Ask about this productRelated genes to: MMP9 antibody
- Gene:
- MMP9 NIH gene
- Name:
- matrix metallopeptidase 9
- Previous symbol:
- CLG4B
- Synonyms:
- -
- Chromosome:
- 20q13.12
- Locus Type:
- gene with protein product
- Date approved:
- 1990-03-14
- Date modifiied:
- 2015-02-23
Related products to: MMP9 antibody
Related articles to: MMP9 antibody
- Amyloid-β (Aβ), particularly the aggregation-prone Aβ1-42, plays a central role in Alzheimer's disease (AD). While its neuronal toxicity is well known, effects on glial and vascular cells remain unclear. To investigate how Aβ1-42 oligomers affect oligodendrocyte precursor cells (OPCs), pericytes (PCs), and endothelial cells (ECs), and how these effects contribute to blood-brain barrier (BBB) dysfunction. In vitro assays were used to assess cell viability and BBB integrity following Aβ exposure. Transcriptomic profiling was performed on Aβ1-42-treated OPCs. Transendothelial electrical resistance (TEER) was used to measure barrier function. Aβ1-42, but not Aβ1-40, induced cytotoxicity in OPCs and PCs. ECs showed impaired barrier function without cell death. Aβ1-42-treated OPCs upregulated pro-inflammatory genes (Mmp9, Il1b) and downregulated genes related to cell cycle and growth signaling. Conditioned media from Aβ-exposed OPCs and PCs reduced TEER in ECs, indicating paracrine-mediated BBB disruption. These findings demonstrate that Aβ1-42 oligomers impair BBB integrity under in vitro conditions through both direct and non-cell autonomous mechanisms. Further in vivo studies are warranted to validate the relevance of these mechanisms in AD pathogenesis. - Source: PubMed
Publication date: 2026/06/19
Toyokawa MasaruYasuda KenKikuya AkihiroAsada-Utsugi MegumiHida MisakiNakamura YamatoYanagida NarufumiToda ShintaroKaji SeijiKinoshita YusukeOno YuichiTakahashi RyosukeMatsumoto RikiKinosita AyaeMaki Takakuni - Osteoporosis is characterized by increased bone fragility and elevated fracture risk, severely compromising patients' quality of life. This study aimed to investigate the therapeutic potential of isoorientin (Iso) against osteoporosis and elucidate its underlying mechanisms. Using a combination of in vitro approaches, we demonstrated that Iso effectively inhibited RANKL-induced osteoclast differentiation in RAW 264.7 cells and downregulated the expression of key osteoclastic markers-including matrix metallopeptidase 9 (MMP9), cathepsin K (CTSK), c-fos, and nuclear factor of activated T-cells 1 (NFATc1)-at both protein and mRNA levels. Iso also exhibited potent anti-inflammatory and antioxidant activities, reducing the levels of pro-inflammatory cytokines (IL-6, TNF-α, IL-1β) and malondialdehyde (MDA) while enhancing superoxide dismutase (SOD) activity and glutathione (GSH) content. Mechanistically, Iso acts by suppressing the ROS/PPARγ/NF-κB signaling axis. In vitro, the anti-osteoclastogenic effect of Iso was attenuated by the PPARγ agonist, and either the PPARγ antagonist or the NF-κB inhibitor BAY 11-7082 alone effectively inhibited osteoclast differentiation. In an ovariectomy (OVX)-induced osteoporotic mouse model, Iso treatment significantly prevented bone loss, improved bone microarchitecture, and suppressed osteoclast activity. Notably, administration of the PPARγ antagonist GW9662 or the NF-κB inhibitor BAY 11-7082 alone also exerted clear bone-protective effects in vivo, further validating these two pathways as viable therapeutic targets for osteoporosis. In conclusion, isoorientin exerts anti-osteoporotic effects by inhibiting the ROS/PPARγ/NF-κB signaling pathway, with efficacy comparable to clinical drugs, offering a promising novel candidate for the management of osteoporosis. - Source: PubMed
Publication date: 2026/06/08
