potential to delineate molecular pathways uniquely active in each disease context. Phenotypically, both OA and RA-MPCs retained markers of mesenchymal stem cells (MSCs), but OA-derived MPCs exhibited higher fold expression of progenitor markers (, , , and ), suggesting a more activated state. Functionally, OA-MPCs demonstrated increased growth kinetics (higher proliferation rate and decreased population doubling time) with a significant shift towards adipogenic lineages (increased fold expression of , , and ). However, there were no differences in the osteogenic and chondrogenic potential. Gene expression analysis revealed upregulation of genes involved in extracellular matrix production and cartilage development (, , , , , , , , and ) in 3D cultures compared with 2D or monolayer cultures. Collectively, these findings demonstrate that, while multipotent MPCs are present in both OA and RA articular cartilage, they can exhibit fundamentally altered biological behaviors and molecular signatures reflective of the local disease microenvironment. Understanding these differences is critical for optimizing cell-based therapeutic strategies tailored to each condition and may facilitate the development of novel interventions targeting endogenous progenitor cells for cartilage repair. - Source: PubMed
Publication date: 2026/06/10
Manjappa Akshay BairapuraNitilapura NarendraShetty SiddharthRao ShamaBabu SanthoshShetty JayaprakashaShetty ReshmaBasavarajappa Mohana Kumar - Understanding how the first cell lineages in human development are specified and maintained has fundamental importance and clinical implications for regenerative medicine, infertility and pregnancy loss. While mouse models have provided valuable insights into transcription factors regulating early development, translating these findings to human embryos has been limited by ethical, technical and biological constraints. Functional studies of transcription factors in human embryos have been hindered by nuclease-based genome-editing approaches that induce genotoxicity. To overcome this, we applied adenine base editing (ABE8e) to precisely target an exon splice donor site, resulting in a splicing defect and functional knockout of NANOG, representing the first application of base editing to study a developmental regulator in human embryos. This approach did not trigger genotoxicity and showed limited off-target editing. Loss of NANOG disrupts pluripotent epiblast specification and instead cells differentiate toward a primitive endoderm (yolk sac) or trophectoderm (placental) transcriptional programme. Retention of primitive endoderm differentiation in NANOG-edited human embryos reveals a functional compensation distinct from mouse, underscoring the importance of directly investigating human development. Our findings demonstrate an essential role for NANOG in human pluripotency and epiblast specification, and highlight the utility of base editing for functional interrogation of human development. - Source: PubMed
Publication date: 2026/06/25
Bower Oliver JR Orsi Ana EMcMahon RileyStaneva DesislavaBlagrove JosephineSingh KashishSimon Claire SMcCarthy AfshanGarcia PatriciaShaikly ValerieTaranissi MohamedWilding MartinSerhal PaulOdia Rabi AVasilic MinaChoudhary MeenakshiPapathanasiou AthanasiosElder KaySnell PhilChristie LeilaArbab MandanaLiu David RHerbert MaryHarasimov KatarinaNiakan Kathy K - iPSC technology is well established in mammals but remains underdeveloped in non-mammalian species. A major barrier to generating avian iPSCs has been the lack of species-specific reprogramming factors and culture conditions capable of supporting self-renewal in avian pluripotent stem cells. Here, we report the generation of chicken iPSCs (ciPSCs) using a cocktail of seven chicken transcription factors (T7: Oct4, Sox2, Sox3, Klf4, c-Myc, Nanog, and Lin28B) combined with an optimized avian culture system. Transcriptomic and functional analyses identified Sox3, rather than Sox2, as the predominant SoxB1 factor in avian reprogramming. The resulting ciPSCs exhibited stable self-renewal for over 40 passages, expressed core pluripotency markers, differentiated into all three germ layers, and were transcriptionally similar to chicken ESCs. In chimera assays, ciPSCs contributed to somatic, extra-embryonic, and germline lineages, giving rise to gonadal PGC-like cells that did not acquire full germline competence. We further demonstrate that the T7 system generates iPSCs from quail, duck, peacock, zebra finch, and pigeon, and that duck iPSCs can form interspecies chimeras with donor cells detected in the host gonads. These findings establish a generalizable platform for avian iPSC generation with applications in developmental biology and germline preservation of endangered species. - Source: PubMed
Publication date: 2026/06/16
Tong XinyiChen XiAnicete ArleneChan YanpuiZhou XuanWang XiziMcKim Daniel BYing Qi-Long