Zhan YunzhongYang FanWu YichenHuang WeichengMao YiLiu HongdongZhu Minyu - To clarify the therapeutic targets and signaling pathways of total flavone (AATF) in gout treatment, we integrated network pharmacology and experiments. Network pharmacology was applied to screen AATF's anti-gout targets, construct protein-protein interaction (PPI) and drug-component-disease-target-pathway networks, and conduct gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses. A dual gout rat model was established using potassium oxonate, adenine, and monosodium urate (MSU) crystals, followed by assessments including toe swelling measurement, hematoxylin-eosin (HE) staining of kidney and joint tissues, digital radiography (DR) imaging, detection of serum uric acid and xanthine oxidase (XOD) activity, enzyme-linked immunosorbent assay (ELISA) of inflammatory factors, and Western blot validation of key targets. Network pharmacology revealed that AATF modulates inflammatory responses and the tumor necrosis factor (TNF) pathway via core targets including albumin (ALB), TNF, interleukin-6 (IL-6), and tumor protein 53 (TP53). experiments showed that AATF significantly ameliorated renal and joint pathological damage, reduced serum uric acid/XOD activity, downregulated serum interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), IL-6, cyclooxygenase-2 (COX-2) levels, and inhibited joint nuclear factor kappa B (NF-κB), extracellular signal-regulated kinase 1 (ERK1), matrix metalloproteinase 9 (MMP9) expression. Collectively, AATF exerts anti-gout effects through multi-target and multi-pathway mechanisms linked to the TNF signaling pathway, providing critical preliminary evidence for its preclinical development. - Source: PubMed
Publication date: 2026/06/18
Wang YilinWang ShuangLi ZhaoxiaChen XiZhang ChengyiHan XiaoxueWan LimeiFan SuiqiangLiu YitingGuo ZiyanHuang XiaoqiangXu XiaozeZhou XinXing Bingfeng - Honey is a natural sweet substance produced by bees from nectar and plant secretions, which is abundant in nutrients and exhibits excellent biological activities. However, owing to deficiencies in the quality control index system, prominent issues such as honey adulteration and improper processing are prevalent in the current market, resulting in a severe decline in honey quality. Therefore, this study focuses on acacia honey, one of the four major honey varieties in China, by screening its characteristic components and analyzing the correlation between these components and immunomodulatory activity, aiming to provide targeted theoretical basis and indicator support for resolving the aforementioned practical issues. The results demonstrated that natural acacia honey (NAH) had significant advantages over commercial acacia honey (CAH) in key quality indices (total flavonoids, total phenols, proline content, amylase activity, etc.) and quality consistency, with significantly higher immunomodulatory activity. Combined with machine learning and chemometric analyses, hesperetin and pinocembrin were confirmed as the characteristic components of NAH from the 50 components identified via ultra-performance liquid chromatography-quadrupole-electrostatic field orbitrap mass spectrometry (UPLC-QE-MS) analysis. In vitro experiments demonstrated that both components at 50 μM effectively promoted mouse splenic lymphocyte proliferation, reduced NO and TNF-α secretion in LPS-induced RAW264.7 macrophages, and showed no obvious cytotoxicity, indicating their potential as characteristic markers for evaluating NAH quality and functional activity. Further network pharmacology and molecular docking studies indicated that hesperetin and pinocembrin could mediate immunomodulatory effects by regulating 10 core targets including MMP9, MMP2, BCL2, and SRC, as well as the PI3K-AKT pathway, with the markers showing the strongest binding activity toward MMP2. This study established a characteristic marker identification system for NAH, and provided scientific indicator support for formulating acacia honey quality standards and improvement of processing technologies. - Source: PubMed
Publication date: 2026/06/17
Wang BingkangHua MinLiu YuechengLi MengZhang NingWu YiGao QiSun HongfuDai YanpengZhou MengZhou Qian - Johne's disease (JD), caused by subsp (MAP), is a chronic enteric disease affecting cattle worldwide, with considerable economic implications for dairy producers. MAP is a robust, intracellular pathogen highly resilient to environmental challenges. To elucidate the molecular mechanisms underlying MAP infection in cattle (), we performed a comprehensive transcriptomic analysis. - Source: PubMed
Publication date: 2026/06/03
Sahu AbhisekAbdullah MohdGupta SaurabhSingh Shoor VirDhillon AnkushYadav PrabhatiAzam Sarwar